Navigation Links
Increase Protein Activity and Yield

ActivePro In Vitro Translation Kit


Higher yields of protein than reticulocyte lysate systems
Fully compatible with eukaryotic protein translation
Higher biological activity than other E. coli lysate systems
Easy to use

For researchers studying protein function and protein-protein interactions, Ambion's ActivePro In Vitro Translation Kit is the ideal tool. ActivePro uses an E. coli lysate to synthesize high yields of functional protein for studies where properly folded active protein is a necessity. Furthermore, research data show that ActivePro is capable of efficiently synthesizing multiple proteins in a single reaction--an important feature for protein interaction studies.

Higher Yields than Retic Systems
Translation of a sequence in ActivePro typically yields greater than 1000-fold more protein than coupled reticulocyte lysate systems. What's more, ActivePro synthesizes protein at a very high rate (several hundred micrograms per hour per ml of reaction), with the reaction 80% complete in just 1 hour. In contrast, typical coupled retic kits require 1.5 hours longer incubation. An efficient energy regeneration system and a large supply of amino acids included in the ActivePro Kit support faster protein synthesis and higher yields.

Coexpression of Multiple Proteins
Comparison data with competing kits have already illustrated ActivePro's superior ab ility to synthesize protein with higher biological activity from both prokaryotic and eukaryotic transcripts. Now, more recent data shows ActivePro's ability to synthesize higher yields of multiple proteins in the same reaction. Two viral proteins, protein A and protein B, were synthesized in separate reactions using ActivePro and two other commercial E. coli lysate systems. Figure 1 illustrates the 2-3 fold greater yield of both proteins with ActivePro compared with the competition.

Figure 1. Expression Yields from Two Viral Proteins. DNA templates for protein A and protein B were transcribed/translated in vitro using Ambion's ActivePro Kit (1 hour reaction at 37C) and 2 other commercially available E. coli lysate kits, according to the recommended protocol. Each reaction included 20 Ci 35S-labeled methionine. Yield was assayed by TCA precipitation, and, post-acetone precipitation, 5 l of the reaction was analyzed by SDS-PAGE.


This same translation efficiency also holds true when coexpressing multiple proteins in the same reaction (Figure 2). Cell-free coexpression of two plasmids in the same reaction is useful in interaction studies such as ligand binding experiments, receptor studies, and protein-protein interaction studies. ActivePro has the proven ability not only to coexpress multiple proteins, but to do so with higher yields than the competition.

Figure 2. Cell-free Coexpression of Multiple Proteins. The DNA templates for protein A and protein B (cloned into the included vector) were coexpressed in the same reaction using the ActivePro Kit (1 hour reaction at 37C). The final reaction mixture was analyzed by SDS-PAGE.


Easy to Use
ActivePro is simple to use. You provide only a labeled amino acid (optional) and a DNA template that contains your gene of interest, a T7 promoter, ribosome binding site, and T7 terminator. The DNA template can be easily constructed by inserting your gene of interest into the multiple cloning site of Ambion's control plasmid included in the kit. In a single tube, simply premix the reagents included in the kit and add your DNA template, labeled amino acid, if desired, and incubate at 37C for 1 hour. The resulting biologically active protein is then ready for further functional analysis.


back to top


Ordering Information
Cat# Product Name Size 1295 ActivePro In Vitro Translation Kit 25 rxns
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
2. Increased Sensitivity in Microarray Analysis
3. Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell
4. Increase Your Organizations Productivity with LabVIEW
5. Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery
6. 140-Fold Increase of Protein Achieved in Pichia Fermentation
7. A New C-Terminal GST Vector for Protein Production in S. pombe
8. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
9. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
10. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
11. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:1/16/2017)... , ... January 16, 2017 , ... ... laws precedential publication its decision on the appeal filed by India-based Dishman Pharmaceutical ... breach of contract against DPCL and one of its Dishman Group’s 100% wholly ...
(Date:1/16/2017)... ... January 16, 2017 , ... ... prestigious Tibbetts Award from the U.S. Small Business Administration. The Tibbetts ... significant economic or social impact […] and are considered the best of the ...
(Date:1/14/2017)... CA (PRWEB) , ... January 14, 2017 , ... ... Proximo™, a new service providing complete end-to-end genome assemblies to researchers around the ... genomes eliminates a major obstacle in answering a wide range of scientific questions. ...
(Date:1/13/2017)... Research and Markets has announced the addition of the ... report to their offering. ... The biosimilars market is expected to reach USD ... a CAGR of 26.3%. The global biosimilars market ... Factors such as rising incidence of various diseases, increasing demand for ...
Breaking Biology Technology:
(Date:1/6/2017)... Colo. , Jan. 5, 2017  SomaLogic ... the "Digital Life Alliance" established by iCarbonX, the ... 2015 to build a "Global Digital Health Ecosystem ... on a combination of individual,s biological, behavioral and ... the agreement between the companies, SomaLogic will provide ...
(Date:12/22/2016)... , Dec. 20, 2016  As part of its longstanding ... leading personal genetics company, recently released its latest children,s book, ... The book focuses on the topics of inheritance and variation ... Standards (NGSS) taught in elementary school classrooms in the US. ... series by illustrator Ariana Killoran , whose previous book ...
(Date:12/16/2016)... 2016   IdentyTechSolutions America LLC , a ... solutions and a cutting-edge manufacturer of software and ... offering seamless, integrated solutions that comprise IDT biometric ... solutions provide IdentyTech,s customers with combined physical identification ... crime and theft. "We are proud ...
Breaking Biology News(10 mins):