( ...)In-Vitro Metabolism Studies Using Data-Dependent LC/MSn

P.R. Tiller, Thermo Finnigan
W.H. Schaefer, D.M. Murphy, SmithKline Beecham Pharmaceuticals

Metabolite Characterization

The data presented here can be acquired using the Thermo Finnigan LCQ Series of ion trap mass spectrometers.

Introduction

An integral part of the process by which a new drug candidate is evaluated and characterized involves the investigation of its rates and routes of metabolism. Due to their convenience, relative simplicity and reliability, in-vitro systems are used early in the drug discovery process to compare the biotransformation pathways across different species and to gain preliminary information on the metabolic routes to be expected in humans.

The current methodologies to characterize drug metabolites generally utilize LC/MS and LC/MS/MS, but frequently the data obtained is not sufficient to locate the site of metabolism on a candidate molecule. The Thermo Finnigan LCQ Series, with their ability to perform multi-stage MS fragmentation, offer MS³ and MS⁴ routinely during an HPLC run. These second and third order product ion spectra afford data that allow metabolite identification with greater specificity. An additional strength of the LCQ Series is their ability to perform automated Data-Dependent experiments. This means that the mass spectrometer makes real-time decisions about which MS experiment to perform based on the spectrum just acquired. This approach will be illustrated with the example of the analysis of metabolites derived from glyburide (glibenclamide), a potent sulfonylurea drug.^1,2

Goal

In this report, the appli
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