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Innovative Tissue Array Technology for High-Throughput Screening of Gene,,,Expression


Many different tissue types on a convenient single-slide format

Carolyn Thomas
Stratagene

Stratagenes Complete View tissue array slides offer further refinement of tissue array technology through optimized tissue fixation and processing procedures that preserve tissue morphology, antigenicity of proteins, and nucleic acid reactivity. This innovative product line consists of murine (male and female) survey and validation array slides. The Complete View survey array slides represent major organ systems, consisting of approximately 104 cell types mounted in duplicate. The Complete View validation array slides include 12 representative tissues from the survey arrays and are useful to test staining procedures and reagents. We report our results using Complete View array slides for hematoxylin and eosin staining of murine tissue, immunostaining of murine tropomyosin, and labeling RNA of murine salivary gland. These tests confirm that Complete View tissue array slides ensure reliable screening results in a convenient, high-throughput format.

To achieve an understanding of the cellular function of gene products requires localizing gene expression to specific cells and tissues. This task can be accomplished through the use of tissue arrays, a collection of diverse tissue samples mounted on a single slide. Proven successful in examining both normal1,2 and cancerous tissues,3,4 tissue arrays can be treated as a single histological slide during staining, immunohistochemistry, or in situ hybridization. The single-slide format affords a high-throughput method for precisely identifying the specific tissues and cell types that express a gene of interest.

Single-Slide Whole-Animal Surveys

One particularly useful application of tissue arrays is the whole-animal survey, in which all tis
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