Navigation Links
Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light


Introduction
Amplification of DNA through the polymerase chain reaction (PCR) is a highly sensitive method capable of copying millions of DNA molecules from just a single molecule.1 Because of the extreme sensitivity of this method, any carry-over product from previous reactions can result in false positives or extra bands. Carry-over contamination can be avoided by using positive displacement pipettes, sterile and autoclaved reagents, and dedicated areas for template preparation away from template amplification.

In addition, previous reports have noted that UV light is also an effective means of preventing carry-over contamination.2,3,4 High intensity UV light induces the formation of thymine dimers which block the progress of the Taq polymerase, and thus contaminating carry-over products are not amplified. In this bulletin, we describe a modified sterilization method using the GS Gene Linker UV chamber to destroy contaminating amplicon or carry-over molecules in microcentrifuge tubes prior to amplification.


Materials and Methods
Amplification of DNA was performed using genomic DNA from lambda phage and purified amplified human β-actin DNA. All of the tubes used in the amplification of a 500 bp fragment of lambda phage contained 50 ng of total lambda genomic DNA suspended in 5 l of distilled water. All of the tubes for the amplification of the amplicon DNA contained 20 ng of amplified β-actin product suspended in 2 l of distilled water. For the experimental UV tests, tubes were placed in the middle of the GS Gene Linker oven and were either capped and placed on their side or kept open and held in
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
2. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
3. InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C
4. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
5. Oil-Free PCR Using the Hot Top Assembly
6. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
7. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
8. Using XL1-Red Mutator Strain to Generate Esterase Variants
9. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
10. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
11. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
(Date:12/15/2014)... Israel , Dec. 15, 2014 /PRNewswire/ ... a leading developer of adult stem cell technologies for ... Therapeutics Ltd. was awarded a grant of approximately $1.1 ... the Chief Scientist (OCS).  This is the eighth year ... from the Office of the Chief Scientist, which is ...
(Date:12/15/2014)... NEW YORK , Dec. 15, 2014  GlassesOff ... Yuval Bar-Gil as an independent director of ... was founder of AeroScout and served as its CEO ... Decker in 2012, a $240 million transaction. Recognized as ... under Mr. Bar-Gil,s leadership, AeroScout provided Wi-Fi -based RFID ...
(Date:12/15/2014)... COPENHAGEN, Denmark , Dec. 15, 2014 /PRNewswire/ ... its innovative TransCon technology to address significant unmet ... from its ongoing Phase 2 pediatric study to ... growth hormone deficiency, or GHD.  This interim analysis ... the anticipated total enrollment in the study, completing ...
(Date:12/13/2014)... , Dec. 12, 2014 /CNW/ - MaRS and Virgin Unite ... that they have joined forces to support Canadian entrepreneurs tackling ... Virgin Group Founder was at the MaRS Centre to announce ... national impact venture fund. This fund has $1 million in ... Vancouver -based Mindset Social Innovation Foundation, founded by ...
Breaking Biology Technology:BrainStorm Receives $1,100,000 for its 8th Annual Non-Dilutive Grant from Israel's Chief Scientist Office 2BrainStorm Receives $1,100,000 for its 8th Annual Non-Dilutive Grant from Israel's Chief Scientist Office 3AeroScout Founder Joins GlassesOff's Board of Directors 2AeroScout Founder Joins GlassesOff's Board of Directors 3Ascendis Pharma A/S Announces Positive Six-Month Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 2Ascendis Pharma A/S Announces Positive Six-Month Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 3Ascendis Pharma A/S Announces Positive Six-Month Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 4MaRS and Virgin Unite partner to invest in business as a force for good 2MaRS and Virgin Unite partner to invest in business as a force for good 3
... Barr Pharmaceuticals,Inc. (NYSE: BRL ) today announced ... Jersey has ruled in favor of its subsidiary, Barr,Laboratories, ... Bayer Schering,Pharma, AG in connection with Bayer Schering,s Yasmin(R) ... Peter G.,Sheridan found that the patent at issue was ...
... Iomai Corporation,(Nasdaq: IOMI ) today announced that ... before the markets open and hold a,conference call and ... March 6, 2008 at 1:30 p.m. Eastern Time., ... call on Thursday, March 6 at 1:30 p.m.,Eastern Time ...
... in a row,the capital markets succumbed to fears of inflation ... the Dow dropped over 300 points after,oil prices pushed past ... was dragged down with the Burrill Biotech Select Index,dropping 4% ... closed down,3%., "The biotech sector started 2008 on a ...
Cached Biology Technology:Barr Announces Favorable Ruling in Yasmin(R) Patent Challenge 2Barr Announces Favorable Ruling in Yasmin(R) Patent Challenge 3Barr Announces Favorable Ruling in Yasmin(R) Patent Challenge 4Iomai to Announce Fourth Quarter and Year-End 2007 Financial Results and Host Webcast on Thursday, March 6 2Biotech Drops Amid Tough Markets in February 2Biotech Drops Amid Tough Markets in February 3
(Date:11/21/2014)... VIEW, Calif. , Nov. 20, 2014 ... higher driver efficiency are piloting the North American and ... number of accidents growing, gesture recognition systems that are ... make a mark in the industry. ... the Automotive Gesture Recognition Market in Europe ...
(Date:11/18/2014)... , Nov. 17, 2014   ... and EMC collaborate to develop The Partners Data ... , The Partners Data Lake will allow researcher ... improve diagnostics, treatment and the lives of patients ... research and clinical activities across the Partners system, ...
(Date:11/12/2014)... , Nov. 12, 2014 Crossmatch™, a leading ... its U.are.U ® fingerprint readers have been deployed ... Central Mexico . The bakery chain ... eliminate payroll issues caused by employees clocking in for ... U.are.U fingerprint readers, Montparnasse relied on paper timecards and ...
Breaking Biology News(10 mins):Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 2Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 4Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 2Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 3Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 4Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 5Montparnasse Pasteleria Achieves Time and Attendance Transparency with Crossmatch U.are.U Fingerprint Readers 2
... Spring Harbor, N.Y. Much like cancer cells, plant ... normal milieu, in culture dishes, have highly unstable genomes. ... help cells cope with challenging culture conditions but inadvertently ... bits of DNA that can jump around in the ...
... change will reach a tipping point in less than ... of the planet,s coral reef ecosystems according to the ... by the Global Coral Reef Monitoring Network and the ... "Status of Coral Reefs of the World: 2008" provides ...
... State University chemist could eventually lead to a quicker ... key to treating a number of diseases, including cancer, ... and research often are made within genetically modified ... to collect into what scientists call inclusion bodies. Those ...
Cached Biology News:CSHL researchers map changing epigenetic modifications that enable transposons to run amok 2Time running out on coral reefs as climate change becomes increasing threat 2Time running out on coral reefs as climate change becomes increasing threat 3
... polyacrylamide gel electrophoresis (2D-PAGE) with biological mass ... technology platform for proteomic analysis. 2D-PAGE gels ... limited only in the dynamic range and ... Quantitative image based analysis of separated proteins ...
... supply is a highly compact, extremely reliable and ... knob and one hi-lo range switch control the ... from 20 to 250 volts. One two-position switch ... voltage to output current. For maximum safety, the ...
... a compact, lightweight, high current power supply capable ... milliamps. It is an excellent choice for PAGE, ... is also ideal for all types of electroblotting ... to automatically end a run and wil automatically ...
Rat Hepatocytes (RH) (>3,000,000 cells)...
Biology Products: