Navigation Links
Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun

Tuija Kekarainen and Jari P. T. Valkonen, Department of Plant Biology, Swedish University of Agricultural Sciences (SLU), P.O. Box 7080, SE-75007 Uppsala, Sweden


Introduction
Particle bombardment is a physical method widely used for gene transfer into plants, mammals, fungi and bacteria (Armaleo et al. 1990, Klein et al. 1992, Shark et al. 1991, Williams et al. 1991, Zelenin et al. 1989). The hand-held Helios gene gun provides wider possibilities for gene transfer to plant tissue both in vivo and in vitro, because it is not limited by the large size of the target, and bombardment does not need to be carried out under vacuum.

The particle bombardment technique has been used to infect plants with DNA viruses, RNA viruses and cloned RNA viruses (Gal-On et al. 1995, Gilbertson et al. 1991, Klein et al. 1992 ) . The PDS-1000/He system is efficient for inoculation of detached leaves, but the Helios gene gun enables inoculation of an intact plant. In this study we have used the Helios gene gun to inoculate whole tobacco and potato plants with the cDNA of an RNA virus, potato virus A (PVA; Puurand et al. 1996). We wanted to optimize bombardment parameters to achieve 100% efficiency of PVA infection in susceptible tobacco plants. In the case of potato plants, we used potato clone A6 (Solanum demissum Lindl. x S . tuberosumcv. Aquila), which develops necrotic lesions at the site of PVA infection (Valkonen et al. 1995 ), and thus enables counting of the number of initial infection sites. The number of lesions can be used as a readily observed measure for optimal inoculation conditions. The bombardment parameters examined in this study were helium pressure, distance of target tissue from the s
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System
2. Versatile Reporter Vectors for Monitoring Viral Transduction
3. Maximize Viral RNA Yield from Biological Fluid
4. Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus
5. Isolation and Characterization of Anti-Viral Nucleoside Compounds from the Chinese Herb Taraxacum Mongolicum
6. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
7. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
8. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
9. Oil-Free PCR Using the Hot Top Assembly
10. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:9/2/2014)... NEW PROVIDENCE, N.J. , Sept. 2, 2014 ... corporate sponsor for MassBio,s 20th Annual Golf Classic, a ... Foundation. The event will be held on September 5 ... Plymouth, Massachusetts . The MassBioEd ... Massachusetts through educational programs, workforce development, and ...
(Date:9/2/2014)... Press attending the Project LIBERTY Grand Opening ... the people and events in Emmetsburg on Sept. 3. ... the day. Additionally, we will do our best to ... as well as representatives from the local community. Any ... Please remember to RSVP to Matt Merritt (matt.merritt@poetdsm.com) to ...
(Date:9/2/2014)... Opertech Bio, Inc., a company ... effective approach to sensory evaluation, today announced the ... U.S. Patent and Trademark Office, encompassing high-throughput sensory ... “Opertech’s taste evaluation technology called Microtiter Operant Gustometer ... system for rapid characterization of taste sensory properties,” ...
(Date:9/2/2014)... 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the Company") ... consist of three large odour control systems for waste water ... "These orders are consistent with the market resurgence in ... President and CEO. "After a difficult period of restraint in ... to see sales growth. Our lower cost structure, technology improvements ...
Breaking Biology Technology:Linde helps fund STEM education in Massachusetts 2Linde helps fund STEM education in Massachusetts 3Press agenda for Project LIBERTY Grand Opening 2Press agenda for Project LIBERTY Grand Opening 3Press agenda for Project LIBERTY Grand Opening 4Press agenda for Project LIBERTY Grand Opening 5Opertech Bio Receives U.S. Patent for High-Throughput Taste Evaluation Technology 2
... Health, a three-hospital health system providing care ... information management system provided by Surgical Information ... seamlessly into the hospitals, existing Siemens Soarian® ... safety and drive business results. ...
... September 29, 2010 ... ... var ... longUrl, so we need to grab the first one. for (var r in data.results) { first_result = data.results[r]; ...
... his significant and global contributions to clean water and ... his commitment to invention and the pursuit of discovery, ... degree from Stevens Institute of Technology at the university,s ... Meng, a professor in the Charles V. Schaefer, Jr. ...
Cached Biology Technology:Susquehanna Health Selects Perioperative and Anesthesia Automation Solution from Surgical Information Systems 2Susquehanna Health Selects Perioperative and Anesthesia Automation Solution from Surgical Information Systems 3Susquehanna Health Selects Perioperative and Anesthesia Automation Solution from Surgical Information Systems 4InSightec Launches ExAblate(R) One and ExAblate(R) OR Systems at CIRSE 2010 2InSightec Launches ExAblate(R) One and ExAblate(R) OR Systems at CIRSE 2010 3InSightec Launches ExAblate(R) One and ExAblate(R) OR Systems at CIRSE 2010 4InSightec Launches ExAblate(R) One and ExAblate(R) OR Systems at CIRSE 2010 5InSightec Launches ExAblate(R) One and ExAblate(R) OR Systems at CIRSE 2010 6InSightec Launches ExAblate(R) One and ExAblate(R) OR Systems at CIRSE 2010 7InSightec Launches ExAblate(R) One and ExAblate(R) OR Systems at CIRSE 2010 8InSightec Launches ExAblate(R) One and ExAblate(R) OR Systems at CIRSE 2010 9InSightec Launches ExAblate(R) One and ExAblate(R) OR Systems at CIRSE 2010 10Xiaoguang Meng receives honorary master of engineering from Stevens 2Xiaoguang Meng receives honorary master of engineering from Stevens 3
(Date:9/2/2014)... truth; everyone has a tipping point. We all want to ... benefit is great enough. Now, scientists have confirmed the area ... , The result was published online this week in ... honest, even if lying is beneficial," said Lusha Zhu, the ... Virginia Tech Carilion Research Institute , where she works with ...
(Date:9/2/2014)... Sam Kean stops by Reactions this week to debunk ... that, despite what "Shark Week" may lead you to ... http://youtu.be/KhFygIoW_MA . , Kean,s book, "The Disappearing Spoon: ... History of the World from the Periodic Table of ... 10-episode video series produced for the newly launched American ...
(Date:9/2/2014)... for eating anything, including each other, camel crickets are ... Carolina State University finds that non-native camel cricket species ... , "The good news is that camel crickets don,t ... says Dr. Mary Jane Epps, a postdoctoral researcher at ... the research. , The research stems from a chance ...
Breaking Biology News(10 mins):Scientists find possible neurobiological basis for tradeoff between honesty, self-interest 2Scientists find possible neurobiological basis for tradeoff between honesty, self-interest 3Scientists find possible neurobiological basis for tradeoff between honesty, self-interest 4Researchers find Asian camel crickets now common in US homes 2
... Johns Hopkins researchers report that two proteins best known ... the liver into a sugar-producing factory when food is ... damaging problem in people with diabetes, the proteins' interaction ... the disease, the researchers say. , Under normal circumstances, ...
... the immune system uses to label foreign invaders as ... system can brand foreign proteins by chemically modifying their ... cells known as lymphocytes would recognize the trespassers and ... T cells need to see these types of modifications ...
... a close relative of the Formosan "super termite" that's ... become established in South Florida, according to University of ... pest in tropical areas such as Brazil and the ... threat to Florida until now," said Nan-Yao Su, a ...
Cached Biology News:Future diabetes drugs may target new protein interaction 2Future diabetes drugs may target new protein interaction 3Newly Discovered 'Branding' Process Helps Immune System Cells Pick Their Fights 2Newly Discovered 'Branding' Process Helps Immune System Cells Pick Their Fights 3Cousin of Asian super termite invades Florida 2
Human LT beta R/TNFRSF3 Phycoerythrin MAb (Clone 71319)...
Human Serpin A3 MAb (Clone 213907)...
Recombinant Ovine (Sheep) Growth Hormone, Ultra Pure...
Human SorCS1 Affinity Purified Polyclonal Ab Protein Family: Neurotrophins...
Biology Products: