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Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun

size of 1.6 m were the best for both plants. The number of micro carriers and amount of viral cDNA tested did not affect infection efficiency.

We suggest the starting conditions indicated in Table 2 to optimize viral RNA or cDNA inoculation of tobacco and potato plants. They have been used successfully for many more recent studies in our laboratory (Hamalainen et al. in press, Rajamaki and Valkonen 1999).


References
Armaleo, D., et al., Biolistic nuclear transformation of Saccharomyces cerevisiae and other fungi, Curr. Genet., 17, 97103 (1990)

Bartels, R., Potato virus A, CMI/AAB Desc. Pl. Viruses , 54 (1971)

Brunt, A. A., The general properties of potyviruses, Arch. Virol., 5, 3-16 (1992)

Gal-On, A., et al., Particle bombardment drastically increases the infectivity of cloned DNA of zucchini yellow mosaic potyvirus, J. Gen. Virol., 76, 32233227 (1995)

Gilbertson, R. L., et al., Cloning of the complete DNA genomes of four bean-infecting geminiviruses and determining their infectivity by electric discharge particle acceleration, Phytopathology, 81, 980985 (1991)

Hamalainen, J. H., et al., Recessive and dominant genes interfere with the vascular transport of potato virus A in diploid potatoes, Mol. Plant Microbe Interact. (in press).

Klein R. M., et al., High-velocity microprojectiles for delivering nucleic acids into living cells, BioTechnology, 24, 384-386 (1992)

Matthews, R. E. F., Plant Virology (3rd ed.), Academic Press, London (1995)

Puurand, , et al., Infectious in vitro transcripts from cloned cDNA of the potato A potyvirus, Virus Res., 40, 135140 (1996)

Rajamaki, M.
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