Navigation Links
Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery

imally-treated complex biological matrix, was compared at unit and enhanced mass-resolution. In this manner, an assessment of the improvement in LOQ for this compound at enhanced mass-resolution can be made.

Experimental

Chemicals and Reagents
Rat plasma was purchased from Bioreclamation Inc. (Hicksville,NY, USA). Optima-grade (99.9%) acetonitrile and methanol were obtained from Fisher Scientific Co. (Pittsburgh, PA,USA), ammonium acetate from Sigma Chemical Co.(St.Louis, MO,USA), and glacial acidic acid (99.99+%) from Aldrich Chemical Company (Milwaukee, WI,USA). Water was purified using a compact ultra-pure water system (EASYpure UV, Dubuque, IA,USA).

Sample Preparation
150 μL of internal standard (IS) in acetonitrile solution (conc. 10 ng/mL) was added to 50 μL of drug discovery compound A in rat plasma (concs. 250 ng/mL). After plasma precipitation, the mixture was vortexed for 30 s, and then centrifuged for 10 min. The supernatant was transferred to a clean 96-well plate for LC/MS/MS analysis.

HPLC
HPLC Column: Symmetry C18, 50 4.6 mm, 5 μm (Waters Corporation, Milford, MA,USA)
Mobile Phase A: 0.01 M ammonium acetate in MeOH/H2O (20:80, v/v)
Mobile Phase B: 0.01 M ammonium acetate in MeOH + 0.6 mL/L 10% acetic acid
Flow Rate: 0.8 mL/min Gradient: 5% B for 0.5 min, 5% B to 95%B for 0.5 min, 2.0 min hold at 95% B, return to 5% B at 3.0 min, 3.0 min run time
Divert to MS at 0.8 min Divert to waste at 2.0 min Injection Vol.: 8 μL
Column Temp.: Ambient Temperature
Autosampler Temp: 5C
Retention Time: Compound A - 1.2 min
Internal Standard - 1.5 min

Mass Spectrometer
 Mass Spectrometer: Finnigan TSQ Quantum Discovery (ThermoElectron,San Jose,CA,US
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Increased Sensitivity in Microarray Analysis
2. Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell
3. Improved Sensitivity for Staining RNA with SYBR Gold Stain
4. Enhancing Ruggedness and Full-Scan MS Sensitivity Using Ion Sweep Technology
5. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
6. Femtogram Sensitivity of the Antipsychotic Drugs Raclopride and Ritanserin Using a Varian 1200L LC/MS/MS
7. Maintaining High Sensitivity when Working with Small-Volume Samples
8. High Sensitivity, Wide Dynamic Range, Horseradish Peroxidase (HRP) ELISA Using the LMax Microplate Luminometer (MaxLine Application Note #41)
9. Sensitivity and Quantitation using a Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer
10. Trace Analysis Optimizing Sensitivity Finnigan Surveyor PDA Detector
11. A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity
Post Your Comments:
(Date:5/22/2013)... May 22, 2013 Industry leaders ... VA for the Oak Ridge National Laboratory’s ... take place at the Hilton Alexandria Mark Center. ... Technologies 2013 conference on June 18 - ... , AdSave, American Elements, Applied Research Associates, ARA, ...
(Date:5/22/2013)... BUFFALO, N.Y. , May 22, 2013 /PRNewswire/ ... exclusive license for a patent pending novel genomic ... a molecular diagnostic test that could help in ... (NEPC) prostate cancer.  Neuroendocrine prostate ... adenocarcinoma (PCA) that can arise de novo, but ...
(Date:5/22/2013)... AudioNotch is the ... treatment. AudioNotch is pleased to announce a partnership ... in both British Columbia and Alberta, Destination Hearing ... services. AudioNotch's tinnitus treatment program is now available ... Destination Hearing clinic, patients have the detection of ...
(Date:5/22/2013)... 22, 2013 A recent study from ... athletes to find that those who took probiotics were able ... from the study showed that in extreme heat conditions, probiotics ... their adult acne treatment explains what this new ... may help their skin condition. , While researchers tote ...
Breaking Biology Technology:Biodetection Technologies 2013 and Oak Ridge National Lab’s Biosurveillance Symposium to Bring Together Leading Organizations in Alexandria, VA from June 17-19, 2013 2Biodetection Technologies 2013 and Oak Ridge National Lab’s Biosurveillance Symposium to Bring Together Leading Organizations in Alexandria, VA from June 17-19, 2013 3Biodetection Technologies 2013 and Oak Ridge National Lab’s Biosurveillance Symposium to Bring Together Leading Organizations in Alexandria, VA from June 17-19, 2013 4Biodetection Technologies 2013 and Oak Ridge National Lab’s Biosurveillance Symposium to Bring Together Leading Organizations in Alexandria, VA from June 17-19, 2013 5Empire Genomics Licenses Novel DNA Biomarker for Use in Diagnosing and Creating a Companion Diagnostic Test for Neuroendocrine Prostate Cancer 2AudioNotch Tinnitus Treatment Launches Partnership With Destination Hearing 2Adult Acne Treatment, Probiotic Action Shares Insight on New Findings Between Probiotics and Heat Tolerance, and What It Means for Acne Sufferers 2
... WASHINGTON, June 15 Congressional Quarterly presents a 360-degree ... of follow-on versions of biotech drugs. This CQ Forum ... interest groups, and leading researchers regarding the complex structures ... possible? Will the availability of these products save money ...
... ADVENTRX Pharmaceuticals, Inc. (NYSE Amex: ANX ) ... of shares of convertible preferred stock pursuant to a registered ... of approximately $2 million. The preferred stock is convertible into ... investor at a price of $0.1105 per share. , ...
... Zentaris Inc. (NASDAQ: AEZS ; TSX: AEZ), ... oncology, today reported that patient follow-up in the open-label ... in benign prostatic hyperplasia (BPH) with its lead endocrinology ... the end of this week. Therefore, data analysis and ...
Cached Biology Technology:ADVENTRX Pharmaceuticals Announces Closing of Financing 2ADVENTRX Pharmaceuticals Announces Closing of Financing 3AEterna Zentaris to Report Data from Safety Study of Phase 3 Program in Benign Prostatic Hyperplasia with Cetrorelix Ahead of Schedule 2AEterna Zentaris to Report Data from Safety Study of Phase 3 Program in Benign Prostatic Hyperplasia with Cetrorelix Ahead of Schedule 3AEterna Zentaris to Report Data from Safety Study of Phase 3 Program in Benign Prostatic Hyperplasia with Cetrorelix Ahead of Schedule 4AEterna Zentaris to Report Data from Safety Study of Phase 3 Program in Benign Prostatic Hyperplasia with Cetrorelix Ahead of Schedule 5
(Date:5/22/2013)... Calif., May 22, 2013 Early screening for prostate ... pregnancy testing is for women, thanks to UC Irvine ... American Chemical Society . , After more than a ... way to clearly identify clinically usable markers for prostate ... detected far sooner, with greater accuracy and at dramatically ...
(Date:5/21/2013)... monitoring" device attached to a whale entangled in fishing ... lines changed a whale,s diving and swimming behavior. The ... eat and migrate, depletes their energy as they drag ... a slow death. , The scientists in this entanglement ... a two-year-old female North Atlantic right whale called Eg ...
(Date:5/21/2013)... that a class of pharmaceuticals can both prevent and ... known as "TSPO ligands," are currently used for certain ... of TSPO ligand in young adult mice when pathology ... when pathology was quite severe," said lead researcher Christian ... ligand reduced measures of pathology and improved behavior at ...
Breaking Biology News(10 mins):UCI chemists devise inexpensive, accurate way to detect prostate cancer 2UCI chemists devise inexpensive, accurate way to detect prostate cancer 3Study reveals how fishing gear can cause slow death of whales 2Study reveals how fishing gear can cause slow death of whales 3Drugs found to both prevent and treat Alzheimer's disease in mice 2
... Researchers at the Umeå Plant Science Centre at the Swedish ... in our understanding of how plants control their flowering. In ... they show how a small molecule that is formed in ... it induces the formation of flowers. This knowledge can lead ...
... you burned your finger on a hot stove? Imagine what ... or feet all the time and know there's virtually nothing ... neuropathic pain, and it's a common complication of many diseases ... this type of pain, which is caused by damage to ...
... the University of Chicago and Argonne National Laboratory ... to study the relationship between biochemical fluctuations within ... it interacts with other cells and its environment. ... in cancer research, drug development and combating bioterrorism. ...
Cached Biology News:Long-sought flower-inducing molecule found 2Scientists use gene transfer technology and common virus to block neuropathic pain in animals 2Scientists use gene transfer technology and common virus to block neuropathic pain in animals 3Scientists use gene transfer technology and common virus to block neuropathic pain in animals 4Scientists use gene transfer technology and common virus to block neuropathic pain in animals 5Scientists create digital bacteria to forge advances in biomedical research 2Scientists create digital bacteria to forge advances in biomedical research 3
Candida (IgG) -Ab EIA Sample Size: 5 l...
Automated centrifuge for the processing of microplates and tubes; can be integrated into automated process using inexpensive robotics due to its unique sample handling platform....
OptiBuffer is a fully optimised medium, designed for,the electroporation of eukaryotic cells. OptiBuffer,improves both transfection efficiencies and survival,rates over PBS or other standard culture...
...
Biology Products: