Navigation Links
Increase the Power and Sensitivity for Your cDNA,,,Synthesis with the New Transcriptor Reverse Transcriptase

and amplified in a subsequent PCR.

Rely on a Multi-Purpose Enzyme Tested in a Wide Variety of Assays

Transcriptor Reverse Transcriptase shows premium performance with conventional thermal cyclers and the LightCycler Instrument. Compared to other reverse transcriptases, it is produced under strict regulatory conditions and function-tested in both systems ensuring functionality in end-point and real-time PCR. Additional specifications, such as bioburden, increase the safety level when working with RNA.

Efficiently Label RNA

Efficient incorporation of modified nucleotides during cDNA synthesis is possible, making Transcriptor Reverse Transcriptase an important labeling enzyme in, for example, microarray applications. Labeling with Cyanine 3-, Cyanine 5-, aminoallyl-, DIG-, and Biotin-dUTP was tested; the result was high-efficiency incorporation of these modified nucleotides.

Shorten the Reaction Time

With Transcriptor Reverse Transcriptase there is no need to perform an extra RNase H incubation step following cDNA synthesis as recommended to remove RNA complementary to cDNA when using a RNase H-- mutated reverse transcriptase. RNase H--mutated reverse transcriptase can limit the sensitivity of RT-PCR detection as shown for the gene NCX2, which was detected much more readily when a RNase H step was included after the reverse transcription [1]. If the RNA template is not degraded after first-strand cDNA synthesis, it can bind to the newly synthesized cDNA and restrict its accessibility to primers during subsequent PCR amplification. RNase H-mediated destruction of the template can prevent this problem and improve the sensitivity of RT-PCR analysis.


'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Increased Sensitivity in Microarray Analysis
2. Increase Protein Activity and Yield
3. Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell
4. Increase Your Organizations Productivity with LabVIEW
5. Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery
6. 140-Fold Increase of Protein Achieved in Pichia Fermentation
7. A Versatile Power Supply for All Electrophoresis Applications
8. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
9. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
10. Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations
11. National Instruments DIAdem -- Complete Your Technical Data Management Framework with Powerful Off-line Analysis, Visualization, and Report Generation
Post Your Comments:
(Date:7/10/2014)... (PRWEB) July 10, 2014 OMICS Group’s ... techniques during 18-20 August, 2014 at Double Tree by ... the recent developments in Analytical & Bioanalytical research methods ... , Speaking on this occasion, Dr. Srinubabu Gedela, MD ... Acta conference is a remarkable one in bringing a ...
(Date:7/10/2014)... Capital, a firm specializing in acquiring and managing food ... the Slim-Fast brand from Unilever. Unilever will retain a ... were not disclosed. Slim-Fast is a branded ... shakes, powders, bars and snacks to retail customers throughout ... United Kingdom and Ireland.  The ...
(Date:7/10/2014)... the fight against terrorism with the creation of a ... the use of light and special glass fibres. , ... Chemical , the researchers describe a novel optical fibre ... as 6.3 ppm (parts per million). It requires an ... explosives detection has involved looking for metals that encase ...
(Date:7/10/2014)... RICHLAND, Wash. The lithium-ion batteries that power our ... run longer on a single charge with the help ... the porous material to replace the graphite traditionally used ... more than 10 times the energy storage capacity of ... lithium-ion battery electrode was published today in Nature ...
Breaking Biology Technology:A Critical Review of Analytical Techniques for Improved Living Standards 2A Critical Review of Analytical Techniques for Improved Living Standards 3A Critical Review of Analytical Techniques for Improved Living Standards 4Kainos Capital Acquires Slim-Fast From Unilever 2Detecting trace amounts of explosives with light 2Silicon sponge improves lithium-ion battery performance 2Silicon sponge improves lithium-ion battery performance 3
... 9 Genstruct Inc., a,biotechnology company translating the complexity ... that Andrea Matthews,(age 25), manager of its Scientific Programs, ... Watch Under 30." "We are proud to have ... team," said Dr. Keith O. Elliston, president and CEO ...
... SciQuest will Save Time and Money, ... 9 The University,HealthSystem Consortium (UHC), an ... partners, and SciQuest, a provider of e-procurement,technology ... announced a partnership to provide organizations participating ...
... NATICK, Mass., Oct. 9 Boston Scientific,Corporation (NYSE: ... of its,CONFIENT(TM) implantable cardioverter defibrillator (ICD). This approval,represents ... treat sudden cardiac death (SCD). The CONFIENT ... is,designed to reduce unnecessary right ventricular pacing. This ...
Cached Biology Technology:Genstruct's Andrea Matthews Selected for Mass High Tech's 'People to Watch Under 30' 2University HealthSystem Consortium Chooses SciQuest to Optimize Medical Research Procurement 2University HealthSystem Consortium Chooses SciQuest to Optimize Medical Research Procurement 3Boston Scientific Announces European Approval of Next-Generation Implantable Defibrillator 2Boston Scientific Announces European Approval of Next-Generation Implantable Defibrillator 3
(Date:7/11/2014)... exists that cannabinoid receptor type 1 can ... influx, and reduce neurotransmitter release. However, some ... can increase extracellular Ca2+ influx and increase ... team, Tongji Hospital Affiliated to Tongji Medical ... China used whole cell voltage-clamp and calcium ...
(Date:7/11/2014)... brains of all vertebrates, information is transmitted through ... chemical signal to be passed from one brain ... most abundant type of synapse, can be either ... learning, memory, perception and cognition, and the balance ... function. For instance, every time we learn ...
(Date:7/10/2014)... June 27, 2014  Pomerantz LLP has filed ... ("Provectus" or the "Company")(NYSE-MKT: PVCT) and certain of ... States District Court, Middle District of ... of a class consisting of all persons or ... between December 17, 2013 and May 22, 2014, ...
Breaking Biology News(10 mins):Blame it on the astrocytes 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 3
... it,s known that animals flourish in regions where the ... study set out to discover whether that same relationship ... Jurassic when dinosaurs roamed the Earth. "The assumption ... modern ecosystems," said paleontologist and lead author Timothy S. ...
... adolescents lose their protection from hepatitis B virus (HBV) ... infants. Results in the January 2013 issue of ... of the American Association for the Study of Liver ... HBeAg) and teens whose immune system fails to remember ...
... "Woodstock" song by Joni Mitchell, it turns out, was onto ... Chicago evolutionary biologist, Neil Shubin, PhD, makes that connection between ... "The Universe Within: Discovering the Common History of Rocks, Planets, ... Inner Fish." Where "Your Inner Fish" goes back ...
Cached Biology News:Study finds Jurassic ecosystems were similar to modern: Animals flourish among lush plants 2Study finds Jurassic ecosystems were similar to modern: Animals flourish among lush plants 3Study finds Jurassic ecosystems were similar to modern: Animals flourish among lush plants 4Teens susceptible to hepatitis B infection despite vaccination as infants 2New book connects the human community to its cosmic roots 2New book connects the human community to its cosmic roots 3
... receptors constitute the principal excitatory ... classes of glutamate receptors exist: ... Ionotropic glutamate receptors are oligomeric ... NMDA1-3, KAI-2) which comprise ligand-gated ...
... Block Moto Alpha unit with motorized heated lid ... that is useful for microarrays and chips. The ... blocks. The sealing force of the motorized lid ... to give repeatable results. This interchangeable Moto Alpha ...
Caspr2 Antigen: Fusion protein amino acids 96-1265 (extracellular domain) of human Caspr2 Accession Number: NP_054860...
... N,N- bis- (3-D-Gluconamidopropyl)deoxycholamide ... of CHAPS and CHAPSO. Has reduced electrostatic ... exchange chromatography on DEAE-cellulose. Aggregation number: 8-16. ... in H 2 O. Aggregation number 8 ...
Biology Products: