Navigation Links
Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun

.

In each optimization step, three cartridges of PVA-T7 transcript (RNA) or PVA-35S (cDNA) were bombarded onto tobacco and potato plants. Each test was carried out at least three times. After each optimization step, the optimal parameter was used in further experiments, as explained above. Significance of observed differences was tested using analysis of variance and calculation of the least significant differences (Steel and Torrie 1981).

Results
Pressure and Distance. In tobacco, 100% infection was achieved using a helium pressure of 150 or 200 psi at a distance of 0 cm, but extensive death of the cells in the middle of the bombarded area was observed (Figure 2). At the higher pressure of 250 or 300 psi, leaves tended to be ripped. No infection was detected in tobacco plants bombarded at the low pressures of 80 and 100 psi, and at these pressures the bombarded areas usually remained undamaged. Results were similar for PVA-T7 (RNA) and PVA-35S (cDNA).

In the potato clone A6, infection initiation sites could be visually identified by the development of necrotic lesions as a result of the hypersensitive response to PVA. A few necrotic lesions characteristic of PVA infection (Valkonen et al. 1995) were observed at 80 psi, and their numbers increased with increased pressure (Figure 3). At lower pressures (80 and 100 psi), the bombarded area of leaf was usually undamaged and just a few necrotic lesions were observed. Bombardment with higher pressures (150 and 200 psi) led to cell death in the middle part of the bombarded tissue, but numerous necrotic lesions were still observed in the surrounding surviving tissue (Figure 4).


'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System
2. Versatile Reporter Vectors for Monitoring Viral Transduction
3. Maximize Viral RNA Yield from Biological Fluid
4. Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus
5. Isolation and Characterization of Anti-Viral Nucleoside Compounds from the Chinese Herb Taraxacum Mongolicum
6. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
7. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
8. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
9. Oil-Free PCR Using the Hot Top Assembly
10. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:3/4/2015)... CALGARY , March 4, 2015 /CNW/ ... that it has completed a collaborative research ... quantitative analytics.  Using Fast Collective Evolution Technology ... dataset including all measurements obtained from each ... Company,s Phase 2 clinical trials ASSERT, SUSTAIN ...
(Date:3/4/2015)... March 04, 2015 Proove ... Personalized Medicine, is excited to announce the launch ... to Optimize the Management of Pain”. , The ... Medscape.com for the next year. The focus of ... to recognize inadequate pain treatments, integrate appropriate pain ...
(Date:3/4/2015)... 2015  BioClinica®, Inc., a leading specialty ... announced that clinical trial professionals representing 20 ... annual European User Conference in ... use of its eClinical technologies to overcome ... the Microsoft Office-Smart OnPoint CTMS; Express EDC; ...
(Date:3/4/2015)... Jose, California (PRWEB) March 04, 2015 ... Reaction (PCR) represents one of the fastest growing technologies ... cell biologists in subcellular visualization is driving growth in ... need for deeper understanding of gene expressions will continue ... market. Expanding applications in fields ranging from pharma, biotech, ...
Breaking Biology Technology:Resverlogix Collaborates with Emerald Logic to Identify Factors Driving Drug Response and Efficacy in Phase 2 Program 2Resverlogix Collaborates with Emerald Logic to Identify Factors Driving Drug Response and Efficacy in Phase 2 Program 3Resverlogix Collaborates with Emerald Logic to Identify Factors Driving Drug Response and Efficacy in Phase 2 Program 4Proove Biosciences Launches CME Accredited Program with Medscape 2Proove Biosciences Launches CME Accredited Program with Medscape 3BioClinica Teams With Pharma To Tackle Clinical Trial Challenges In Europe 2BioClinica Teams With Pharma To Tackle Clinical Trial Challenges In Europe 3Focus on Quantifying Nucleic Acid Molecules Spurs Growth in the qPCR & dPCR Instrumentation Market, According to a New Report by Global Industry Analysts, Inc. 2Focus on Quantifying Nucleic Acid Molecules Spurs Growth in the qPCR & dPCR Instrumentation Market, According to a New Report by Global Industry Analysts, Inc. 3Focus on Quantifying Nucleic Acid Molecules Spurs Growth in the qPCR & dPCR Instrumentation Market, According to a New Report by Global Industry Analysts, Inc. 4
... the rap against Midwest technology networking events: you pay to ... you spend the evening talking to all the service providers ... to these things very often. Why dont they go? Because ... meet them at networking events, pitch their services and bombard ...
... the tools your competitor has may be your companys ... Overture allow advertisers to purchase company and product names ... This allows companies to poach potential customers from their ... searches for a competitor by name. , ,The ...
... from an experimental CIMSS satellite. Courtesy of UW-Madison. ... big as the weather, the University of Wisconsin's ... bigger computer. , ,The institute has begun testing its ... supercomputer offered by Silicon Graphics Inc. , a ...
Cached Biology Technology:Wake up and smell the networking coffee 2Wake up and smell the networking coffee 3Wake up and smell the networking coffee 4Beware of trademark poachers in search-engine ads 2Beware of trademark poachers in search-engine ads 3Beware of trademark poachers in search-engine ads 4UW-Madison picks scalable SGI computer to simulate weather 2UW-Madison picks scalable SGI computer to simulate weather 3
(Date:1/22/2015)... , Jan. 15, 2015  BellBrook Labs, a leader ... announced the launch of a TR-FRET (time resolved ... Transcreener UDP Assay, a high throughput screening assay ... assay will allow for sensitive detection of hundreds ...
(Date:1/22/2015)... 20, 2015 NXT-ID, Inc. (NASDAQ: NXTD and NXTDW) ... on the recent success of the Wocket™ smart wallet at CES 2015 ... wallet was named as one of the "11 Hot Products at CES" ... Launched At CES So Far" by Newseveryday.com and "The top 10 gadgets ...
(Date:1/22/2015)... Jan. 21, 2015  Analyst Report Issued by Small Cap IR. ... showed that planet-wide, transactions at merchants on the leading payment ... number of credit, debit, and prepaid cards reached 6.54 billion.  ... of fraudulent card usage in 2012, and credit and debit ...
Breaking Biology News(10 mins):BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 3$4.80 Near Term Price Target with "Buy" given by CapRock Research for NXT-ID, Inc. (NASDAQ: NXTD) issued by Small Cap IR 2
... May Control Staph Infections in Humans Including MRSA ... bacteriophage active against Staphylococcus aureus, including methicillin-resistant strains, ... their findings in the August 2007 issue of ... aureus is a highly flexible and potentially dangerous ...
... papermaking industry,s black liquor waste into synthesis gas is a ... footprint of coal-to-liquid processes, and can produce a fuel versatile ... to a team of Penn state engineers. "Black liquor is ... of fuel science. "But it has more energy value as ...
... Current Biology, a publication of Cell Press, reports some of ... that men and women differ when it comes to their ... prefer pinkor at least a redder shade of bluethan men ... were surprised at how robust they were, given the simplicity ...
Cached Biology News:Tips from the Journals of the American Society for Microbiology 2Tips from the Journals of the American Society for Microbiology 3Coal and black liquor can produce energy from papermaking 2Girls prefer pink, or at least a redder shade of blue 2
... increasing age, the autofluorescent pigment lipofuscin ... cell types, including neurons. The presence ... use of fluorescence microscopy in the ... broad excitation and emission spectra, which ...
... S100 belongs to the family of calcium binding ... S100A is composed of an alpha and beta ... chains. S100 protein is also expressed in ... cells in skin and interdigitating reticulum cells in ...
LEAF™ Purified anti-human/mouse/rat ICOS...
Peptide-affinity Purified Polyclonal Antibody to MTR...
Biology Products: