Navigation Links
In Vivo Signal Transduction Pathway Reporting Systems

on factors are not able to bind efficiently to the yeast-derived GAL4 UAS sequence. The high specificity and low background of the PathDetect systems have additional applications, including mapping a protein's position in the signal transduction pathway by introducing known inhibitors of the pathway and screening for drugs that promote or inhibit the activity of JNK, MAPK, PKA or other upstream signaling molecules (such as Ras, raf and JNKK).

Conclusions

The PathDetect in vivo signal transduction pathway reporting systems can be used to determine and map the signal transduction pathway for a gene product and to study the effects of extracellular stimuli on the JNK, MAPK or PKA signal transduction pathways. Researchers can use these systems to evaluate the in vivo role of a new gene product by the activation, inhibition or regulation of the selected signal transduction pathway. The firefly luciferase gene is used as the reporter gene in the PathDetect reporter plasmid. Therefore, the activation of these signaling pathways can be readily measured by the luciferase assay, which is convenient, sensitive and quantitative. The kits include fusion transactivator plasmids, reporter plasmids and positive and negative control plasmids that are highly purified and transfection ready.


'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
2. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
3. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
4. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
5. New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways
6. Determining the Signal-to-Noise Ratio and Optimal Photomultiplier gain setting in the GenePix 4000B
7. Versatile Reporter Vectors for Monitoring Viral Transduction
8. Improved Vectors for PathDetect Trans-Reporting Systems
9. Cis-Reporting System Monitors Intracellular Calcium Mobilization
10. Cis-Reporting System Monitors Intracellular Calcium Mobilization
11. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Vivo Signal Transduction Pathway Reporting Systems

(Date:10/22/2014)... The North American crystal oscillator market report defines and ... forecast of revenue. This market was valued at $531.4 ... million by 2018, at a CAGR of 3.3% from ... the North American crystal oscillator market report, to get ... provides a glimpse of the segmentation of the market, ...
(Date:10/22/2014)... 22, 2014 Involution Studios , ... of a new infographic, Understanding Ebola . ... beautifully designed, easy to follow informative tool for anyone ... outbreak, symptoms and prevention. , "As the news has ... 2014 Ebola outbreak represents not only a healthcare crisis ...
(Date:10/22/2014)... Diego, CA (PRWEB) October 22, 2014 ... balances from Sartorius, A & D Weighing, Rice ... Sartorius CPA Semi-Micro Balance . The Sartorius CPA ... for an affordable high-quality, precise, and user-friendly laboratory ... manufacturing of laboratory equipment, their laboratory balances are ...
(Date:10/22/2014)... 2014 Physicians Choice Laboratory Services ... menu, which enables healthcare providers to individualize therapeutic ... evidence-based results, healthcare providers are better able to ... while minimizing risks for adverse drug reactions and ... the FDA [1] more than 2.2 million adverse ...
Breaking Biology Technology:The North American crystal oscillator market is expected to reach $623.6 million by 2018 - New Report by MicroMarket Monitor 2The North American crystal oscillator market is expected to reach $623.6 million by 2018 - New Report by MicroMarket Monitor 3The North American crystal oscillator market is expected to reach $623.6 million by 2018 - New Report by MicroMarket Monitor 4Involution Studios' Ebola Infographic Provides Key Disease Information and Statistics at a Glance 2Pipette.com Launches the Addition of Sartorius CPA Semi-Micro Balance to Their Product Portfolio with Special Promotional Pricing 2Pipette.com Launches the Addition of Sartorius CPA Semi-Micro Balance to Their Product Portfolio with Special Promotional Pricing 3PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 2PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 3
(Date:10/18/2014)... stress and stress-related psychiatric disorders are associated ... the molecular mechanisms underlying this relation are ... the development of targeted preventive strategies and ... diseases. This work is presented at the ... Now an international group of researchers from ...
(Date:10/17/2014)... news release is available in German . ... millions of sperms every day in order to reproduce? And ... are the topic of the latest issue of the research ... 2014). The evolutionary biologist Steven Ramm from Bielefeld University Bielefeld ... it is not unusual for a female to copulate with ...
(Date:10/16/2014)... William and Mary,s Virginia Institute of Marine Science ... Environmental Protection Agency to identify the streams and ... develop tools to help local governments and citizens ... critically on a dataset of tidal-marsh observations first ... with this historical baseline can today,s researchers accurately ...
Breaking Biology News(10 mins):Researchers find why depression and aging linked to increased disease risk 2Sperm wars 2EPA grant will help localities conserve headwater wetlands 2EPA grant will help localities conserve headwater wetlands 3
... use of an automated, underwater cell analyzer developed at ... managers were recently able to detect a bloom of ... prevent human consumption of tainted shellfish. Shellfish beds in ... though they are expected to re-open in the next ...
... This research work is the first step to confirm ... to detect the signs of the Climatic Change through the temperatures ... of Applied Physics of the University of Granada ... maximum and minimum temperature series of every month since the end ...
... FAIRBANKS, AlaskaDuring the mid-1970s, Glenn Shaw of the Geophysical ... the notion of Arctic haze after collecting aerosol samples ... Arctic to contain relatively pure air due to its ... rocked once Shaws data indicated there was a murky ...
Cached Biology News:Underwater microscope helps prevent shellfish poisoning along Gulf Coast of Texas 2Underwater microscope helps prevent shellfish poisoning along Gulf Coast of Texas 3Scientists compiled 20th century temperature data in Spain as evidence of climatic change 2Atmospheric scientists gear up for collaborative airborne campaigns 2
Canine Serum...
NORMAL DONOR DONKEY SERUM...
Homo sapiens toll interacting protein Antigen: Recombinant Protein...
Mouse monoclonal [PRMT5-21] to PRMT5 ( Abpromise for all tested applications). entrezGeneID: 10419 SwissProtID: O14744...
Biology Products: