Navigation Links
Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell

transfer because the buffer reservoirs, the filter paper on both sides of the gel, are independent. In a discontinuous system, methanol should be included in the buffer on the membrane side (anode) of the blot assembly and SDS used on the gel side (cathode), taking advantage of the positive effects of each component.

A new discontinuous buffer system using a Tris-CAPS buffer provides excellent results in semi-dry blotting. This new procedure uses Tris-CAPS buffer plus 15% methanol in the filter paper on the anode and Tris-CAPS plus 0.1% SDS in the filter paper on the cathode.

Cytosol from white blood cells was separated by SDS-PAGE. The phox-47 component of the Respiratory Burst Oxidase was detected by Immun-Star chemiluminescent substrate.


For Best Results
Bio-Rads Extra Thick Blotting Paper should be used. This paper is 2.6 mm thick, 100% cotton fiber, and provides the absorbency required for semi-dry blotting.

PVDF provides superior retention of the protein. For proteins less than 20 K it is required to prevent blow-through during electrophoretic transfer. Its higher binding capacity will increase detection results. It is important to first wet the PVDF in 100% methanol, then equilibrate in bottom/anode buffer for at least 30 minutes prior to transfer. It is expedient to begin equilibration during the vertical electrophoresis run.

Buffer Formulation
Tris-CAPS (60 mM Tris base, 40 mM CAPS, pH 9.6)
5x Stock Solution
36.34 g Tris-base
44.26 g CAPS
Water to 1 liter

To prepare 100 ml working buffers from 5x stock solution:
Bottom/Anode buffer: Top/Cathode bu
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Increased Sensitivity in Microarray Analysis
2. Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery
3. DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell
4. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
5. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
6. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
7. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
8. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
9. Enhancing PCR* Efficiency with MasterTaq
10. Quantification of siRNA Silencing Efficiency Using the LightCycler System
11. A New Way to Assess siRNA Delivery Efficiency
Post Your Comments:
(Date:7/30/2014)... July 30, 2014 Yuma Regional ... and complementary therapies brings comprehensive cancer care to ... Yuma Regional Cancer Center has officially opened. A ... program includes services such as open, semi-private and ... American Cancer Society Resource Center, hematology, rehabilitation center, ...
(Date:7/30/2014)... Md. , July 30, 2014 ... components to interrogate biology, but such devices can ... As such, researchers have looked ... in vitro metabolic engineering to characterize ... a chip. New devices are envisioned to recreate ...
(Date:7/30/2014)... , July 30, 2014  RXi Pharmaceuticals ... biotechnology company focused on discovering, developing and ... needs using RNA-targeted technologies, today announced that ... RXI-109, for the reduction of recurrence of ... has been initiated. Logo - ...
(Date:7/30/2014)... San Diego, CA (PRWEB) July 30, 2014 ... second contract from the U.S. Environmental Protection Agency ... company’s expertise in developmental toxicity models, including tests ... on neuron formation and the formation of connections ... function. Inhibition of neurodifferentiation and synapse formation by ...
Breaking Biology Technology:Sate-of-the-Art Yuma Regional Cancer Center Officially Opens 2UMD Researchers Bridge Gap between Microelectronics, Biological Systems 2UMD Researchers Bridge Gap between Microelectronics, Biological Systems 3UMD Researchers Bridge Gap between Microelectronics, Biological Systems 4RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 2RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 3RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 4RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 5U.S. Environmental Protection Agency (EPA) Awards Vala Sciences a Second ToxCast Contract 2U.S. Environmental Protection Agency (EPA) Awards Vala Sciences a Second ToxCast Contract 3
... in Series of Global Distribution Agreements to Make ... Available to Researchers Everywhere--ZyGEM Also Announces Issuance of ... SOLANA BEACH, Calif. and HAMILTON, New Zealand ... Corp. Ltd. today announced that it has entered ...
... May 28 GenVec, Inc. (Nasdaq: GNVC ... with a single institutional investor for the sale of 9,615,385 shares ... its common stock. The shares of common stock and warrants ... stock and a warrant to purchase one share of common stock ...
... the Chinese Academy of Sciences in Beijing, scientists have ... to understand how they may help in the development ... , The research focused on rotating magnetic fields, which ... and generators. The difficulty for technology at the atomic ...
Cached Biology Technology:ZyGEM Corp. Signs Agreement to Incorporate Its Breakthrough DNA Extraction Technology Into Rubicon's Research and Diagnostic Products 2ZyGEM Corp. Signs Agreement to Incorporate Its Breakthrough DNA Extraction Technology Into Rubicon's Research and Diagnostic Products 3ZyGEM Corp. Signs Agreement to Incorporate Its Breakthrough DNA Extraction Technology Into Rubicon's Research and Diagnostic Products 4GenVec Announces Registered Offering of $6 Million in Common Stock and Warrants 2GenVec Announces Registered Offering of $6 Million in Common Stock and Warrants 3
(Date:7/30/2014)... baboons suggests that the route to a higher rank is ... of supportive sisters. , A female baboon,s social status is ... of her mother - the higher the mother is ranked, ... rank in female baboons is thought to be determined at ... spot in the pecking order, whereas females born to low-ranking ...
(Date:7/30/2014)... 30, 2014 NexID Biometrics , ... detection solutions for the biometric authentication industry, has ... revolutionizing online authentication with standards for strong authentication. ... "The decision to join FIDO Alliance stems from ... in use. We believe the FIDO Alliance is ...
(Date:7/30/2014)... through a massive collection of 20-million-year-old amber found in ... the effort is yielding fresh insights into ancient tropical ... collection is fully curated, a task that will take ... amber collection in the world, the researchers report. ... that of a pygmy locust, a tiny grasshopper the ...
Breaking Biology News(10 mins):Supportive moms and sisters boost female baboon's rank 2Supportive moms and sisters boost female baboon's rank 3NexID Biometrics Joins the FIDO Alliance 2Decades-old amber collection offers new views of a lost world 2
... salmon make one heck of a commute. The record holder in ... in the Clearwater River, Idaho, in April 2003. A year and ... near Japan. The most direct route between those two points ... Imagine fish that make it that far then turn around and ...
... reduces illness severity and duration in children one ... hours of symptom onset, according to new data ... Disease (WISPID) meeting in Bangkok 15-18 November. The ... incidence of respiratory tract infections, ear infections and ...
... as one-third of global energy consumption. However, unconventional changes ... emissions. ETH Zurich Professor for Sociology, Dirk Helbing, has ... could improve vehicular traffic flow by up to 95 ... ETH Zurich Chair of Sociology, a specialist in modelling ...
Cached Biology News:UW launches cutting-edge DNA 'fin-printing' project for salmon 2UW launches cutting-edge DNA 'fin-printing' project for salmon 3Tamiflu effective for treatment and prevention of influenza in children 1 year and older 2Tamiflu effective for treatment and prevention of influenza in children 1 year and older 3Traffic control systems 2Traffic control systems 3Traffic control systems 4
Taq DNA Polymerase (cloned), 250 units. Category: Nucleotides & Enzymes & Biochemicals, Modifying Enzymes, Polymerases....
Request Info...
Request Info...
Candida (IgA) -Ab EIA Sample Size: 5 l...
Biology Products: