Navigation Links
In Vivo Signal Transduction Pathway Reporting Systems

r plasmid is shown in figure 3, panel C. The PKA protein is a known activator of the CREB protein. When the pFA-CREB plasmid was cotransfected with the pFC-PKA plasmid and the reporter plasmid, a 100-fold increase in luciferase expression was seen. Further experiments (data not shown) using a construct with a mutation in the PKA phosphorylation site of the pFA-CREB plasmid showed that the mutated fusion activator could not be activated by the PKA protein.

Activation of PathDetect Reporting Systems by Extracellular Stimuli

Figure 4

In addition to activation by uncharacterized gene products, the PathDetect trans-reporting systems can be used to study the effects of physiological stimuli and drug candidates. Figure 4 shows the activation of the pFA-Elk1 vector by 10% serum, indicating activation of the MAPK signal transduction pathway. The amount of pFA-Elk1 vector used in this experiment seems limiting because the activation obtained from 10 ng of pFA-Elk1 is 10-fold greater than the activation obtained with 1 ng. Similarly, the PathDetect c-Jun trans-reporting system can be activated by ultraviolet irradiation, and the PathDetect CREB system was activated by known PKA-activating agents such as the adenylate cyclase toxin from Bordetella pertussis and forskolin (data not shown).

Applications of the PathDetect Systems

The PathDetect c-Jun, Elk1 and CREB trans-reporting systems give specific assessment of JNK, MAPK or PKA activity. Such specificity allows the effects of uncharacterized proteins on the JNK, MAPK and PKA pathways to be determined. These reporting systems show minimal background activity because mammalian transcripti
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
2. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
3. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
4. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
5. New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways
6. Determining the Signal-to-Noise Ratio and Optimal Photomultiplier gain setting in the GenePix 4000B
7. Versatile Reporter Vectors for Monitoring Viral Transduction
8. Improved Vectors for PathDetect Trans-Reporting Systems
9. Cis-Reporting System Monitors Intracellular Calcium Mobilization
10. Cis-Reporting System Monitors Intracellular Calcium Mobilization
11. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Vivo Signal Transduction Pathway Reporting Systems

(Date:11/24/2014)... Billerica, Massachusetts (PRWEB) November 24, 2014 ... Simplify Panels ” from METTLER TOLEDO demonstrates how ... panel. , Power analytics and sample panel design ... control cycle chemistry and protect against corrosion and ... take up a significant amount of panel space. ...
(Date:11/24/2014)... , November 24, 2014 ... Workflow, honored for dynamic leadership skills, business acumen ... technical and medical information products and services, congratulates ... for Clinical Reference and Workflow, Elsevier Clinical Solutions, for ... Worth Watching ® Awards issue of Profiles ...
(Date:11/22/2014)... (PRWEB) November 21, 2014 Two new educational ... what comes next for ALS research and how the pharmaceutical ... After the Ice Bucket Challenge: Where Does the ALS Money ... Tuesday, 2 December 2014, Time: 1:30pm ET, Register ... expert speaker Dr. Merit Cudkowicz, Julianne Dorn Professor of Neurology ...
(Date:11/22/2014)... (PRWEB) November 21, 2014 Prominent ... investors will gather on December 3rd at Genetic ... Conferences series. GeneticRx will take place at the ... School and will discuss the present and future ... exon skipping, and gene editing—as well as the ...
Breaking Biology Technology:New Video Shows How to Simplify Cycle Chemistry Sample Panels to Save Time and Costs 2Elsevier Clinical Solutions' Diane Bartoli Featured In Profiles in Diversity Journal's 13th Annual Women Worth Watching Issue 2Elsevier Clinical Solutions' Diane Bartoli Featured In Profiles in Diversity Journal's 13th Annual Women Worth Watching Issue 3DrugDev Webinars in December to Explore What Comes Next for ALS Research and How We Can Make Life Easier for Clinical Sites 2Pioneering Academics and Key Industry Leaders to Discuss Rare Diseases and the Emergence of New Genetic Medicines at Genetic Rx on December 3rd 2
... IRVINE, Calif., Oct. 15 The increased emphasis ... more costly medical,product development programs and requires rapid, ... appropriately. Expertise,to effect constructive change can be found ... protect our clients from compliance errors,that can lead ...
... from the Swedish medical university Karolinska Institutet reveals completely ... that contradict previous findings. The studies, which are published ... that hair follicle stem cells can divide actively and ... "The stem cells don,t behave at all in the ...
... Vineland Research and Innovation,Centre, an independent, not-for-profit ... horticultural science and innovation in Ontario,today announced ... the new role of,Research Chair, Molecular Breeding ... is determined to become a beacon of ...
Cached Biology Technology:Ground Zero Pharmaceuticals Announces Expansion of Regulatory and Product Development Consulting Services 2Ground Zero Pharmaceuticals Announces Expansion of Regulatory and Product Development Consulting Services 3Vineland Welcomes Dr. Daryl Somers as Research Chair, Molecular Breeding and Biotechnology 2
(Date:11/18/2014)... 2014 The Parenteral Drug Association (PDA) today confirmed ... speak and at least seven more will participate in the ... Shoreham Hotel in Washington D.C. , Dec. ... significant support from the regulatory agencies in the ... in our effort to help advance the use of metrics ...
(Date:11/11/2014)...  Forensicon, Inc., a Chicago -based ... the promotion of Yaniv Schiff from Senior ... Schiff,s new role as Director, he will lead company ... examiners and provide leadership within the company,s digital forensics ... Schiff joined Forensicon in 2006 ...
(Date:11/7/2014)... , November 6, 2014 Leading Biometric ... better mobile security revolutionizing online transactions.  Companies in focus today ... Ltd. (NYSE: BABA ), Google Inc. (NASDAQ: ... eBay Inc. (NASDAQ: EBAY ) and MasterCard Inc. ... and NXTDW), a biometric authentication company focused on the ...
Breaking Biology News(10 mins):FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2Yaniv Schiff Promoted to Director of Digital Forensics at Forensicon, Inc. 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 3Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 4Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 5Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 6
... The Australian Imaging, Biomarker and Lifestyle Flagship Study of ... agreement with a major strategic alliance partner, Pfizer Australia., ... and aims to better understand the causes of Alzheimer,s ... The study is an initiative of the CSIRO ...
... CREEK, Calif. Diatoms, mighty microscopic algae, have profound influence ... breathe by capturing atmospheric carbon and in so doing, ... these photosynthetic wonders have come to acquire advantageous genes ... thrive in today,s oceans. These findings, based ...
... Ulrike Gaul, professor of molecular biology at Rockefeller ... theoretical physics at New York University and at ... first Alexander von Humboldt professorships ever to be ... for this distinction, and both applications were selected ...
Cached Biology News:Alzheimer's disease research attracts first partner 2Diatom genome helps explain success in trapping excess carbon in oceans 2Diatom genome helps explain success in trapping excess carbon in oceans 32 Alexander von Humboldt professorships go to LMU Munich 22 Alexander von Humboldt professorships go to LMU Munich 32 Alexander von Humboldt professorships go to LMU Munich 42 Alexander von Humboldt professorships go to LMU Munich 5
... in Molecular Probes. SlowFadeAntifade Kits is ... as a free radical scavenger that ... SlowFade formulation (S-2828) was designed to ... to almost zero (Figure 1); it ...
b6-2A12...
... prepared with pure chemicals according to our ... is designed to isolating intact and active ... One kit is consisted of reagents enough ... 10 20 g tissue or from ...
... EVOlyzer is Tecans new Enzyme-Linked Immunosorbent Assay ... Freedom EVOlyzer is a dedicated EVO platform ... reader, washer and incubation units operated by ... EVOlyzer is available in three sizes - ...
Biology Products: