Navigation Links
Innovative Tissue Array Technology for High-Throughput Screening of Gene,,,Expression

and goat anti-rabbit FITC as the secondary antibody. The result of this immunological screening assay demonstrates the actin-binding protein within the muscle tissue (Figure 3).

Fig.2

Fig.3

Since many applications of tissue array technology rely on nucleic acid assays, we used the Complete View male mouse validation array slides for an in situ hybridization experiment (Figure 4). One of two slides was treated with RNase prior to hybridization of both slides with a fluorescently labeled poly(dT) probe. Salivary gland tissue on the slide without RNase digestion clearly shows the presence of mRNA . We used standard conditions for slide preparation, prehybridization, and hybridization reactions (Methods).

Fig.4

Conclusions

The Complete View tissue array slide kits permit wide- spread use of tissue array technology. With the single-slide format and convenient configuration of tissues, these innovative array slides simplify screening procedures so specialized expertise in histology is not required. We demonstrated their use to localize expression to specific tissues and cells, and our data confirm that these optimized array slides maintain the morphological integrity of tissues. By immunostaining of the tropomyosin protein and fluorescent labeling of RNA, we verified that the tissues affixed to the Complete View slides retain antigenicity of proteins and reactivity of nucleic acids, respectively. We are expanding this product line to include human fetal validation and survey arrays.

Methods

In Situ Hybridization: Paraffin was removed from the array tissue by treating the slides as described in the product instructions. The two consecutive slides
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
2. Simple Isolation of RNA from Tissue and Cultured Cells
3. Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer
4. Hands-free Tissue Disruption No Homogenization Needed
5. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
6. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
7. Stabilizing RNA in Fresh and Frozen Tissues for RNA Isolation
8. Genomic DNA Preparation from RNAlater Preserved Tissues
9. DNA Isolation From 300 to 450 mg Dried or 600 to 1,200 mg Fresh Leaf Tissue
10. DNA Isolation From 50 to 20 mg of Paraffin-Embedded Tissue
11. Genomic DNA Isolation From 510 mg Marine Invertebrate Tissue
Post Your Comments:
(Date:11/27/2014)... Nov. 27, 2014 Research and ... "Global Palmitic Acid Industry Report 2014" report to ... The Global Palmitic Acid Industry Report 2014 ... state of the global palmitic acid industry. ... including definitions, classifications, applications and industry chain structure. The ...
(Date:11/27/2014)... November 26, 2014 Biomedical devices that ... systems to enable 3D manufacture at the micro-nanoscale, and ... are among finalists in nine categories for the ... are sponsored by SPIE, the international society for ... , Winners will be announced by industry leaders on ...
(Date:11/26/2014)... November 26, 2014 The North ... segments the market for detailed analysis of the ... market to reach $616.3 million by 2018, growing ... 2018. , Browse through the TOC of the ... get an idea of the in-depth analysis and ...
(Date:11/26/2014)... , Nov. 26, 2014 The ... results from a survey of European physicians at ... on Innovation and Biological Therapies" at the Spanish ... event, hosted by EuropaBio and the Spanish Bioindustry ... of Health, physicians from Spanish oncology and rheumatology ...
Breaking Biology Technology:Global Palmitic Acid Industry Report 2014 2Finalists Named for 2015 Prism Awards for Photonics Innovation 2Finalists Named for 2015 Prism Awards for Photonics Innovation 3Finalists Named for 2015 Prism Awards for Photonics Innovation 4Finalists Named for 2015 Prism Awards for Photonics Innovation 5The North America Thermal Protective Clothing Market is estimated to grow up to $616.3 million by 2018 - Report by MicroMarket Monitor 2The North America Thermal Protective Clothing Market is estimated to grow up to $616.3 million by 2018 - Report by MicroMarket Monitor 3ASBM Presents European Physicians Survey at Spanish Ministry of Health 2
... hit universities and colleges across the country, including ... copyright infringement of both movies and music over ... several cease-and-desist notices to the Universirty of Wisconsin- ... were hosting movie downloads with content that belonged ...
... the information age similar to what the business world ... productivity of our staff, reduce costs, and improve our ... considerable engineering effort that requires hospital officials to look ... can embrace the changes that will improve healthcare. , ...
... Wisconsin has been a leader both in cutting-edge health ... nations leading research universities in UW-Madison, and leading-edge medical ... poised to lead again as the two fields merge ... engineering have long contributed to health care by providing ...
Cached Biology Technology:File Swapping: Download at your own Risk 2File Swapping: Download at your own Risk 3Im Still Waiting for the Information Age in Healthcare 2Im Still Waiting for the Information Age in Healthcare 3The Role of Engineering in Health Science and Technology 2The Role of Engineering in Health Science and Technology 3
(Date:11/3/2014)... low birth weight and preterm birth are linked to ... Findings published in the American College of Rheumatology (ACR) ... low birth weight and pre-term babies were not at ... adults. , According to the ACR, 27 million ... clinical OA. Symptoms of OA range from mild to ...
(Date:11/2/2014)... ORLEANS, LA (2 November 2014)—In support of a bold ... Bill & Melinda Gates Foundation today announced an award ... Malaria Vaccine Initiative (MVI) in building new vaccines that ... help realize the "accelerating to zero" agenda. Such vaccines ... what could be called an "immunological bed net." ...
(Date:11/2/2014)... into a bar and bump into a biologist . . ... some late-night nerd sketch, researchers have taken this premise and ... namely, finding meaning in the rising oceans of genomic data. ... mutations that genome-wide studies are publishing at a dizzying rate. ... the signal from the noise (and there is no shortage ...
Breaking Biology News(10 mins):Preterm, low birth-weight babies may need new hips in adulthood 2New malaria vaccines to prevent infection and block transmission get a shot in the arm 2New malaria vaccines to prevent infection and block transmission get a shot in the arm 3New malaria vaccines to prevent infection and block transmission get a shot in the arm 4Mutant models 2Mutant models 3Mutant models 4
... provide statin drugs free of charge so that customers can ... Imperial College London suggest in a new study published this ... in the blood. A wealth of trial data has proven ... attack risk. In a paper published in the Sunday ...
... Mount Sinai School of Medicine, New York, has been ... receive a 2010 ICAAC Young Investigator Award. Sponsored ... an early career scientist for research excellence in microbiology ... postdoctoral training in Molecular Biology at Harvard University in ...
... 250 scientists, engineers, government advisers and groups concerned ... from August 15-20, 2010 to consider the impact ... University of Maryland is co-sponsoring the conference, The ... ), which is co-organized by University of Maryland ...
Cached Biology News:Free statins with fast food could neutralize heart risk, scientists say 2University of Maryland co-sponsors International Conference on the Effects of Noise on Aquatic Life 2University of Maryland co-sponsors International Conference on the Effects of Noise on Aquatic Life 3
... in-house demand is causing shortages in your ... This can cause unwanted distractions, problems and ... royalties for your hard work and endeavour! ... build it up,and market it internationally. We ...
Topoisomerase I...
...
... virus is a type I eukaryotic topoisomerase ... turns (also called right- and left-handed supercoils) ... the reaction is a covalently closed, circular ... turns. DNA Topoisomerase I does not absolutely ...
Biology Products: