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In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate

to the levels of cAMP that we are dealing with. The kit also provides the option to acetylate samples to increase sensitivity further, if required.

In conclusion, the cAMP [125I] FlashPlate Assay provides a robust and efficient method for high throughput cAMP determination. In addition, the use of this technology does not stop at second messenger assessment, but can be applied to all RIA applications as well as radioligand binding and guanine nucleotide binding protein assays, development of which is eagerly awaited.

Reference

1. McHale, M., et al . 1994. FEBS Letters 345, 147-150.


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