Navigation Links
Inducing RNAi with siRNA Cocktails Generated by RNase III

Mike W. Byrom, Angie M. Cheng and Lance P. Ford*
Ambion, Inc.
2130 Woodward Street
Austin, TX 78744-1832
*Corresponding author email: lford@ambion.com


Abstract
Small interfering RNA (siRNA) is an extremely effective tool for reducing gene expression in a variety of systems, including mammalian cells. Gene specific siRNAs have typically been made by chemical synthesis. Producing them by chemical synthesis, however, is costly, and while most siRNAs exhibit some effect, only about 25% of those designed reduce target levels by 80% or more (1). A new in vitro procedure that cleaves long dsRNA into a mixture of siRNAs significantly reduces the cost of siRNA preparation. The resulting siRNA population is effective at mediating gene specific silencing and bypasses the need to screen for functional siRNAs.


Introduction
In the RNA interference (RNAi) pathway in C. elegans, Drosophila, and other diverse organisms, long double-stranded RNA (dsRNA) introduced into a cell is cleaved into a mixture of siRNAs by an RNase III-like endoribonuclease (Dicer) producing siRNAs that are 21-23 bp in length and contain 3' dinucleotide overhangs with 5'-phosphate and 3'-hydroxyl termini. These siRNAs mediate degradation of homologous transcripts, leading to gene silencing (2-5; see also "Gene Specifi
'"/>


Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Custom and library siRNA for efficient gene silencing
2. Custom and library siRNA for efficient gene silencing
3. Cancer siRNA Oligo Set Version 1.0
4. Library siRNA
5. Custom siRNA Oligo Synthesis Service
6. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
9. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
10. siRNA transfection optimization with the Agilent 2100 bioanalyzer
11. siRNA Design Guidelines
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Inducing RNAi with siRNA Cocktails Generated RNase III

(Date:8/27/2014)... --  MSC , a healthcare performance improvement company advancing ... of Mary Beth Loesch to President and ... preparing companies for rapid growth and market expansion, Loesch ... Corporate Development and Healthcare. In that role, she oversaw ... as corporate strategy and marketing. Previously, Loesch served as ...
(Date:8/27/2014)... YORK , Aug. 27, 2014 ... is available in its catalogue: Global ... http://www.reportlinker.com/p02316662/Global-Chelating-Agents-Market-2014-2018.html About Chelating Agent A ... form covalent bonds with metal ions, thereby forming ... that some metal-ions have on chemical processes, formulations, ...
(Date:8/27/2014)... , Aug. 27, 2014  A novel ... to help physicians better identify early-stage disease, when ... disability, according to a new study in The ... Diagnostics (NYSE: DGX ) and other ... 14-3-3eta protein outperformed conventional antibody-serum testing, including rheumatoid ...
(Date:8/27/2014)... -- Reportbuyer.com has added a new market research ... Rational View https://www.reportbuyer.com/product/1595336/The-Graphene-Opportunity---A-Rational-View.html ... similar amount of hype currently surrounds graphene, with ... water treatment, but is graphene the only solution? ... Opportunity Report, authored by Tim Harper ...
Breaking Biology Technology:MSC names Mary Beth Loesch President and CEO 2Global Chelating Agents Market 2014-2018 2Global Chelating Agents Market 2014-2018 3Global Chelating Agents Market 2014-2018 4Novel Biomarker Detects Early Rheumatoid Arthritis Better than Conventional Methods Alone, According to Journal of Rheumatology Study 2Novel Biomarker Detects Early Rheumatoid Arthritis Better than Conventional Methods Alone, According to Journal of Rheumatology Study 3Novel Biomarker Detects Early Rheumatoid Arthritis Better than Conventional Methods Alone, According to Journal of Rheumatology Study 4The Graphene Opportunity - A Rational View 2The Graphene Opportunity - A Rational View 3The Graphene Opportunity - A Rational View 4
... 2012 The National Foundation for Cancer Research announced ... have been awarded the 7th annual Szent-Gyorgyi Prize for ... led to the successful development of a new therapeutic ... By combining traditional Chinese medicine with Western medicine, Drs. ...
... University of Pennsylvania research team have shown that they can ... Pigmentosa, or XLRP, in dogs. The disease in humans ... and results in early, severe and progressive vision loss. It ... degeneration in man. "Every single abnormal feature that ...
... is largely transparent to the eye and, as it turns ... by scientists at Rice University and Rensselaer Polytechnic Institute (RPI) ... wet when clad by a single continuous layer of graphene ... in the online edition of Nature Materials , is ...
Cached Biology Technology:Chinese Scientists Zhen-Yi Wang and Zhu Chen Awarded 7th Annual Szent-Gyorgyi Prize for Progress in Cancer Research 2Chinese Scientists Zhen-Yi Wang and Zhu Chen Awarded 7th Annual Szent-Gyorgyi Prize for Progress in Cancer Research 3Chinese Scientists Zhen-Yi Wang and Zhu Chen Awarded 7th Annual Szent-Gyorgyi Prize for Progress in Cancer Research 4Chinese Scientists Zhen-Yi Wang and Zhu Chen Awarded 7th Annual Szent-Gyorgyi Prize for Progress in Cancer Research 5Gene therapy research from Penn Vet & Scheie Eye Inst. cures retinitis pigmentosa in dogs 2Gene therapy research from Penn Vet & Scheie Eye Inst. cures retinitis pigmentosa in dogs 3Water sees right through graphene 2
(Date:8/27/2014)... finds , Gamblers show the same tendencies as ... shown. , Researchers, led by Dr Elliot Ludvig of ... that found that both human gamblers and pigeons were ... rewards. , Published in Biology Letters , the ... role that memories of previous biggest wins and losses ...
(Date:8/26/2014)... University in St. Louis have developed algorithms to identify ... tearing or breaking. The technology, which needs to be ... may help pinpoint minor strains and tiny injuries in ... The research is available online Aug. 27 in the ... publishes research at the nexus of the physical and ...
(Date:8/26/2014)... the University of Warwick have provided the first ... protein is linked to early signs of dementia. ... research found that the absence of the protein ... in the nervous system. These changes were shown ... of dementia, including restricted learning and memory formation ...
Breaking Biology News(10 mins):Gamblers are greedy bird-brains, University of Warwick research finds 2New technology may identify tiny strains in body tissues before injuries occur 2New technology may identify tiny strains in body tissues before injuries occur 3Lack of naturally occuring protein linked to dementia 2
... a daunting task to find a smoking gun for ... say not just when and how a specific gene ... into real-world dominance of one population over another. , ... bacteria to compete in a head-to-head contest for evolutionary ...
... enzyme found in the venom of the brown recluse spider, ... discovered a new way to open molecular pores, called ion ... Lu, MD, PhD; Yajamana Ramu, PhD; and Yanping Xu, MD, ... from over 100 poisonous invertebrate species to make this discovery. ...
... researchers at the University of Illinois at Urbana-Champaign have observed ... major role in repairing damaged DNA. , The protein forms ... at a time, the researchers report in the August issue ... protein found in the gut bacterium E. coli. A human ...
Cached Biology News:Ready, set, mutate?and may the best microbe win 2Ready, set, mutate?and may the best microbe win 3Gatekeeping: Penn researchers find new way to open ion channels in cell membranes 2Recombination protein dynamics observed with single monomer resolution 2
Mouse Anti-Human Kallikrein 2 (Hk2)...
... (Ankyrin repeat and SOCS box-containing protein) This ... containing amino acids 75-93 and 348-368 of ... Catalog No. 40149) can be used as ... with mouse and rat Asb-2, based on ...
Recognizes TWIK-2. Does not cross react with any other known proteins....
... Glycine Transporter 2, Neuronal (GLYT2). The ... from the central nervous system. The staining ... pattern described using in situ hybridization with ... has been described for glycinergic neurons. Preabsorption ...
Biology Products: