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Increase the Power and Sensitivity for Your cDNA,,,Synthesis with the New Transcriptor Reverse Transcriptase

Transcriptor Reverse Transcriptase is a new recombinant reverse transcriptase (RT) expressed in E. coli. The enzyme has RNA-directed DNA polymerase activity, DNA-dependent DNA polymerase activity, unwinding activity, and, importantly, RNase H activity.

Due to the special recombinant structure of Transcriptor Reverse Transcriptase, the individual enzymatic activities are resulting in a number of unique and beneficial properties.

Achieve High Sensitivity
  • In two-step RT-PCR with the LightCycler Instrument,
Transcriptor Reverse Transcriptase has a broad dynamic detection range of 12 1.2 x 109 copies of in vitro transcribed RNA (Figure 1). This enables analysis of genes with very low or extremely high expression levels.

A linear quantification over 108-fold range of input RNA can be performed with the same efficiency with Transcriptor Reverse Transcriptase, making expression analysis of induced or repressed genes possible (Figure 1).

The high sensitivity of Transcriptor Reverse Transcriptase compared to other reverse transcriptases improves the performance of existing assays and allows performance of assays that were not previously possible (Figure 2).

High fluorescence intensities and well-shaped curves with identical distances for cDNA dilutions lead to clear and interpretable results compared to competitor products (Figure 2).

  • In two-step RT-PCR using conventional thermal cyclers,

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