Navigation Links
Increased Sensitivity in Microarray Analysis

ysis was performed using the Affymetrix platform- specific software (present calls, signal intensity values) and MS Excel for calculation of the correlation factors (r2).

Data Analysis
We first analyzed the present call percentage. The number of present calls equals the number of genes (probe sets) that are detectable on a chip. This number corresponds to the genes being expressed in the particular sample.



A significant increase in the number of present calls was observed for both samples when labeled targets were prepared with the RAS Microarray Kits as shown in Table 1.



Figure 2 shows the distribution of the present calls in more detail. The genes analyzed are those that were detected with all three target replicates, prepared by the indicated workflow. For sample 1, 10,339 genes were found to be present when targets were prepared with the RAS Microarray Kits. Of these, 9,331 were also detected with all three targets prepared by Supplier As workflow.



For sample 2, when targets were prepared with the RAS Microarray Kits, 9,652 genes were detected. 7,887 of these genes were also detected with all three targets when Supplier As workflow had been used.

Using the RAS Microarray Kit workflow for target preparation, we identified 185 genes for sample 1 and 283 genes for sample 2 that could not be detected with any replicate when targets where prepared by Supplier As workflow. Vice versa, only 63 genes for sample 1 and 21 genes for sample 2 were not detected with targets prepared with the RAS Microarray Kits


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell
2. Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery
3. Improved Sensitivity for Staining RNA with SYBR Gold Stain
4. Enhancing Ruggedness and Full-Scan MS Sensitivity Using Ion Sweep Technology
5. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
6. Femtogram Sensitivity of the Antipsychotic Drugs Raclopride and Ritanserin Using a Varian 1200L LC/MS/MS
7. Maintaining High Sensitivity when Working with Small-Volume Samples
8. High Sensitivity, Wide Dynamic Range, Horseradish Peroxidase (HRP) ELISA Using the LMax Microplate Luminometer (MaxLine Application Note #41)
9. Sensitivity and Quantitation using a Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer
10. Trace Analysis Optimizing Sensitivity Finnigan Surveyor PDA Detector
11. A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Increased Sensitivity Microarray Analysis

(Date:5/28/2015)... -- Quest Diagnostics Incorporated (NYSE: DGX ), the world,s ... is scheduled to speak at the Jefferies 2015 Global ... the company,s President and CEO, will discuss the company,s ... scheduled for Tuesday, June 2, 2015 at 8:00 a.m. ... live during the conference and will be available to ...
(Date:5/28/2015)... Research and Markets( http://www.researchandmarkets.com/research/6cg3zw/cell_therapy ) has ... "Cell Therapy - Technologies, Markets and Companies" ... describes and evaluates cell therapy technologies and methods, ... role in the practice of medicine. Hematopoietic stem ... marrow transplants. Role of cells in drug discovery ...
(Date:5/28/2015)... CARLOS, Calif. , May 28, 2015 /PRNewswire/ ... testing, today announced a study published in ... the Panorama single-nucleotide polymorphism (SNP)-based non-invasive prenatal test ... the first trimester. A study ... that complete molar pregnancies occur in about 1 ...
(Date:5/28/2015)... Mass. , May 28, 2015 RXi ... focused on discovering and developing innovative therapeutics, primarily in ... pricing of a public offering of 26 million units ... share for gross proceeds of approximately $10.4 million. Each ... (ii) a 13-month overallotment purchase right to purchase 0.50 ...
Breaking Biology Technology:Quest Diagnostics to Speak at the Jefferies 2015 Global Healthcare Conference 2Global Cell Therapy Market 2015-2020 - Technologies, Markets and Companies 2First Confirmed Detection of a Complete Molar Pregnancy with Cell-Free DNA Test 2First Confirmed Detection of a Complete Molar Pregnancy with Cell-Free DNA Test 3RXi Pharmaceuticals Announces $10 Million Public Offering of Common Stock and Warrants 2RXi Pharmaceuticals Announces $10 Million Public Offering of Common Stock and Warrants 3
... HUBBARD, Ohio, Jan. 12 NanoLogix, Inc. (OTC: NNLX) ... update. NanoLogix welcomes Daniel Barnhizer and Jeff Long to ... professor of law at Michigan State University ... the areas of business, contract, securities, and commercial law. ...
... PARIS, January 12 The French agency Agence ... Dr. Kamel Senouci as,Program Director for the SIVAC ... program aims at contributing to,the development of national ... in Africa and six in Asia. These committees ...
... PARK, Ill. and SANTA ANA, Calif., Jan. 12 ,Abbott ... EYE ) announced,today a definitive agreement for Abbott ... total transaction value of approximately $2.8 billion, inclusive,of estimated net ... Ana, Calif., AMO,is a global leader in ophthalmic care, comprised ...
Cached Biology Technology:NanoLogix Inc. Appoints Two New Directors and Provides Operations Update 2NanoLogix Inc. Appoints Two New Directors and Provides Operations Update 3French Agency AMP Appoints Dr Kamel Senouci as Director of SIVAC Initiative (Supporting Independent Immunization and Vaccine Advisory Committees) 2French Agency AMP Appoints Dr Kamel Senouci as Director of SIVAC Initiative (Supporting Independent Immunization and Vaccine Advisory Committees) 3Abbott Expands Its Growing Medical Device Business With Acquisition of Advanced Medical Optics (AMO) 2Abbott Expands Its Growing Medical Device Business With Acquisition of Advanced Medical Optics (AMO) 3Abbott Expands Its Growing Medical Device Business With Acquisition of Advanced Medical Optics (AMO) 4Abbott Expands Its Growing Medical Device Business With Acquisition of Advanced Medical Optics (AMO) 5Abbott Expands Its Growing Medical Device Business With Acquisition of Advanced Medical Optics (AMO) 6Abbott Expands Its Growing Medical Device Business With Acquisition of Advanced Medical Optics (AMO) 7
(Date:4/27/2015)... FORT LAUDERDALE, Fla. , Apr. 27, 2015 /PRNewswire/ ... provider of access control and security systems is pleased ... joined the company as a consultant and member of ... Bedore is widely known as a thought leader ... Socrates Distance Learning Technology Group, and has published studies ...
(Date:4/20/2015)... The announcement comes as demand for fire ... Ireland,s foremost records management company, has ... an impressive track record of clients within the first 12 ... management sector in Dubai . As a ... employ a further eight staff members at its Irish headquarters. ...
(Date:4/14/2015)... April 14, 2015 NXT-ID, Inc. (NASDAQ: ... company focused on the growing mobile commerce market, announces ... the 2015 New York Design Awards under the category  ... York City Design Awards are part of a global ... 2,500 ratings from the marketplace, industry and judging panel. ...
Breaking Biology News(10 mins):Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 2Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 3Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 4Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 2Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 3NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 2NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 3
... the First World War, a lethal virus known as ... United States, Europe and Asia in three convulsive waves. ... to 100 million victims, most of whom succumbed to ... died within days of the first symptoms. ...
... ANDS ) announced today that it will present ... Monday, February 16, 2009 at 1:00 p.m. PST (4:00 p.m. ... Laguna Niguel. Steve Worland, Ph.D., President and Chief Executive Officer ... clinical programs: ANA598 and ANA773 in hepatitis C and ANA773 ...
... in eight women in the United States will receive a ... the second leading cause of cancer-related death in women. Now ... treatment for each patient. "Our research shows that there ... getting any treatment at all, other than a lumpectomy," explains ...
Cached Biology News:Shades of 1918? New study compares avian flu with a notorious killer from the past 2Shades of 1918? New study compares avian flu with a notorious killer from the past 3Shades of 1918? New study compares avian flu with a notorious killer from the past 4Shades of 1918? New study compares avian flu with a notorious killer from the past 5Anadys Pharmaceuticals to Present at the Roth OC Growth Stock Conference 2New test for breast cancer will help guide treatment choices 2
... Detection System is intended for use in ... by an in-house validated method for in ... target of interest. • ... a hybridization capture assay designed to detect ...
... maximum packaging efficiencies of cDNA and genomic DNA ... a test insert with Novagen's l vector arms, ... 10^8 pfu/g ligated l control DNA) are typically ... use; simply add up to 5 g of ...
Gridded Cell Culture Petri Dish (20 mm molded in base), tissue-culture treated...
Chicken Anti-Human DNA-directed RNA Polymerase II 7.6 Kd Polypeptide (POLR2L Polyclonal AntibodyOther Sub-Family: not assigned-Other Applications: ELISA, Western Blot...
Biology Products: