Additionally, Transcriptor is a fast reverse transcriptase, requiring only 30 minutes for first-strand cDNA synthesis, which results in a reduction of the total reaction time.

Transcriptor Reverse Transcriptase is the product of choice to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro transcribed RNA) from a variety of sources, using conventional thermal cyclers or real-time PCR instruments (e.g., the LightCycler Instrument) for the following applications:

• Synthesis of first-strand cDNA for use in subsequent amplification reactions

• RT-PCR of GC-rich RNA templates

• Incorporation of Cyanine 3-, Cyanine 5-, DIG-, Biotin-, and aminoallyl-modified nucleotides during cDNA synthesis

• Retrieval and cloning of the 5′ and 3′ termini of mRNA by RACE (Rapid Amplification of cDNA Ends)

• Generation of cDNA libraries with large inserts.

Transcriptor Reverse Transcriptase is supplied in storage buffer with a volume activity of 20 U/l. A 5x concentrated reaction buffer is also provided.

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