Navigation Links
Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun

Tuija Kekarainen and Jari P. T. Valkonen, Department of Plant Biology, Swedish University of Agricultural Sciences (SLU), P.O. Box 7080, SE-75007 Uppsala, Sweden


Introduction
Particle bombardment is a physical method widely used for gene transfer into plants, mammals, fungi and bacteria (Armaleo et al. 1990, Klein et al. 1992, Shark et al. 1991, Williams et al. 1991, Zelenin et al. 1989). The hand-held Helios gene gun provides wider possibilities for gene transfer to plant tissue both in vivo and in vitro, because it is not limited by the large size of the target, and bombardment does not need to be carried out under vacuum.

The particle bombardment technique has been used to infect plants with DNA viruses, RNA viruses and cloned RNA viruses (Gal-On et al. 1995, Gilbertson et al. 1991, Klein et al. 1992 ) . The PDS-1000/He system is efficient for inoculation of detached leaves, but the Helios gene gun enables inoculation of an intact plant. In this study we have used the Helios gene gun to inoculate whole tobacco and potato plants with the cDNA of an RNA virus, potato virus A (PVA; Puurand et al. 1996). We wanted to optimize bombardment parameters to achieve 100% efficiency of PVA infection in susceptible tobacco plants. In the case of potato plants, we used potato clone A6 (Solanum demissum Lindl. x S . tuberosumcv. Aquila), which develops necrotic lesions at the site of PVA infection (Valkonen et al. 1995 ), and thus enables counting of the number of initial infection sites. The number of lesions can be used as a readily observed measure for optimal inoculation conditions. The bombardment parameters examined in this study were helium pressure, distance of target tissue from the s
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System
2. Versatile Reporter Vectors for Monitoring Viral Transduction
3. Maximize Viral RNA Yield from Biological Fluid
4. Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus
5. Isolation and Characterization of Anti-Viral Nucleoside Compounds from the Chinese Herb Taraxacum Mongolicum
6. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
7. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
8. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
9. Oil-Free PCR Using the Hot Top Assembly
10. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:7/30/2015)... According to a new market ... PB/PK, PKPD, Trial Design, Toxicity Prediction, In-house, Contract Service), ... by End Users - Global Forecast to 2020", published ... USD 2,107.99 million by 2020 from USD 1,034.93 million ... Browse more than 75 ...
(Date:7/30/2015)... Denmark , July 30, 2015 ... biotechnology company that applies its innovative TransCon technology ... positive top-line results from a six-month Phase 2 ... once-weekly TransCon Growth Hormone in 53 treatment-naïve, pre-pubertal ... "We are extremely pleased with the top-line ...
(Date:7/29/2015)... Sanofi, a global and ... quarter of 2015. CEO Olivier Brandicourt ... in different businesses. Watch video interview ... Topics covered in the interview include:  ... Performance drivers - Diabetes ...
(Date:7/29/2015)... MONTREAL , July 29, 2015 /PRNewswire/ - BioAmber Inc. ... Laurin , the Company,s Chief Financial Officer has resigned.  ... former Chief Financial Officer who retired in December 2014, ... search for a permanent successor.     Andy ... Mr. Laurin and ensure a smooth transition.  Andy was ...
Breaking Biology Technology:Biosimulation Market Worth 2,107.99 Million USD by 2020 2Biosimulation Market Worth 2,107.99 Million USD by 2020 3Biosimulation Market Worth 2,107.99 Million USD by 2020 4Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 2Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 3Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 4Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 5Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 6Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 7BioAmber Announces Change in Chief Financial Officer 2BioAmber Announces Change in Chief Financial Officer 3
... Marshfield Clinic has reached an agreement to ... TeraMedica , a Milwaukee-based medical informatics company. , ... to integrate all of Marshfield's clinical data, including ... the clinic's electronic medical record. , ,This will ...
... a national reputation for its emerging biotech industry, ... launch pad for intellectual property (IP) lawsuits. , ... In the highly competitive science and technology community, ... tools used to protect discoveries and innovation. The ...
... Baird Venture Partners , the U.S. venture ... on its presence in China by partnering with the Indian ... portfolio companies as they establish a foothold in the Indian ... partner of Baird Venture Partners and director of Baird Private ...
Cached Biology Technology:Rocket docket speeds patent infringement suits 2Rocket docket speeds patent infringement suits 3Rocket docket speeds patent infringement suits 4Rocket docket speeds patent infringement suits 5Visions: Carbone bullish on Wisconsin venture capital 2Visions: Carbone bullish on Wisconsin venture capital 3Visions: Carbone bullish on Wisconsin venture capital 4Visions: Carbone bullish on Wisconsin venture capital 5
(Date:7/23/2015)... Research and Markets ( http://www.researchandmarkets.com/research/rzzp3v/global_outlook_of ) ... Outlook of the Biometrics Industry 2015 " ... global biometrics market is likely to witness significant ... to the uptake of multiple technologies available. The ... the growth of biometrics in existing applications as ...
(Date:7/13/2015)... 13, 2015 Synaptics Inc. (NASDAQ: ... solutions, today announced sampling of ClearPad ® ... integration (TDDI) product targeting smartphones and tablets. This ... Synaptics , best-in-class touch controller IP and ... developed in the company,s Japan Design Center. TDDI ...
(Date:7/9/2015)... SAN JOSE, Calif. , July 9, ... ), the leading developer of human interface ... authentication technology, the industry,s first fully hardware ... innovative Match-in-Sensor secure authentication technology is literally ... pattern storage and biometric matching within the ...
Breaking Biology News(10 mins):Global Outlook of the Biometrics Industry 2015 2Global Outlook of the Biometrics Industry 2015 3Synaptics Announces Sampling of Second Generation TDDI Solutions 2Synaptics Announces Sampling of Second Generation TDDI Solutions 3Synaptics Announces World's First Match-in-Sensor Fingerprint Authentication Technology 2Synaptics Announces World's First Match-in-Sensor Fingerprint Authentication Technology 3
... Polytechnic University of Catalonia (UPC) have developed a method ... impact of storms. The method, which has been applied ... region,s coasts have a high rate of vulnerability to ... "Until now there was no tool for evaluating coastal ...
... Seattle Rice which provides nearly half the daily ... climate change and some catastrophic events by colonizing its seeds ... just-published U.S. Geological Survey-led research shows. In an ... to climatic scourges such as tsunamis and tidal surges that ...
... of nature have long known that crops like corn and ... control this nightly stem elongation, common to most plants, have ... this week,s early online publication of the journal Nature, ... their discovery of a protein complex they call the "evening ...
Cached Biology News:One-third of central Catalan coast is very vulnerable to storm impact 2Climate adaptation of rice 2Climate adaptation of rice 3Biologists discover an 'evening' protein complex that regulates plant growth 2Biologists discover an 'evening' protein complex that regulates plant growth 3Biologists discover an 'evening' protein complex that regulates plant growth 4Biologists discover an 'evening' protein complex that regulates plant growth 5
... Serum • Mouse serum is collected off the clot ... weeks of age. Available Anticoagulants: N-02: ... K2EDTA N-08: Potassium Oxalate N-10: ... ACD N-07: CPD N-09: ...
Recombinant Mouse Wnt-3a...
Contains 100 positive controls for effective screening of HDAC inhibitors by fluorometric method....
... There are at least 20 structurally ... of the TGF-beta superfamily. BMPs were originally ... bone formation. They are also involved in ... organs. BMPs regulate the growth, differentiation, chemotaxis ...
Biology Products: