Navigation Links
In Situ Cell Death Detection Kit

The In Situ Cell Death Detection Kit has been designed as a precise, fast, and simple non radioactive technique to detect and quantify apoptotic cell death at the single-cell level in tissues. It is based on the so-called TUNEL method.

During apoptosis, DNAse activity not only generates double-stranded, low-molecular-weight DNA fragments (mono- and oligonucleosomes), but also introduces strand breaks ("nicks") into the high-molecular-weight DNA. These processes can be identified by labeling the free 3-OH termini with terminal transferase (TdT), which attaches labeled nucleotides to all 3OH-ends (TUNEL reaction; TdT-mediated dUTP nick end labeling). This labeling is more sensitive than other methods, and is the method used by the Roche Applied Science In situ Cell Death Detection Kits. There are four different versions of this kit: In Situ Cell Death Detection Kit, Fluorescein; In Situ Cell Death Detection Kit, TMR red (tetramethylrhodamine red); In Situ Cell Death Detection Kit, AP (alkaline phosphatase); and In Situ Cell Death Detection Kit, POD (peroxidase).

This figure shows the detection principle of the TUNEL kits. Labeling with fluorescein may also be followed by immunohistochemical detection using anti-fluorescein-specific antibodies that are conjugated to POD or AP. Typical applications of the TUNEL kits are:
  • Detection of individual apoptotic cells in frozen and formalin-fixed tissue sections in basic research and routine pathology
  • Determination of the sensitivity of malignant cells to drug-induced apoptosis in cancer research and clinical oncology
  • Typing of cells that are undergoing cell death (in heterogeneous populations) by double-staining procedures
Typical samples that can be examined with the In Situ Cell Death Detection Kits include:
  • Cell smear preparations
  • Adherent cells cultured on chamber slides
  • Frozen, formalin-fixed, or paraffin-embedded tissue sections
The In Situ Cell Death Detection Kits, Fluorescein and TMR red, can also be used with cells in suspension (e.g., for analysis by flow cytometry).

This figure shows apoptotic cells in rabbit endometrium. The In Situ Cell Death Detection Kit, Fluorescein was used for staining a paraffin-embedded tissue section, which was then analyzed under a fluorescence microscope.



Page: All 1 2

Related biology technology :

1. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
2. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
3. A New PCR-based Mycoplasma Detection Method
4. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
5. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
6. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
7. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
8. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
9. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer
Post Your Comments:

(Date:10/12/2017)... ... October 12, 2017 , ... DuPont Pioneer and recently formed ... entered into a multiyear collaboration to identify and characterize novel CRISPR-Cas nucleases. The ... gene editing across all applications. , Under the terms of the agreement, Pioneer ...
(Date:10/12/2017)... ... October 12, 2017 , ... ... with the addition of its newest module, US Hemostats & Sealants. , SmartTRAK’s ... hemostats, absorbable hemostats, fibrin sealants, synthetic sealants and biologic sealants used in surgical ...
(Date:10/12/2017)... ... October 12, 2017 , ... ... first-ever genomics analysis platform specifically designed for life science researchers to analyze ... pioneering researcher Rosalind Franklin, who made a major contribution to the discovery ...
(Date:10/11/2017)... ... October 11, 2017 , ... The ... endogenous context, enabling overexpression experiments and avoiding the use of exogenous expression plasmids. ... is transformative for performing systematic gain-of-function studies. , This complement to loss-of-function ...
Breaking Biology Technology:
(Date:4/13/2017)... 2017 According to a new market research report ... Identity Administration, and Authorization), Service, Authentication Type, Deployment Mode, Vertical, and Region ... expected to grow from USD 14.30 Billion in 2017 to USD 31.75 ... ... MarketsandMarkets Logo ...
(Date:4/6/2017)... April 6, 2017 Forecasts by ... Document Readers, by End-Use (Transportation & Logistics, Government & ... Gas & Fossil Generation Facility, Nuclear Power), Industrial, Retail, ... Are you looking for a definitive report ... ...
(Date:4/3/2017)... 2017  Data captured by IsoCode, IsoPlexis ... a statistically significant association between the potency ... and objective response of cancer patients post-treatment. ... whether cancer patients will respond to CAR-T ... as to improve both pre-infusion potency testing and ...
Breaking Biology News(10 mins):