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Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System

he platform is lowered, the bellows expand, allowing the media to recede, leaving the disks covered by a thin liquid film. The film helps with oxygen transfer and removal of excess CO2. The entire device fits into a standard CO2 incubator for control of temperature and gasses, and the controller is magnetized for convenient storage on the outside wall of the incubator.

FibraCel disks have a proven record of high-yield production of cells, virus and secreted proteins from a wide variety of cell lines, including hybridoma, CHO, VERO, BHK, and insect cells2. These disks are also currently being used in the production of the worlds first licensed gene therapy drug, a treatment for head and neck squamous cell carcinoma. New Brunswick Scientific offers a wide range of bioreactor systems incorporating the FibraCel packed-bed technology in 0.5 to 150 Liter working volume capacities, but FibraStage is the first of their single-use systems for cell culture.


The Effect of Media on Cell Attachment
We have found that approximately 85% of suspension cells and 100% of anchorage-dependent cells weve tested will readily attach to FibraCel disks. If in doubt, the most economical way to determine if your cells will attach to the disks is to experiment in T-flasks. In the US and parts of Europe, NBS also offers FREE 30-day in-lab trials of the FibraStage system. Ask your NBS representative for details.


Inoculation
Once youve verified that your cell line will grow using FibraCel disks, inoculate one to four FibraStage bottles, as desired. Begin with an inoculum of at least 1 2 x 108 cells per bottle for mammalian cell cultures, and 1.5 2 x 108 cells per bottle for insect cell cultures. (See Table 2.) Higher cell densities will sho
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