Navigation Links
In Vivo Signal Transduction Pathway Reporting Systems

New tools to study in vivo functions of new genes and drug candidates

Li Xu * Tim Sanchez * ChaoFeng Zheng
Stratagene Cloning Systems, Inc.

Stratagene's PathDetect in vivo signal transduction pathway reporting systems are designed for specific, rapid and convenient assessment of the in vivo activation of signal transduction pathways. These reporting systems can be used to study the in vivo effects of new genes, growth factors and drug candidates on the activation of c-Jun N-terminal kinase (JNK), mitogen-activated protein kinase (MAPK), cyclic AMP-dependent protein (PKA) and other signaling molecules leading to the activation of these kinases. When a fusion activator plasmid, reporter plasmid and uncharacterized gene are cotransfected into mammalian cells, either direct or indirect phosphorylation of the fusion activator protein by the uncharacterized gene product will activate transcription of the luciferase gene from the reporter plasmid. Similarly, the effects of extracellular stimuli can be studied with these systems. Very low background and as high as 200fold activation have been observed with the PathDetect c-Jun, Elk1 and CREB transreporting systems.

Signal transduction is essential for cellular growth, differentiation and the coordination of various cellular activities in multicellular organisms. Many human diseases are caused by disturbances in signal transduction. Through standard techniques in molecular biology, hundreds of genes encoding receptors, protein kinases, phosphatases and other regulatory proteins have been cloned, and many of these gene products are implicated in signal transduction pathways. Because most of the standard cloning methods do not yield information about the function of gene products, other methods are needed in order to assess the in vivo function or activity of such proteins.

Many extracellular signals modulate cellula
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
2. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
3. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
4. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
5. New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways
6. Determining the Signal-to-Noise Ratio and Optimal Photomultiplier gain setting in the GenePix 4000B
7. Versatile Reporter Vectors for Monitoring Viral Transduction
8. Improved Vectors for PathDetect Trans-Reporting Systems
9. Cis-Reporting System Monitors Intracellular Calcium Mobilization
10. Cis-Reporting System Monitors Intracellular Calcium Mobilization
11. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Vivo Signal Transduction Pathway Reporting Systems

(Date:7/10/2014)... , July 10, 2014 Research ... addition of the "International Photonic Integrated Circuit ... Forecasts to 2019" report to their offering. ... The concept of photonic integration traces its ... century. The promise of photonic integration went unexplored ...
(Date:7/10/2014)... WASHINGTON D.C., April 15, 2014 -- According to ... danger to nuclear security comes from terrorists acquiring ... (HEU) to construct a crude nuclear explosive device. ... illicit nuclear trafficking have involved gram-level quantities, which ... methods. , According to a new study appearing ...
(Date:7/10/2014)... 17, 2014 Using microscopic polymer light resonators ... researchers at MIT,s Quantum Photonics Laboratory have developed ... the parts-per-billion range. Optical sensors are ideal for ... signal-to-noise ratio, compact, lightweight nature, and immunity to ... had been developed before, the MIT team conceived ...
(Date:7/10/2014)... basis of psychiatric disorders has been extremely challenging because ... but are insufficient to cause disease. Now investigators reporting ... journal Cell Stem Cell describe a strategy ... interact with other risk factors or environmental exposures to ... pinpoints a genetic variant that may predispose individuals to ...
Breaking Biology Technology:International Photonic Integrated Circuit (Monolithic Integration, Hybrid Integration, Module Integration) Market - Forecasts to 2019 2Sensitive detection method may help impede illicit nuclear trafficking 2Swell new sensors 2New strategy could uncover genes at the root of psychiatric illnesses 2
... ChemAxon, a software,solutions provider for cheminformatics ... their cheminformatics toolkits, Marvin & JChem. The ... Markush enumeration and,core development in JChem search, ... JChem is a suite of application programming ...
... Feb. 19 NeurogesX, Inc.,(Nasdaq: NGSX ), ... therapies, announced today that it is scheduled to ... Growth Stock Conference in,Laguna Niguel, CA on February ... will be presenting and Stephen Ghiglieri, Chief,Financial Officer, ...
... Proprietary Technology Adopted in 16% of U.S. Cardiology ... ICG Acceptance and Clinical Importance, SAN DIEGO, ... leader of BioZ(R) impedance cardiography (ICG),technology, today announced ... American College of Cardiology,s ACCEL audio series., ...
Cached Biology Technology:ChemAxon Launches Marvin and JChem Version 5: Better User Experience, Better Integration, More Flexibility and Higher Performance 2NeurogesX to Present at Roth 2008 OC Growth Stock Conference 2NeurogesX to Present at Roth 2008 OC Growth Stock Conference 3CardioDynamics BioZ(R) ICG Technology Featured in American College of Cardiology (ACC) Audio Series 2CardioDynamics BioZ(R) ICG Technology Featured in American College of Cardiology (ACC) Audio Series 3
(Date:7/10/2014)... 2014 Smart technology emphasis grows in ... improved security: NXT-ID, Inc. (OTCBB: NXTD), eBay Inc. (NASDAQ: ... Apple Inc. (NASDAQ: AAPL ), Visa Inc. (NYSE: ... NXT-ID, Inc., (OTCQB: NXTD) a biometric authentication company focused on ... second series of 30 second spots on CNBC for its ...
(Date:7/10/2014)... NXT-ID, Inc., (OTCQB: NXTD) a biometric authentication company focused on ... Pereira was interviewed on July 1 st on ... . Mr. Pereira discusses the company,s next generation smart ... how the Wocket™ aims to replace a traditional wallet with ... Harris tells Gino he has never heard of the ...
(Date:7/10/2014)... Sweden , July 7, 2014 ... its first major design win (DW). A Chinese Top 5 ... the planned start of mass production in October 2014.   ... a Chinese Top 5 smartphone OEM, which has a planned ... and a target sales volume for this phone of 3 ...
Breaking Biology News(10 mins):Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 2Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 3Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 4Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 5Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 2Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 3Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 4FPC Wins DW for 1021 Touch Sensor for Flagship Smartphone from Chinese Top 5 Smartphone OEM 2
... report discovering the receptor through which a group of life-threatening ... show that infection can be inhibited by blocking this receptor. ... on February 7, give a clue to the high lethality ... severity of infection, and point the way toward a sorely ...
... in the 1960s, the industry has continued to rapidly grow ... for farmed salmon have plummeted and, there is concern that ... "Farmed fish escaping from marine net pens might become an ... fish biologist Peter Bisson. "Net pen culture of salmon is ...
... in the January 3 issue of the Journal ... of hereditary retinoblastoma survivors developing specific subtypes of soft ... decades after the original retinoblastoma diagnosis. The findings emphasize ... the authors say. , Retinoblastoma is a rare ...
Cached Biology News:Discovery could lead to better control of hemorrhagic fever viruses 2Discovery could lead to better control of hemorrhagic fever viruses 3Farmed salmon could become an invasive species in forest streams 2Study examines risk of soft tissue sarcomas in hereditary retinoblastoma survivors 2