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Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System


Introduction

New Brunswick Scientific now offers a new labor-saving device for producing mg quantities of proteins, virus or cell mass from anchorage-dependent or suspension cultures. More productive than traditional roller bottles, spinners or T-flasks1, the new FibraStage system is inexpensive, simple to operate, and disposable. Typical yields of 4.9 x 109 cells per 500 mL disposable FibraStage bottle have been achieved with a variety of cells types. The system holds up to four bottles, enabling simultaneous screening of four different cell lines or media compositions.


About the FibraStage Single-Use Cell Culture System
At the heart of the FibraStage system are pre-sterilized, single-use bottles, with a cell growth chamber filled with 10 grams of FibraCel disks. FibraCel is a three-dimensional growth substrate, composed of polypropylene and polyester non-woven fiber, which provides an extremely high-surface area for cell growth. Anchorage-dependent and suspension cultures become attached to or embedded in the FibraCel disk bed, where they remain throughout the growth process. Immobilization within the disk bed not only protects the cells from damage by shear forces, but also simplifies harvesting, allowing the supernatant to be easily recovered from the cell-free medium. The bottles come pre-sterilized and ready to use, requiring no cleaning, autoclaving or validating, for further reduction of labor.


The FibraStage system includes a variable-speed platform into which the FibraStage bottles are placed. When the platform is raised, bellows at the base of each bottle are compressed, forcing media over the cells, facilitating nutrient uptake and removal of cell waste. When t
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