Navigation Links
Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun

Tuija Kekarainen and Jari P. T. Valkonen, Department of Plant Biology, Swedish University of Agricultural Sciences (SLU), P.O. Box 7080, SE-75007 Uppsala, Sweden


Introduction
Particle bombardment is a physical method widely used for gene transfer into plants, mammals, fungi and bacteria (Armaleo et al. 1990, Klein et al. 1992, Shark et al. 1991, Williams et al. 1991, Zelenin et al. 1989). The hand-held Helios gene gun provides wider possibilities for gene transfer to plant tissue both in vivo and in vitro, because it is not limited by the large size of the target, and bombardment does not need to be carried out under vacuum.

The particle bombardment technique has been used to infect plants with DNA viruses, RNA viruses and cloned RNA viruses (Gal-On et al. 1995, Gilbertson et al. 1991, Klein et al. 1992 ) . The PDS-1000/He system is efficient for inoculation of detached leaves, but the Helios gene gun enables inoculation of an intact plant. In this study we have used the Helios gene gun to inoculate whole tobacco and potato plants with the cDNA of an RNA virus, potato virus A (PVA; Puurand et al. 1996). We wanted to optimize bombardment parameters to achieve 100% efficiency of PVA infection in susceptible tobacco plants. In the case of potato plants, we used potato clone A6 (Solanum demissum Lindl. x S . tuberosumcv. Aquila), which develops necrotic lesions at the site of PVA infection (Valkonen et al. 1995 ), and thus enables counting of the number of initial infection sites. The number of lesions can be used as a readily observed measure for optimal inoculation conditions. The bombardment parameters examined in this study were helium pressure, distance of target tissue from the s
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System
2. Versatile Reporter Vectors for Monitoring Viral Transduction
3. Maximize Viral RNA Yield from Biological Fluid
4. Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus
5. Isolation and Characterization of Anti-Viral Nucleoside Compounds from the Chinese Herb Taraxacum Mongolicum
6. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
7. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
8. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
9. Oil-Free PCR Using the Hot Top Assembly
10. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:1/15/2014)... 2014 The Microcompetition with Foreign DNA ... disease. One of these latent viruses is the Epstein ... rheumatoid arthritis (RA). Rheumatoid arthritis (RA) is a chronic ... by the theory, a study found that RA patients ... (1). , What is Microcompetition? , Dr. Hanan ...
(Date:1/15/2014)... Sunnyvale, CA (PRWEB) January 15, 2014 ... of high throughput research solutions, today announced that ... companies, has received delivery of Freeslate’s CM ... form screening. , Lupin, headquartered in Mumbai, ... quality, affordable generic and branded formulations and Active ...
(Date:1/14/2014)... (PRWEB) January 14, 2014 Date: Friday, ... p.m. , Location: Warrington Country Club, 1360 Almshouse Road, ... Foundation, the only national nonprofit organization solely dedicated to ... quality of life for those affected worldwide, will host ... Warrington Country Club in Warrington, Pa. , Each ...
(Date:1/14/2014)... , Jan. 14, 2014  3D Communications, a leading provider ... major scientific, regulatory, business, and media events in ... , today announced its former associate Virginia Cox , ... office. Virginia Cox re-joins ... Commissioner for the Office of External Affairs at the U.S. ...
Breaking Biology Technology:Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2Former FDA Associate Commissioner Returns To 3D Communications 2
... ... initiative to create the next generation of fertilizers and production technologies, with initial financial ... ... -- IFDC is launching the Virtual Fertilizer Research Center (VFRC), a global ...
... , ... ... ... , ...
... ... ... ... ...
Cached Biology Technology:IFDC Launches Virtual Fertilizer Research Center 2IFDC Launches Virtual Fertilizer Research Center 3IFDC Launches Virtual Fertilizer Research Center 4IFDC Launches Virtual Fertilizer Research Center 5Icahn Nominates 4 to Genzyme Slate 2Icahn Nominates 4 to Genzyme Slate 3Icahn Nominates 4 to Genzyme Slate 4Icahn Nominates 4 to Genzyme Slate 5Icahn Nominates 4 to Genzyme Slate 6Icahn Nominates 4 to Genzyme Slate 7Icahn Nominates 4 to Genzyme Slate 8Icahn Nominates 4 to Genzyme Slate 9Icahn Nominates 4 to Genzyme Slate 10Longhorn Vaccines & Diagnostics LLC Receives FDA Emergency Use Authorization (EUA) for the First 2009 H1N1 Influenza Assay to Include a Molecular Transport Medium (MTM) 2Longhorn Vaccines & Diagnostics LLC Receives FDA Emergency Use Authorization (EUA) for the First 2009 H1N1 Influenza Assay to Include a Molecular Transport Medium (MTM) 3Longhorn Vaccines & Diagnostics LLC Receives FDA Emergency Use Authorization (EUA) for the First 2009 H1N1 Influenza Assay to Include a Molecular Transport Medium (MTM) 4Longhorn Vaccines & Diagnostics LLC Receives FDA Emergency Use Authorization (EUA) for the First 2009 H1N1 Influenza Assay to Include a Molecular Transport Medium (MTM) 5Longhorn Vaccines & Diagnostics LLC Receives FDA Emergency Use Authorization (EUA) for the First 2009 H1N1 Influenza Assay to Include a Molecular Transport Medium (MTM) 6
(Date:4/15/2014)... high sea levels will rise into the next century, ... University scientist Rene Price. , Price is also affiliated ... Long-Term Ecological Research (LTER) site, one of 25 such ... deserts, mountains to salt marshes around the world. , ... the most certain consequences of climate change. , But ...
(Date:4/15/2014)... University of Missouri researchers have previously shown that ... to exercise exists. In a new study, Frank Booth, ... has found a potential link between the genetic pre-disposition ... at which mental maturation occurs. , For his study, ... extreme activity or extreme laziness. Booth then put the ...
(Date:4/15/2014)... the deadliest infectious diseases recognized the contributions of Frederick ... University of Texas Medical Branch, with a Lifetime Achievement ... The filoviruses include Ebola and Marburg viruses that cause ... The current outbreak of Ebola virus raging in West ... , "This award represents the culmination of a ...
Breaking Biology News(10 mins):Long-term predictions for Miami sea level rise could be available relatively soon 2Genetic pre-disposition toward exercise and mental development may be linked 2International research group recognizes UTMB experts 2International research group recognizes UTMB experts 3
... the diaphragm muscles has received approval from the Food and ... Sclerosis (ALS), also known as Lou Gehrig,s disease. The ... patients breathe longer without the aid of a ventilator. ... Medical Center who helped develop and test DPS and who ...
... in the October 2011 print issue of The FASEB ... what kicks off the process that causes varicose veins. In ... binds to DNA to control gene function (called "transcription factor ... of proteins that significantly affect the development of varicose veins. ...
... work of a team of scientists at Cold Spring Harbor ... way to overcome one of the major technical obstacles preventing ... addressed successfully by novel drugs. The target is ... long known to be an important player in the signaling ...
Cached Biology News:Diaphragm pacing system receives FDA approval for use with ALS patients 2Scientists discover the proteins that control development of varicose veins 2Powerful antibody-based strategy suggests a new therapeutic approach to diabetes and obesity 2Powerful antibody-based strategy suggests a new therapeutic approach to diabetes and obesity 3Powerful antibody-based strategy suggests a new therapeutic approach to diabetes and obesity 4
... Gene expression profiling allows a high-throuput screening ... naturally occuring or artifitially induced changes in ... that system can be detected, studied, classified ... broad range of genes at a glance. ...
... SYBR Green II RNA gel stain ... stain that has bright fluorescence when bound ... making it ideal for use with either ... or standard UV transilluminators. A 500 µL ...
Rabbit polyclonal to Esa1 ( Abpromise for all tested applications)....
... RNA gel stain is a sensitive nucleic ... bound to RNA and low background in gels, ... or polyacrylamide gels using laser scanners or standard ... 500 µL unit size (S-7564) and a special ...
Biology Products: