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Increased Sensitivity in Microarray Analysis

A higher call rate and a larger number of uniquely detected genes are consistently observed for targets generated with the RAS Microarray Kits, indicating increased sensitivity.

Furthermore, correlation factors were calculated for a pair-wise comparison of the three chips hybridized with target replicates; only genes detected with all three target replicates were included in the calculation (Table 2). For chips hybridized with targets that were generated using the RAS Micorarray Kit workflow, correlation is very good (> 0.99) for all pair-wise comparisons. When targets were prepared with Supplier As workflow the correlation is somewhat lower. The good correlation observed for chips hybridized with targets synthesized with the RAS Microarray Kits demonstrates the high reproducibility of the RAS target preparation procedure.

In summary, our data show that the RAS Microarray Kit workflow for target preparation consistently leads to very sensitive and highly reproducible microarray results.




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