Navigation Links
Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell

transfer because the buffer reservoirs, the filter paper on both sides of the gel, are independent. In a discontinuous system, methanol should be included in the buffer on the membrane side (anode) of the blot assembly and SDS used on the gel side (cathode), taking advantage of the positive effects of each component.

A new discontinuous buffer system using a Tris-CAPS buffer provides excellent results in semi-dry blotting. This new procedure uses Tris-CAPS buffer plus 15% methanol in the filter paper on the anode and Tris-CAPS plus 0.1% SDS in the filter paper on the cathode.

Cytosol from white blood cells was separated by SDS-PAGE. The phox-47 component of the Respiratory Burst Oxidase was detected by Immun-Star chemiluminescent substrate.


For Best Results
Bio-Rads Extra Thick Blotting Paper should be used. This paper is 2.6 mm thick, 100% cotton fiber, and provides the absorbency required for semi-dry blotting.

PVDF provides superior retention of the protein. For proteins less than 20 K it is required to prevent blow-through during electrophoretic transfer. Its higher binding capacity will increase detection results. It is important to first wet the PVDF in 100% methanol, then equilibrate in bottom/anode buffer for at least 30 minutes prior to transfer. It is expedient to begin equilibration during the vertical electrophoresis run.

Buffer Formulation
Tris-CAPS (60 mM Tris base, 40 mM CAPS, pH 9.6)
5x Stock Solution
36.34 g Tris-base
44.26 g CAPS
Water to 1 liter

To prepare 100 ml working buffers from 5x stock solution:
Bottom/Anode buffer: Top/Cathode bu
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Increased Sensitivity in Microarray Analysis
2. Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery
3. DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell
4. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
5. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
6. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
7. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
8. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
9. Enhancing PCR* Efficiency with MasterTaq
10. Quantification of siRNA Silencing Efficiency Using the LightCycler System
11. A New Way to Assess siRNA Delivery Efficiency
Post Your Comments:
(Date:7/10/2014)... Poway, CA (PRWEB) July 10, 2014 Robert ... Regenerative Veterinary Medicine company, is proud to announce the relaunch ... Pets, launching with a new series called “ What are ... to give an honest and straightforward foundation in the basics ... an educated decision on the right type of treatment when ...
(Date:7/10/2014)... CROWN POINT, Ind. , July 10, ... recently expanded its use of capnography for ... a growing group of healthcare leaders in ... Capnography measures how effectively patients are breathing ... respiratory depression occurs. By measuring the amount ...
(Date:7/10/2014)... Research and Markets ( http://www.researchandmarkets.com/research/s3bqj2/photonic ) ... Photonic Integrated Circuit (Monolithic Integration, Hybrid Integration, Module ... to their offering. ... integration traces its roots in the latter half ... integration went unexplored and unfulfilled for several decades ...
(Date:7/10/2014)... Using microscopic polymer light resonators that expand in ... Quantum Photonics Laboratory have developed new optical sensors ... Optical sensors are ideal for detecting trace gas ... lightweight nature, and immunity to electromagnetic interference. , ... before, the MIT team conceived an extremely sensitive, ...
Breaking Biology Technology:Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3Franciscan St. Anthony Health-Crown Point Underscores Patient Safety Commitment Through Expanded Use of Capnography 2International Photonic Integrated Circuit (Monolithic Integration, Hybrid Integration, Module Integration) Market - Forecasts to 2019 2Swell new sensors 2
... ISELIN, N.J., July 30 SyntheMed, Inc. (OTC ... development and commercialization of anti-adhesion products, announced today that ... its bioresorbable reverse thermal gelation polymer technology. This ... this novel technology and pertains to the application of ...
... , MONMOUTH JUNCTION, N.J., July ... into a cooperative research and development agreement with the National ... National Institutes of Health (NIH), to study ARIKACE(TM) (liposomal amikacin ... , , Transave and NIAID will collaborate ...
... , CHADDS FORD, Pa., July 30 Endo Pharmaceuticals ... quarter of 2009, including a full quarter of results of Indevus Pharmaceuticals, ... Total revenues during the second quarter of 2009 increased 22 percent ... 2008. Net income for the three months ended June 30, 2009, ...
Cached Biology Technology:SyntheMed Receives US Patent on Novel Polymer Technology 2Transave Enters Into Cooperative Research Agreement With NIH To Study ARIKACE(TM) In Nontuberculous Mycobacteria 2Transave Enters Into Cooperative Research Agreement With NIH To Study ARIKACE(TM) In Nontuberculous Mycobacteria 3Transave Enters Into Cooperative Research Agreement With NIH To Study ARIKACE(TM) In Nontuberculous Mycobacteria 4Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 2Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 3Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 4Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 5Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 6Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 7Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 8Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 9Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 10Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 11Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 12Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 13Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 14Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 15Endo Pharmaceuticals Reports Strong Second Quarter 2009 Financial Results 16
(Date:7/11/2014)... July 10, 2014---A team of researchers from The Chinese ... identified a gene of wild soybean linked to salt ... grow in saline soil. This study published online in ... unveil novel genomic information for crop improvement. , Soybean ... domestication and human selection, cultivated soybeans have less genetic ...
(Date:7/11/2014)... July 11, 2014 Researchers have pioneered ... images. The new technology, called Virtual Finger, allows ... structures like neurons and synapses using the flat ... technology makes 3D imaging studies orders of magnitude ... an unprecedented level across many areas of experimental ...
(Date:7/11/2014)... receptor type 1 can inhibit voltage-gated calcium ... neurotransmitter release. However, some scholars demonstrated that ... Ca2+ influx and increase neurotransmitter release. Dr. ... Affiliated to Tongji Medical College, Huazhong University ... cell voltage-clamp and calcium imaging in cultured ...
Breaking Biology News(10 mins):BGI reports a novel gene for salt tolerance found in wild soybean 2Virtual finger enables scientists to navigate and analyze complex 3D images 2
... MURCIA, SPAINSalt can have drastic effects on the ... that salinity renders an about one-third of the world,s ... salinity can cause ion toxicity, osmotic stress, mineral deficiencies, ... stress can even cause plants to adjust their water ...
... of modern humans like more than 50,000 years ago? ... Stone Age engravings are the subjects of a good deal ... journal Science , an international team of researchers report ... could place the use of herbal medicines much earlier than ...
... growing in popularity as more growers turn to strip-till. However, ... worth. A new University of Illinois study revealed ... soybean. However, the results showed no difference in yield between ... corn, not soybean," said Fabian Fernandez, U of I assistant ...
Cached Biology News:Salt-tolerant crops show higher capacity for carbon fixation 2Earliest known bug-repellant plant bedding found at South African rock shelter 2Earliest known bug-repellant plant bedding found at South African rock shelter 3Earliest known bug-repellant plant bedding found at South African rock shelter 4Strip-till improves nutrient uptake and yield 2
SHEEP ANTI BOVINE LACTOPEROXIDASE...
... in utility and flexibility press-to-seal silicone isolators ... of the researcher. They are available either ... sheet material that can be easily trimmed ... autoclavable and non-cytotoxic and can be used ...
Request Info...
... Provides up to 30 minutes of walkaway time. Automatic Calibration. Five minute cooldown ... port. ... ... ...
Biology Products: