( As little as 100 pg of tota...)Increase the Power and Sensitivity for Your cDNA,,,Synthesis with the New Transcriptor Reverse Transcriptase

As little as 100 pg of total RNA is detected, in combination with the new FastStart High Fidelity PCR System. This enables the analysis of low expressed RNA and RNA when sample material is limited (Figure 3).

• In one-step RT-PCR using conventional thermal cyclers,

As little as 1 pg of total RNA or 100 fg mRNA is detected, in combination with the Expand High Fidelity PCR System. This permits the analysis of low expressed RNA in one-step RT-PCR.

Obtain Full-Length cDNA Synthesis

cDNA up to 14 kb in length can be generated with Transcriptor Reverse Transcriptase, making it the product of choice for reverse transcription of very long eukaryotic mRNA, as shown in Figure 4 for human dystrophin, one of the longest mRNAs with a length of 14 kb. To achieve high sensitivity and yield in the following PCR, use FastStart Taq DNA Polymerase or the new FastStart High Fidelity Enzyme Blend. Both allow hot-start PCR and are available as single reagents or included in different LightCycler Kits (only Fast Start Taq DNA Polymerase). For amplification of longer fragments, the Expand Long Template PCR System is recommended.

Obtain the Power to Reverse Transcribe the Most Difficult RNAs

Due to the very high thermostability of Transcriptor Reverse Transcriptase (up to 65C) compared to other thermostable reverse transcriptases, robust reverse transcription of GC-rich RNA with high secondary structure can now be easily achieved (Figure 5). In combination with FastStart Taq DNA Polymerase and the GC-RICH Resolution Solution, Transcriptor Reverse Transcriptase is the product of choice when difficult RNAs must be reverse transcribed
'"/>

Source:


Page: All 1 2 3 4
Related biology technology :

1. Increased Sensitivity in Microarray Analysis
2. Increase Protein Activity and Yield
3. Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell
4. Increase Your Organizations Productivity with LabVIEW
5. Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery
6. 140-Fold Increase of Protein Achieved in Pichia Fermentation
7. A Versatile Power Supply for All Electrophoresis Applications
8. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
9. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
10. Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations
11. National Instruments DIAdem -- Complete Your Technical Data Management Framework with Powerful Off-line Analysis, Visualization, and Report Generation