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Increase Protein Activity and Yield

ActivePro In Vitro Translation Kit


Higher yields of protein than reticulocyte lysate systems
Fully compatible with eukaryotic protein translation
Higher biological activity than other E. coli lysate systems
Easy to use

For researchers studying protein function and protein-protein interactions, Ambion's ActivePro In Vitro Translation Kit is the ideal tool. ActivePro uses an E. coli lysate to synthesize high yields of functional protein for studies where properly folded active protein is a necessity. Furthermore, research data show that ActivePro is capable of efficiently synthesizing multiple proteins in a single reaction--an important feature for protein interaction studies.

Higher Yields than Retic Systems
Translation of a sequence in ActivePro typically yields greater than 1000-fold more protein than coupled reticulocyte lysate systems. What's more, ActivePro synthesizes protein at a very high rate (several hundred micrograms per hour per ml of reaction), with the reaction 80% complete in just 1 hour. In contrast, typical coupled retic kits require 1.5 hours longer incubation. An efficient energy regeneration system and a large supply of amino acids included in the ActivePro Kit support faster protein synthesis and higher yields.

Coexpression of Multiple Proteins
Comparison data with competing kits have already illustrated ActivePro's superior ab
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