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Insect Cell Culture Using a BioFlo 110 Benchtop Bioreactor


Introduction
This Application Report is one in a series documenting simple protocols for culturing cells in a BioFlo 110 benchtop fermentor/bioreactor system. The modular BioFlo 110 is offered with a variety of vessels, impellers and control modules to enable growth of a wide variety of cultures, including mammalian, insect, plant, yeast and bacterial cells. Complete kits are also available. This report concentrates on achieving high-density insect cell cultures, using our Advanced Cell Culture Kit. For this application, we used a standard 3.0L BioFlo 110 Advanced Cell Culture Kit with a magnetic drive and a water-jacketed vessel (NBS Catalog No. M1273- 1212). A Suspension-Cell Spin Filter, with 16-18 micron screen (NBS Catalog # M1273-3202) was used for a high-flow-rate perfusion to grow the insect cells in a continuous mode. Additionally, BioCommand Plus supervisory software (NBS Catalog No. M1291-0000) was used to control the feeding schedule. Insect cells are used to produce a variety of proteins by utilizing the Baculovirus Expression Vector System (BEVS). Insect cell lines such as Sf9, Sf21, Hi-5, etc., are proven to express high levels of end products.


The Bioreactor
Vessel
The BioFlo 110 Advanced Cell Culture Kit was equipped with a magnetic drive and water-jacketed 3.0 L vessel with a nominal working volume of 2.25 L. All BioFlo 110 Advanced Cell Culture Kits are configured with a pitched-blade agitation impeller, as well as dissolved oxygen and pH probes (Mettler Toledo), three liquid addition bottles, cables, tubing, clamps and other useful items. Optional direct drive and magnetic-drive agitation systems, as well as water-jacketed and heat-blanketed vessels are also available in the cell culture kits.
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