Navigation Links
Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery

the SRM experiment by the enhanced mass-resolution feature.

The LC/ESI-SRM chromatograms obtained at unit mass-resolution for compound A in plasma at a concentration of 250 ng/mL, are shown in Figure 3. Background interference to the signal of compound A at this concentration is clearly evident in the chromatographic trace. A calculated signal to noise (S/N) ratio of 8 is determined for this peak (Figure 3). Hence, the LOQ for compound A at unit mass-resolution can be assumed to be of the order of 250 ng/mL.

When the same sample of compound A (conc. 250 ng/mL) was analyzed at enhanced mass-resolution, a much cleaner SRM peak was observed, as shown in Figure 4. This is a consequence of the removal of a considerable amount of isobaric chemical interference from the analyte through the improved mass selectivity at enhanced mass-resolution. In addition to a more uniform SRM peak, an improved S/N ratio of 31 was obtained for compound A at enhanced mass-resolution, relative to unit mass-resolution (Figures 3,4). The dramatic decrease in background noise at the higher mass-resolution setting is responsible for the significant improvement in S/N ratio, despite the loss of a factor of 3-4 in peak height/area for compound A (Figures 3,4). An increase in analyte sensitivity at enhanced mass-resolution, relative to unit, has been reported in previous LC/ESI-SRM and LC/APCI-SRM quantitative studies on the Finnigan TSQ Quantum Discovery.[2-6] Indeed, one of the prior quantitative studies was based on the monitoring of SRM transitions involving small molecule losses,[6] analogous to the transition used in this work (m/z 473456, loss of 17 u). Since small molecule losses through collisionally-induced dissociation processes are common-place and the potential for matrix interference to the analyte SRM is much greater, analysis at enhanced mass-resolution should be an extremely viable method for improved quantitative performance in
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Increased Sensitivity in Microarray Analysis
2. Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell
3. Improved Sensitivity for Staining RNA with SYBR Gold Stain
4. Enhancing Ruggedness and Full-Scan MS Sensitivity Using Ion Sweep Technology
5. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
6. Femtogram Sensitivity of the Antipsychotic Drugs Raclopride and Ritanserin Using a Varian 1200L LC/MS/MS
7. Maintaining High Sensitivity when Working with Small-Volume Samples
8. High Sensitivity, Wide Dynamic Range, Horseradish Peroxidase (HRP) ELISA Using the LMax Microplate Luminometer (MaxLine Application Note #41)
9. Sensitivity and Quantitation using a Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer
10. Trace Analysis Optimizing Sensitivity Finnigan Surveyor PDA Detector
11. A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity
Post Your Comments:
(Date:9/15/2014)... 15 septembre 2014 La deuxième édition ... (semaine internationale sur la sensibilisation au plasma) ... conjointe de la Plasma Protein Therapeutics Association ... , l,IPAW vise à : , ... la collecte du plasma-aphérèse , Reconnaître la ...
(Date:9/15/2014)... ANNAPOLIS, Md. , Sept. 15, 2014  The ... will be celebrated October 12-18. A joint initiative of ... its member companies , IPAW is designed to: ... collection , Recognize the contributions of plasma donors ... about lifesaving plasma protein therapies and rare diseases ...
(Date:9/14/2014)... UMBC’s Biotechnology Graduate Programs’ Virtual Information ... from 12:00 to 1:00 p.m. EDT. The session will ... practice-oriented instruction, designed for working professionals. Participants will also ... career in the biotechnology industry. , If interested in ... UMBC’s Biotechnology Graduate Programs, UMBC offers a variety of ...
(Date:9/14/2014)... particularly ingenious when it comes to survival strategies. They ... hostile environment, for example during treatment with antibiotics. A ... a protective slime capsule consisting of sugar chains and ... have for the first time created a detailed three-dimensional ... blocks cross the bacterial cell wall, a crucial step ...
Breaking Biology Technology:L' International Plasma Awareness Week célébrera les donneurs et braquera les projecteurs sur les maladies rares 2L' International Plasma Awareness Week célébrera les donneurs et braquera les projecteurs sur les maladies rares 3International Plasma Awareness Week To Celebrate Donors And Spotlight Rare Diseases 2International Plasma Awareness Week To Celebrate Donors And Spotlight Rare Diseases 3UMBC Biotechnology Graduate Programs’ Virtual Info Session, October 21, 2014 2New insights in survival strategies of bacteria 2
... , , BOULDER, Colo. and THOUSAND OAKS, Calif., Dec. 14 ... AMGN ) today announced that they entered into an agreement ... including ARRY-403, currently being tested in a Phase 1 clinical trial ... terms of the agreement, Array will receive an upfront payment of ...
... , , JUPITER, Fla., Dec. ... today that it has extended its collaboration with The Scripps ... to re-annotate the genome of Dyadic,s patented and proprietary C1 ... successful relationship between the two organizations which began in March ...
... , ROCKAWAY, N.J., Dec. 14 ... Evidence-Based Approach , published by the Infusion ... anthracycline extravasation.(1) Anthracyclines are a group of chemotherapy medications ... in the treatment of various types of cancer since the ...
Cached Biology Technology:Array BioPharma and Amgen Partner in Type 2 Diabetes 2Array BioPharma and Amgen Partner in Type 2 Diabetes 3Array BioPharma and Amgen Partner in Type 2 Diabetes 4Array BioPharma and Amgen Partner in Type 2 Diabetes 5Array BioPharma and Amgen Partner in Type 2 Diabetes 6Array BioPharma and Amgen Partner in Type 2 Diabetes 7Dyadic International Extends Collaboration With The Scripps Research Institute 2Dyadic International Extends Collaboration With The Scripps Research Institute 3Dyadic International Extends Collaboration With The Scripps Research Institute 4Dyadic International Extends Collaboration With The Scripps Research Institute 5Infusion Nurses Society References Totect(R) (Dexrazoxane For Injection) as the Only Antidote for the Treatment of Anthracycline Extravasation 2Infusion Nurses Society References Totect(R) (Dexrazoxane For Injection) as the Only Antidote for the Treatment of Anthracycline Extravasation 3Infusion Nurses Society References Totect(R) (Dexrazoxane For Injection) as the Only Antidote for the Treatment of Anthracycline Extravasation 4Infusion Nurses Society References Totect(R) (Dexrazoxane For Injection) as the Only Antidote for the Treatment of Anthracycline Extravasation 5
(Date:9/15/2014)... hearing and cognitive function may play roles in one,s ... rhythm, pitch, timbre, tone durations, and formal structure in ... article explain that extremes in musical aptitude (extreme capacity/no ... of individuals having moderate aptitude. , "This is a ... underlying genes, and it is influenced to varying degrees ...
(Date:9/15/2014)... the subsequent rapid decrease in the water level of ... square kilometres of new, fertile land in eastern Finland. ... researchers has studied the role that the decrease in ... and humans. After dramatic shifts in the waterways, human ... rise to a new, innovative culture. This stemmed from ...
(Date:9/15/2014)... Sharks, swamps and a tropical rainforest teeming with life ... Yorkshire, England. But for the first time evidence of Doncaster,s ... discovered in a derelict mining tip. , Some of the ... and as well as the egg case, several horseshoe crabs ... All had been preserved in rocks that formed within ...
Breaking Biology News(10 mins):The creation of the Vuoksi River preceded a significant cultural shift 2'Jaws' lived in Doncaster 2
... its wood and cottony fibers, the kapok tree now ... for decades: the notion that African and South American ... million years ago. , Research by University of Michigan ... perhaps other rainforest--trees colonized Africa after the continents split ...
... modification to successfully manipulate the growth in height of trees, ... similar to normal trees – but after several years of ... few inches. , This is a “proof of concept” that ... It opens the door to a wide variety of new ...
... that will be published online in advance of its July ... colleagues (The Salk Institute and HHMI) demonstrate that endosomes can ... animals. , "These findings will influence our thinking of the ... why cells use them as signaling compartments," noted lead author ...
Cached Biology News:The kapok connection -- Study explains rainforest similarities 2Researchers demonstrate way to control tree height 2Researchers demonstrate way to control tree height 3