As little as 100 pg of total RNA is detected, in combination with the new FastStart High Fidelity PCR System. This enables the analysis of low expressed RNA and RNA when sample material is limited (Figure 3).

• In one-step RT-PCR using conventional thermal cyclers,

As little as 1 pg of total RNA or 100 fg mRNA is detected, in combination with the Expand High Fidelity PCR System. This permits the analysis of low expressed RNA in one-step RT-PCR.

Obtain Full-Length cDNA Synthesis

cDNA up to 14 kb in length can be generated with Transcriptor Reverse Transcriptase, making it the product of choice for reverse transcription of very long eukaryotic mRNA, as shown in Figure 4 for human dystrophin, one of the longest mRNAs with a length of 14 kb. To achieve high sensitivity and yield in the following PCR, use FastStart Taq DNA Polymerase or the new FastStart High Fidelity Enzyme Blend. Both allow hot-start PCR and are available as single reagents or included in different LightCycler Kits (only Fast Start Taq DNA Polymerase). For amplification of longer fragments, the Expand Long Template PCR System is recommended.

Obtain the Power to Reverse Transcribe the Most Difficult RNAs

Due to the very high thermostability of Transcriptor Reverse Transcriptase (up to 65C) compared to other thermostable reverse transcriptases, robust reverse transcription of GC-rich RNA with high secondary structure can now be easily achieved (Figure 5). In combination with FastStart Taq DNA Polymerase and the GC-RICH Resolution Solution, Transcriptor Reverse Transcriptase is the product of choice when difficult RNAs must be reverse transcribed
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