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Innovative Tissue Array Technology for High-Throughput Screening of Gene,,,Expression

and goat anti-rabbit FITC as the secondary antibody. The result of this immunological screening assay demonstrates the actin-binding protein within the muscle tissue (Figure 3).

Fig.2

Fig.3

Since many applications of tissue array technology rely on nucleic acid assays, we used the Complete View male mouse validation array slides for an in situ hybridization experiment (Figure 4). One of two slides was treated with RNase prior to hybridization of both slides with a fluorescently labeled poly(dT) probe. Salivary gland tissue on the slide without RNase digestion clearly shows the presence of mRNA . We used standard conditions for slide preparation, prehybridization, and hybridization reactions (Methods).

Fig.4

Conclusions

The Complete View tissue array slide kits permit wide- spread use of tissue array technology. With the single-slide format and convenient configuration of tissues, these innovative array slides simplify screening procedures so specialized expertise in histology is not required. We demonstrated their use to localize expression to specific tissues and cells, and our data confirm that these optimized array slides maintain the morphological integrity of tissues. By immunostaining of the tropomyosin protein and fluorescent labeling of RNA, we verified that the tissues affixed to the Complete View slides retain antigenicity of proteins and reactivity of nucleic acids, respectively. We are expanding this product line to include human fetal validation and survey arrays.

Methods

In Situ Hybridization: Paraffin was removed from the array tissue by treating the slides as described in the product instructions. The two consecutive slides
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Related biology technology :

1. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
2. Simple Isolation of RNA from Tissue and Cultured Cells
3. Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer
4. Hands-free Tissue Disruption No Homogenization Needed
5. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
6. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
7. Stabilizing RNA in Fresh and Frozen Tissues for RNA Isolation
8. Genomic DNA Preparation from RNAlater Preserved Tissues
9. DNA Isolation From 300 to 450 mg Dried or 600 to 1,200 mg Fresh Leaf Tissue
10. DNA Isolation From 50 to 20 mg of Paraffin-Embedded Tissue
11. Genomic DNA Isolation From 510 mg Marine Invertebrate Tissue
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