Navigation Links
Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun

tent of mechanical damage on bombarded areas of the leaves was observed. Symptoms of PVA infection in A6 (necrotic lesions) were observed four days after bombardment. In tobacco, PVA infection was detected twelve days after bombardment of upper uninoculated leaves.

The assay was a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using monoclonal antibodies and alkaline phosphatase-conjugated monoclonal antibodies to PVA (MAb 58/0; Rajamaki et al. 1998) obtained from Adgen, UK.

Optimization of Bombardment Parameters. We tested various bombardment conditions in order to identify those that resulted in the largest number of necrotic lesions on A6 leaves and in infection of all inoculated tobacco plants. First, different helium pressures (80, 100, 150, 200, 250 and 300 psi) were tested using 2 g PVA-35S or PVA-T7 per mg gold (1.0 m), 0.05 mg PVP per ml 99.5% ethanol, and 0.5 mg gold per bombardment as recommended for starting conditions in the Helios gene gun instruction manual. Bombardments were done with 0 or 2 cm distance between the spacer and the target. The first experiments showed that bombardment at 250 and 300 psi damaged leaves extensively, so these pressures were not included in further experiments.

Next, PVP concentrations were varied (0, 0.05 and 0.1 mg per ml 99.5% ethanol). Third, using the previously determined optimal helium pressure and PVP concentration, different sizes of microcarriers (0.6, 1.0 and 1.6 m) were tested. In the last experiment, various amounts of microcarriers per shot (the microcarrier loading quantity, or MLQ) and various amounts of PVA cDNA coated on microcarriers (the DNA loading ratio, or DLR) were tested
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System
2. Versatile Reporter Vectors for Monitoring Viral Transduction
3. Maximize Viral RNA Yield from Biological Fluid
4. Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus
5. Isolation and Characterization of Anti-Viral Nucleoside Compounds from the Chinese Herb Taraxacum Mongolicum
6. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
7. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
8. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
9. Oil-Free PCR Using the Hot Top Assembly
10. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:12/24/2014)... 2014 PharmaBoardroom,s new report, ... and available for free download , digs deep into ... growth in the sector today. One area where ... been in developing a homegrown pharmaceutical manufacturing base, even if ... remains some way off. A cursory comparison with neighboring ...
(Date:12/24/2014)... NEW YORK , Dec. 23, 2014 ... pilot study of mazindol in children with attention deficit/hyperactivity ... in December 2014 . The paper, ... with attention deficit/hyperactivity disorder" ( Konofal et al, ... 1;8:2321-2332. eCollection 2014 ) shows that mazindol might ...
(Date:12/22/2014)... 2014 /PRNewswire/ - RepliCel Life Sciences Inc. (OTCQB: REPCF) ... on the development of autologous cell therapies, announced today ... of Calgary in conjunction with co-authors from Kyoto University ... the company,s ongoing clinical research using dermal sheath cup ... The paper entitled " Hair Follicle Dermal ...
(Date:12/22/2014)...  ( www.competitivehealth.com ) — Competitive Health is pleased ... and advocacy service, has signed an agreement to become ... health services marketplace. 63% of Americans have ... expected to pay. As part of an ongoing effort ... is pleased to offer medical bill review and negotiation ...
Breaking Biology Technology:PharmaBoardroom Releases New Algeria Pharmaceuticals Report 2PharmaBoardroom Releases New Algeria Pharmaceuticals Report 3Redefining ADHD: A New Approach & A Shift of Paradigm in ADHD Therapeutics 2Redefining ADHD: A New Approach & A Shift of Paradigm in ADHD Therapeutics 3Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 2Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 3Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 4Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 5WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2
... PRA, a leading Clinical Research Organization, continues our expansion ... of an office in Singapore. The office will serve ... strengthening the quality and timeliness of our deliverables to ... the heart of the city-state, will provide monitoring, project ...
... PARK, Calif., Feb. 23, 2012 Telome Health, Inc.™, ... telomere biology in human health, has announced that co-founder and ... Year by Silicon Valley Business Journal. ... award, especially coming from the heart of innovation, Silicon Valley, ...
... Calif., Feb. 21, 2012 Keck Graduate Institute (KGI) ... (CGS) and the Alfred P. Sloan Foundation to administer ... part of establishing and maintaining high quality standards for ... honored to be selected and we look forward to ...
Cached Biology Technology:PRA Opens Office in Singapore 2Telome Health Co-founder and Nobel Laureate Elizabeth Blackburn Awarded Innovator of the Year 2KGI Selected to Administer PSM Affiliation Process 2
(Date:12/24/2014)... NEW YORK , Dec. 23, 2014  Since its ... has helped thousands of people to eliminate the pain ... replacing the antiquated system with their own biometrics fused ... to remember usernames and passwords, Hoyos Labs , ... identity, announced today that it is offering the app ...
(Date:12/22/2014)... SHELTON, Conn. , Dec. 22, 2014  NXT-ID, ... or the "Company"), a biometric authentication company focused on ... famous pickpocket, entertainer and security consultant, Apollo Robbins ... Wocket™ biometric smart wallet.  Apollo ... 6th and 7 th , 2015, demonstrating some of ...
(Date:12/22/2014)... Dec. 22, 2014 Research and Markets ... the addition of the "The Global ... their offering. ... an overview of the global digital media ... control and monitor piracy by securely and ...
Breaking Biology News(10 mins):1U Offers Best Solution to the Username / Password Dilemma: For FREE! 21U Offers Best Solution to the Username / Password Dilemma: For FREE! 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4The Global Watermarking and Fingerprinting Markets 2
... Atlantis rockets into space later this week, it will ... study how their genetic responses and their ability to ... part of the STS-115 space shuttle mission scheduled for ... Salmonella typhimurium, Pseudomonas aeruginosa and Candida albicans -- that ...
... Virginia Health System have shown for the first time ... muscle cells can reverse myotonic dystrophy, the most common ... 40,000 people in the United States have myotonic muscular ... wasting of the muscles, irregular heartbeat, cataracts and insulin ...
... the Rubeho Mountains in Tanzania revealed over 160 ... eleven endemic species--according to an article published in ... findings elevate the importance of protecting this biologically-rich ... range from destructive activities underway such as clear-cutting ...
Cached Biology News:Microscopic passengers to hitch ride on space shuttle 2Microscopic passengers to hitch ride on space shuttle 3Toxic molecule may cause most common type of muscular dystrophy 2First surveys of Tanzanian mountains reveal 160+ animal species, including new & endemic 2
...
... The ideal modular cleanroom for biological ... that require cleanliness (to Class 10/ISO ... precision-joined to create ultra-smooth surfaces that ... disinfect. One-inch radius corners simplify cleaning. ...
This free-standing work station safely ventilates fumes upwards, away from the work surface. Optional Exhaust Purification System features bonded charcoal filters that remove organic chemical vapors ...
... Analysis Note: Protein determined by ... 3.2 M (NH 4 ) ... 7.0 Preparation Note: Affinity purified ... suspension • Additives: MgCl 2 ...
Biology Products: