( ...)Increase the Power and Sensitivity for Your cDNA,,,Synthesis with the New Transcriptor Reverse Transcriptase

Transcriptor Reverse Transcriptase is a new recombinant reverse transcriptase (RT) expressed in E. coli. The enzyme has RNA-directed DNA polymerase activity, DNA-dependent DNA polymerase activity, unwinding activity, and, importantly, RNase H activity.

Due to the special recombinant structure of Transcriptor Reverse Transcriptase, the individual enzymatic activities are resulting in a number of unique and beneficial properties.

Achieve High Sensitivity

• In two-step RT-PCR with the LightCycler Instrument,
  

Transcriptor Reverse Transcriptase has a broad dynamic detection range of 12 1.2 x 109 copies of in vitro transcribed RNA (Figure 1). This enables analysis of genes with very low or extremely high expression levels.

A linear quantification over 108-fold range of input RNA can be performed with the same efficiency with Transcriptor Reverse Transcriptase, making expression analysis of induced or repressed genes possible (Figure 1).

The high sensitivity of Transcriptor Reverse Transcriptase compared to other reverse transcriptases improves the performance of existing assays and allows performance of assays that were not previously possible (Figure 2).

High fluorescence intensities and well-shaped curves with identical distances for cDNA dilutions lead to clear and interpretable results compared to competitor products (Figure 2).

• In two-step RT-PCR using conventional thermal cyclers,
  

'"/>

Source:


Page: All 1 2 3 4
Related biology technology :

1. Increased Sensitivity in Microarray Analysis
2. Increase Protein Activity and Yield
3. Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell
4. Increase Your Organizations Productivity with LabVIEW
5. Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery
6. 140-Fold Increase of Protein Achieved in Pichia Fermentation
7. A Versatile Power Supply for All Electrophoresis Applications
8. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
9. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
10. Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations
11. National Instruments DIAdem -- Complete Your Technical Data Management Framework with Powerful Off-line Analysis, Visualization, and Report Generation