( identical (see Figure 2) affor...)In-Vitro Metabolism Studies Using Data-Dependent LC/MSn

identical (see Figure 2) affording an [M+H]⁺ion at m/z 510. The MS² spectra for the first six metabolites (see Figure 3) afforded ions at m/z 369, 395, 492, 352 and 169. With the exception of the ion at m/z 492 (elimination of H₂O) these ions were identical to those observed in the MS² spectra of glyburide. This indicates that the site of metabolism was the cyclohexyl ring, since the loss of the cyclohexyl moiety resulted in an identical spectrum. Additionally the MS³ spectra for these metabolites were identical to the MS³ spectrum derived from glyburide.


Scheme 1.

The MS² spectrum (see Figure 4) obtained from the metabolite at 23.87 min afforded ions at m/z 385, 367, 411, 492 and 169, indicating that the site of hydroxylation was not the cyclohexyl moiety. The data from the MS² spectrum in conjunction with the data from the MS³ spectrum (see Figure 5) allowed the fragmentation pathway to be delineated (see Scheme 2).


Figure 1. RIC m/z 510.



Figure 2. MS¹ spectra.



Figure 3. MS² spectra of early eluting metabolites.



Figure 4. MS² spectrum of metabolite at 23.87 min.



Figure 5. MS² spectrum of metabolites at 23.87 min.



Scheme 2.

The data obtained thus far were compatible with three different metabolite structures (see Figure 6). To enable a mor
'"/>

Source:


Page: All 1 2 3 4 5 6
Related biology technology :

1. Big Blue Rodent Cell Cultures for Mutagenesis Studies
2. Precursor miRNAs for Successful miRNA Functional Studies
3. DGGE Analysis for Non-Invasive Studies of Primate Diet
4. Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
5. SNPs Powerful Tools for Association Studies
6. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
7. Oil-Free PCR Using the Hot Top Assembly
8. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
9. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
10. Using XL1-Red Mutator Strain to Generate Esterase Variants
11. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System