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Increase the Power and Sensitivity for Your cDNA,,,Synthesis with the New Transcriptor Reverse Transcriptase

and amplified in a subsequent PCR.

Rely on a Multi-Purpose Enzyme Tested in a Wide Variety of Assays

Transcriptor Reverse Transcriptase shows premium performance with conventional thermal cyclers and the LightCycler Instrument. Compared to other reverse transcriptases, it is produced under strict regulatory conditions and function-tested in both systems ensuring functionality in end-point and real-time PCR. Additional specifications, such as bioburden, increase the safety level when working with RNA.

Efficiently Label RNA

Efficient incorporation of modified nucleotides during cDNA synthesis is possible, making Transcriptor Reverse Transcriptase an important labeling enzyme in, for example, microarray applications. Labeling with Cyanine 3-, Cyanine 5-, aminoallyl-, DIG-, and Biotin-dUTP was tested; the result was high-efficiency incorporation of these modified nucleotides.

Shorten the Reaction Time

With Transcriptor Reverse Transcriptase there is no need to perform an extra RNase H incubation step following cDNA synthesis as recommended to remove RNA complementary to cDNA when using a RNase H-- mutated reverse transcriptase. RNase H--mutated reverse transcriptase can limit the sensitivity of RT-PCR detection as shown for the gene NCX2, which was detected much more readily when a RNase H step was included after the reverse transcription [1]. If the RNA template is not degraded after first-strand cDNA synthesis, it can bind to the newly synthesized cDNA and restrict its accessibility to primers during subsequent PCR amplification. RNase H-mediated destruction of the template can prevent this problem and improve the sensitivity of RT-PCR analysis.


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