Navigation Links
In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate

lation Counter and the level of bound radioactivity measured.

Results

Using the RIA method, the agonist 5-HT produced a dose-dependent inhibition of forskolin-stimulated cAMP levels with a pEC50 = 7.5 0.2 and a maximum response of 84 7% inhibition (Fig. 1a). The FlashPlate technique produced a comparable 5-HT inhibition curve with a pEC50 = 7.7 0.1 and a maximum response of 84 4% (Fig. 1b). The two assays produced similar results between other parameters, such as absolute levels of cAMP measured, and intra-assay variation (Table 1). Inter-assay variation using the FlashPlates was minimal, with only 6% error on three separate determinations of the same sample (5.07 0.33 pmol cAMP/ml). However, one obvious difference between the two methods was the time required to assay the same number of samples; the FlashPlate method saved several hours compared to the RIA method.

Discussion

To date, radioimmunoassay has been an effective method to measure cAMP within samples. It is, however, a laborious technique with few options for automation and hence not conducive for use in high throughput screening (HTS) applications. The cAMP [125I] FlashPlate Assay has overcome these drawbacks: its 96-well microplate format has proven itself highly efficacious for robotic HTS, permitting cAMP measurements to be fully automated, with improvements realized in both efficiency and accuracy. The assay kit (SMP001) is comprised of fewer components than the RIA kit, and is easier to use. Additionally, the elimination of the separation step in the FlashPlate method is a major time saving advance, and again favors full automation. The results obtained from the FlashPlates are very reproducible and comparable to those obtained from RIA, and so the former can be confidently used as an alternative to the latter. The FlashPlate method is extremely sensitive
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. In-Vitro Metabolism Studies Using Data-Dependent LC/MSn
2. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
3. Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs
4. Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs
5. RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay
6. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
7. Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Cell Proliferation and Viability Measurement
10. LabVIEW for Measurement and Data Analysis
11. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Vitro Measurement the Second Messenger cAMP RIA FlashPlate

(Date:8/3/2015)... Aug. 3, 2015  MiMedx Group, Inc. (NASDAQ: ... amniotic tissue and patent-protected processes to develop and market ... Orthopedic, Spine, Sports Medicine, Ophthalmic and Dental sectors of ... 35 th Annual Canaccord|Genuity Growth Conference in ... Chairman and CEO, William C. Taylor , President ...
(Date:8/3/2015)... Aug. 3, 2015  Aethlon Medical, Inc. (Nasdaq: AEMD ... treat life-threatening diseases, today announced that it will host ... webcast on Thursday, August 13, 2015 at 4:30 p.m. ... follow the release of fiscal 2016 first quarter results.  ... approximately two hours after the call at the Investor ...
(Date:8/3/2015)... Aug. 3, 2015 /PRNewswire/ - RepliCel Life Sciences Inc. (TSX.V: ... on the development of autologous cell therapies, announced today that ... the InvestMNT conference held in Minneapolis, Minnesota ... time. Hall,s presentation will highlight RepliCel,s 18-month milestones including CE ... Europe , clinical data from both RCT-01 (tendon) ...
(Date:7/31/2015)... , July 31, 2015 China Cord ... China,s leading provider of cord blood ... cell storage services, today announced that the Company has ... U.S. Securities and Exchange Commission. The filed Form 20-F ... March 31, 2015. The Form 20-F can be accessed ...
Breaking Biology Technology:MiMedx To Present At 35th Annual Canaccord|Genuity Growth Conference 2Aethlon Medical To Host First Quarter Fiscal 2016 Investor Call on August 13, 2015 2RepliCel Presenting at InvestMNT Investor Conference in Minneapolis 2China Cord Blood Corporation Files Its Annual Report on Form 20-F 2
... ... ... Xybion Corporation announced today that it has completed the acquisition of substantially all of ... The terms of the transactions were not disclosed., , ,Stelex and Vital-Path, based in Bensalem, ...
... CHENGDU, China , May 12 /PRNewswire-Asia-FirstCall/ -- Tianyin,Pharmaceutical ... in the modernized traditional Chinese medicine ("TCM") and branded,generics today announced ... , , Third quarter fiscal year 2010 ending ... revenue increased 60% year over year to $15.9 million, net, ...
... This release is available in French . Physicists ... energy involved in adding electrons to semi-conductor nanocrystals, also known ... and other areas of science. Dr. Peter Grtter, McGill,s Associate ... that his research team has developed a cantilever force sensor ...
Cached Biology Technology:Xybion Completes Acquisition of Stelex and Vital-Path, Leaders in Business Process Software and Consulting Services for Regulated Industries, from GE Healthcare 2Xybion Completes Acquisition of Stelex and Vital-Path, Leaders in Business Process Software and Consulting Services for Regulated Industries, from GE Healthcare 3Tianyin Reports Record Third Quarter 2010 Financial Results 2Tianyin Reports Record Third Quarter 2010 Financial Results 3Tianyin Reports Record Third Quarter 2010 Financial Results 4Tianyin Reports Record Third Quarter 2010 Financial Results 5Tianyin Reports Record Third Quarter 2010 Financial Results 6Tianyin Reports Record Third Quarter 2010 Financial Results 7Tianyin Reports Record Third Quarter 2010 Financial Results 8Tianyin Reports Record Third Quarter 2010 Financial Results 9Tianyin Reports Record Third Quarter 2010 Financial Results 10Tianyin Reports Record Third Quarter 2010 Financial Results 11Tianyin Reports Record Third Quarter 2010 Financial Results 12Tianyin Reports Record Third Quarter 2010 Financial Results 13Quantum move toward next generation computing 2
(Date:7/21/2015)... , July 21, 2015  NXT-ID, Inc. (NASDAQ: ... biometric authentication company focused on the growing mobile ... wallet, announces that it has filed provisional patent ... Method. This invention highlights next ... authorizes an account, but also the user using ...
(Date:7/20/2015)... July 20, 2015  Acuity Market Intelligence,s latest ... Convergence of Commerce and Privacy" forecasts that between ... apps will be downloaded to smart mobile devices ... biometrics market is projected to generate more than ... the seven-year forecast period.    "Biometrics ...
(Date:7/13/2015)... , Jul. 13, 2015 NXT-ID, Inc. ... a biometric authentication company focused on the growing ... filed provisional patent 62/188684 for BEHAVIORAL-DIRECTED ... another new convenient and secure method to make ... innovative payment methods, introduced with its groundbreaking voice-direct ...
Breaking Biology News(10 mins):NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 2NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 3NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 4Biometrics on Smart Mobile Devices to Redefine Digital Identity with 12.9 Billion Biometric App Downloads between 2014 and 2020 2NXT-ID Patents Behavior-Directed Payments 2NXT-ID Patents Behavior-Directed Payments 3NXT-ID Patents Behavior-Directed Payments 4
... a species of alfalfa from Siberia, to the United States. His ... But what also happened during this time in the late ... of the matter is creating a current revival in both interest ... And several researchers members of the American Society of Agronomy (ASA) ...
... 31st, 2012.- Madrid will become international destination for ... Innovation Conference 2012, focused on building high performance ... This new organization has been created by the ... Government of the Comunidad de Madrid, through the ...
... CRUZ, CA--Researchers at the University of California, Santa Cruz, ... from wounds and skin infections, using light to trigger ... developed a photoactive compound that releases nitric oxide when ... biocompatible material that could be applied as a sprayable ...
Cached Biology News:Time is ticking for some crop's wild relatives 2MADRID-MIT M+Vision Consortium hosts Biomedical Innovation Conference 2012 2Light-induced delivery of nitric oxide eradicates drug-resistant bacteria 2Light-induced delivery of nitric oxide eradicates drug-resistant bacteria 3
... Unique, compact measuring instrument for calibrating single-channel ... design and the robust carrying case, the ... in the laboratory and on-site at the ... to use because the individual channels are ...
... virus of Japan, also named Sendai virus) ... transfection of molecules by means of membrane ... (RNAi), Protein, Oligo, cDNA, Antibodies, Vector, and ... GenomONE-Neo EX has a high fusing capability ...
... The S. pombe Whole Genome ... location analysis of yeast (S. pombe) ... immunoprecipitation (ChIP) with Agilent DNA microarrays. ... reliable binding data with greater true ...
... This kit is designed for easy transformation ... protocol allows for successful transformation simply by ... reagents with cultured yeast cells. No complicated ... are required. The kit is particularly well ...
Biology Products: