( 2 t1) Where GU...)Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System

2 t1)

Where GUR is the Glucose Uptake Rate in mg/hour
C_Glu is the concentration of residual glucose
t1 & t2 are the sampling hours
V_solu is the volume of media in liters

We recommend maintaining the GUR at > 1,000 mg/L.

NucleoCounter Automated Cell Counter - Or, use an automated cell counting device, like the NucleoCounter, available in the US exclusively through New Brunswick Scientific. The NucleoCounter is an automated cell counting device specifically designed to provide fast and accurate counts of mammalian and insect cells, without regard to cell size or morphology. (A NucleoCounter YC-100 model is also available for automated counts of yeast cells.) The NucleoCounter systems rely on use of a disposable plastic sampling cartridge the NucleoCassette, which contains a nucleus staining dye, propidium iodide. To use, aseptically remove a few samples of the FibraCel disks and remove the cells from the disks. How cell removal is accomplished is dependent on whether you are you are culturing suspension or anchorage-dependent cells:

Suspension cells are usually released from the FibraCel disks very easily. Place the disks in a small test tube containing 5 mL of phosphate-buffered saline (PBS) and shake, sonicate or vortex. The cells should fall off the disks. Remove the PBS and centrifuge the liquid; re-suspend the cells in 0.5 mL PBS.

Anchorage-dependent cells may require sonication or typsinization to remove the cells from the FibraCel disks. For trypsinization, place the disks in a test tube. Using a standard typsinization buffer, incubate for 30 seconds, remove the liquid, add 1 mL PBS and incubate at 37C for 8 minu
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