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Innovative Tissue Array Technology for High-Throughput Screening of Gene,,,Expression

sues and cell types in an animal species are screened. Traditional sections for whole-animal surveys require approximately 25 to 40 histological slides for complete screening. With each slide requiring preparation and processing that includes staining, labeling, and analysis, such surveys are so demanding of time, labor, and reagents that they are impractical in most research environments. In contrast, a single Complete View tissue array can contain all the major tissues and cell types of an animal. Hence, tissue array technology makes it possible to screen numerous tissues in a single experiment (Figure 1).

Fig.1

Stratagene has improved tissue array technology by creating the Complete View survey and validation array slides. In this product line, optimized tissue fixation preserves morphological integrity, antigenicity of proteins, and nucleic acid reactivity. On each Complete View array slide, tissues are conveniently configured for easy identification with the Complete View target locator, which positions each tissue within the array by column and row. The availability of two categories of array slides provides further convenience. The Complete View survey array slides represent major organ systems that consist of approximately 104 cell types mounted in duplicate (Table 1). The Complete View validation array slides include representative tissues from the survey arrays and are useful to test staining procedures and reagents.

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