Navigation Links
Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System

should be added as required.


pH Measurements & CO2 Concentrations:
Due to the high cell densities typically achieved in FibraCel culture, the pH levels in the medium may fluctuate in the later stages of cell growth.

Adjust the culture medium pH to a range of between 7.2 to 7.4 for mammalian cell culture, 6.2 to 6.4 for Sf-9 insect cells, and 6.4 to 6.5 for Hi-5 cells.

When growing mammalian cell cultures, a pH level above 7.6 may slow attachment of your cells to the disks. If the culture medium contains 2.2 g/L NaHCO3, set the CO2 to 5%. If pH is below normal range, adjust the CO2 concentration to 0, and/or increase the NaHCO3 concentration to 3.7 g/L. Culture medium containing 25 mM HEPES can also stabilize the pH during culture.

In insect cultures, when the pH is below 6.0, add 1M of Bis-Tris to increase the pH to 6.5. Increasing the Up/Down Speed and Bottom Hold Time will help to expel CO2, reduce lactate production, and thus further stabilize pH in culture medium.

Measure the pH regularly, and adjust the CO2 concentration accordingly in the incubator.


Media Consumption:

The medium consumption is process dependent. Expect to use 1 to 3 L of media for virus production in semibatch culture. In processes where you are continuously collecting conditioned culture medium for secreted protein production, you may use 5 to 10 L of media to extend the culture to about 15 days.


Media Replenishment
Replenish or modify the culture media as necessary:

Glucose Concentration - Change the medium when the glucose concentration falls below 1.0 ~ 1.5 g/L (initial glucose concentration should be > 3.5
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
2. Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor
3. An Easy-to-Use Research Fermentor/Bioreactor
4. Chemical Stability of Disposables
5. Big Blue Rodent Cell Cultures for Mutagenesis Studies
6. Simple Isolation of RNA from Tissue and Cultured Cells
7. The QIAGEN Guide to Animal Cell Culture
8. The QIAGEN Guide to Animal Cell Culture
9. The QIAGEN Guide to Animal Cell Culture
10. The QIAGEN Guide to Animal Cell Culture
11. The QIAGEN Guide to Animal Cell Culture
Post Your Comments:
(Date:8/19/2014)... 20, 2014 One of the ... is an inadequate vascular supply. Nutrient and gas ... tissues is critical for successful regeneration and integration ... mitigate deficiencies in vascularization and promote angiogenesis in ... optimizing scaffold design and architecture, and enhancing scaffolds ...
(Date:8/19/2014)... (PRWEB) August 19, 2014 Shimadzu ... chromatography systems, Prominence-i and Nexera-i, adding to the ... Combining excellent functionality, an intuitive operating environment, and ... a more efficient workflow for conventional to ultra-high-speed ... innovative, intuitive and intelligent design so users can ...
(Date:8/19/2014)... Research and Markets  has announced the addition of the ... report to their offering. This report analyzes the ... by the following Product Segments: Media, Sera, and Reagents. ... Canada , Japan , Europe ... America . Annual estimates and forecasts are provided for the ...
(Date:8/19/2014)... 19, 2014 A Geisinger researcher on a ... a Centers for Disease Control and Prevention (CDC) award ... health concern. Joseph Boscarino , Ph.D. ... a team of researchers nominated for the Centers for ... Award for Data Methods and Study Design. ...
Breaking Biology Technology:Vascularization in Tissue Engineering Highlighted at 12th New Jersey Symposium on Biomaterials Science 2Shimadzu’s New i-Series Integrated Liquid Chromatography Systems Provide Laboratories Wider Range of Analytical Capabilities 2Shimadzu’s New i-Series Integrated Liquid Chromatography Systems Provide Laboratories Wider Range of Analytical Capabilities 3Global Cell and Tissue Culture Supplies - Strategic Business Report 2014 2Geisinger investigator nominated for federal award 2Geisinger investigator nominated for federal award 3
... Advanced Instruments, Inc., of,Norwood, Massachusetts, announced today ... D & F Control Systems, Inc., of Dublin, ... D & F Control Systems manufactures and ... laboratories around the world. Their,patented preservatives ensure component ...
... Operations Appointed -, CLINTON, N.J., Oct. 2 ... Michael Kennedy as Senior Vice President,Engineering and Operations. ... technology strategy and development., "Michael,s extensive experience ... as we continue to advance our pharmaceutical,pipeline through ...
... the largest non-state-owned producers of ... plasma-based product in China, ... (OTC Bulletin Board: CBPO) ("China Biologic" or the,"Company"), one of ... ("PRC"), today announced that on September 26,2008, the Company has ...
Cached Biology Technology:Advanced Instruments Acquires D & F Control Systems Inc. 2Ikaria Strengthens Executive Leadership Team 2China Biologic Products Enters Into Agreement to Acquire 90% Controlling Interest in Chongqing Dalin Biologic Technologies Co., Ltd. 2China Biologic Products Enters Into Agreement to Acquire 90% Controlling Interest in Chongqing Dalin Biologic Technologies Co., Ltd. 3China Biologic Products Enters Into Agreement to Acquire 90% Controlling Interest in Chongqing Dalin Biologic Technologies Co., Ltd. 4China Biologic Products Enters Into Agreement to Acquire 90% Controlling Interest in Chongqing Dalin Biologic Technologies Co., Ltd. 5
(Date:8/19/2014)... have identified a biomarker strongly associated with basal-like ... resistant to many types of chemotherapy. The biomarker, ... for new therapeutics designed to treat this often ... taken from The Cancer Genome Atlas, molecular biologists ... powerful computational and bioinformatics techniques to detect patterns ...
(Date:8/19/2014)... ancestors of today,s crocodiles colonised the seas during warm ... a new Anglo-French study which establishes a link between ... over a period of more than 140 million years. ... from the Universit de Lyon, France and formerly from ... in Nature Communications ., Today, crocodiles are ,cold-blooded, ...
(Date:8/19/2014)... fizzy soda knows that bubbles can throw tiny particles ... industrial applications, Princeton researchers have demonstrated that the bursting ... well. , "It is well known that bursting bubbles ... to discover that when we covered the water with ... the water," said Howard Stone, the Donald R. Dixon ...
Breaking Biology News(10 mins):Biomarker in an aggressive breast cancer is identified 2Biomarker in an aggressive breast cancer is identified 3Evolution of marine crocodilians constrained by ocean temperatures 2Bubbling down: Discovery suggests surprising uses for common bubbles 2Bubbling down: Discovery suggests surprising uses for common bubbles 3
... wringing every drop of energy from as many photons ... chemistry, materials science and electronic engineering researchers on a ... but existing techniques are now running up against limits ... Now, researchers from the University of Pennsylvania and Drexel ...
... BURLINGTON, Mass. , Nov. 12, 2013  Decision Resources, ... for pharmaceutical and healthcare issues, finds that while most ... play a critical role in pharmaceutical development of chronic ... outside of oncology is building. The Strategic Insights report ...
... more meat, milk and eggs in their diets. Increased ... livestock production and massive environmental consequences at local, regional ... Treatment and Management, draws from Danish expertise and spotlights ... work and ensure the use of animal manure,s stinking ...
Cached Biology News:Penn and Drexel team demonstrates new paradigm for solar cell construction 2Penn and Drexel team demonstrates new paradigm for solar cell construction 3Penn and Drexel team demonstrates new paradigm for solar cell construction 4Penn and Drexel team demonstrates new paradigm for solar cell construction 5Use of Biomarkers in Clinical Trials for Drugs Outside of Oncology is Increasing 2New textbook tackles the sustainable recycling of manure 2
... SB3, Fully adjustable mixing angle, Variable speed, ... hold a number of different sized tubes, ... aerating cultures, keeping biological samples in suspension ... can be used in incubators up to ...
...
Fingerprinting II database sharing tools is an optional module for the Fingerprinting II Basic software package that enables peer-to-peer data exchange over the internet and provides external databas...
Fingerprinting II cluster analysis is an optional module that adds dendrogram and phylogenetic tree construction, cluster and group significance, and congruence of techniques functions to the Fingerp...
Biology Products: