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Insect Cell Culture Using a BioFlo 110 Benchtop Bioreactor

Gas mode)
PID values: P = 2.00 and I = 0.67

Gas Control (O2 loop)
The control was set to 4-Gas mode, to automatically maintain DO and pH setpoints, using 3 gases (Air, O2, N2) to control the DO setpoint and CO2 to control pH setpoint.

Controller Setup:
O2 Control Selections: 4 Gas mode

Continuous Feed
All pumps were calibrated using the standard supplied tubing to keep track of the liquid quantities entering and exiting the vessel. Samples were taken several times a day to measure glucose and cell density.

Controller Setup:
Pump 1 . . . . . . . . . . .plugged into "Pump A" Power Controller outlet
Pump 2 . . . . . . . . . . .plugged into "Pump B" Power Controller outlet
Pump 1 (Nutrient) . ..Vessel inlet - Triport adapter in the vessel headplate
Pump 2 (Harvest) . . .Vessel outlet - 12mm port using the dip tube
Pump A Selection . . .Manual (automatically adds liquid to the vessel with the Bio- Command control program)
Pump B Selection . . .Wet On (removes liquid from the vessel using the level probe as a trigger to turn the pump on)

Control Program
For this study, we used BioCommand Plus software to control nutrient addition. For the nutrient control program, we calculated the approximate timeframe profile to automatically add fresh media using pump 1. After 2 days of batch process, medium perfusion was started at the rate of 0.5 - 2L working volumes per day.

Results and Discussion
Insect cells generally have


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