Navigation Links
In Situ Cell Death Detection Kit

The In Situ Cell Death Detection Kit has been designed as a precise, fast, and simple non radioactive technique to detect and quantify apoptotic cell death at the single-cell level in tissues. It is based on the so-called TUNEL method.



During apoptosis, DNAse activity not only generates double-stranded, low-molecular-weight DNA fragments (mono- and oligonucleosomes), but also introduces strand breaks ("nicks") into the high-molecular-weight DNA. These processes can be identified by labeling the free 3-OH termini with terminal transferase (TdT), which attaches labeled nucleotides to all 3OH-ends (TUNEL reaction; TdT-mediated dUTP nick end labeling). This labeling is more sensitive than other methods, and is the method used by the Roche Applied Science In situ Cell Death Detection Kits. There are four different versions of this kit: In Situ Cell Death Detection Kit, Fluorescein; In Situ Cell Death Detection Kit, TMR red (tetramethylrhodamine red); In Situ Cell Death Detection Kit, AP (alkaline phosphatase); and In Situ Cell Death Detection Kit, POD (peroxidase).



This figure shows the detection principle of the TUNEL kits. Labeling with fluorescein may also be followed by immunohistochemical detection using anti-fluorescein-specific antibodies that are conjugated to POD or AP. Typical applications of the TUNEL kits are:
  • Detection of individual apoptotic cells in frozen and formalin-fixed tissue sections in basic research and routine pathology
  • Determination of the sensitivity of malignant cells to drug-induced apoptosis in cancer research and clinical oncology
  • Typing of cells that are undergoing cell death (in heterogeneous populations) by double-staining procedures
Typical samples that can be examined with the In Situ Cell Death Detection Kits include:
  • Cell smear preparations
  • Adherent cells cultured on chamber slides
  • Frozen, formalin-fixed, or paraffin-embedded tissue sections
The In Situ Cell Death Detection Kits, Fluorescein and TMR red, can also be used with cells in suspension (e.g., for analysis by flow cytometry).



This figure shows apoptotic cells in rabbit endometrium. The In Situ Cell Death Detection Kit, Fluorescein was used for staining a paraffin-embedded tissue section, which was then analyzed under a fluorescence microscope.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
2. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
3. A New PCR-based Mycoplasma Detection Method
4. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
5. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
6. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
7. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
8. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
9. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
10. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
11. Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/29/2016)... ... ... Intelligent Implant Systems announced today that the two-level components for the Revolution™ Spinal ... These components expand the capabilities of the system and allow Revolution™ to be ... 2015, the company has seen significant sales growth in 1Q 2016, and the system ...
(Date:4/28/2016)... ... April 28, 2016 , ... Next ... a talk on its first-in-class technologies for tissue stem cell counting and expansion ... RNAiMicroRNA Biology to Reprogramming & CRISPR-based Genome Engineering in Burlington, Massachusetts. , The ...
(Date:4/27/2016)... (PRWEB) , ... April 27, 2016 , ... The Pittcon ... Pittcon 2019. Chuck has been a volunteer member of Committee since 1987. Since ... the board of directors and treasurer and was chairman for both the program and ...
(Date:4/27/2016)... , ... April 27, 2016 , ... ... Jon Clark has joined the company as an Expert Consultant. Mr. Clark ... industry collaborations and managing the development of small molecule monographs based on analytical ...
Breaking Biology Technology:
(Date:3/29/2016)... 29, 2016 LegacyXChange, Inc. (OTC: ... and SelectaDNA/CSI Protect are pleased to announce our successful ... a variety of writing instruments, ensuring athletes signatures against ... collectibles from athletes on LegacyXChange will be assured of ... DNA. Bill Bollander , CEO states, ...
(Date:3/18/2016)... LONDON , March 18, 2016 ... Established Suppliers of Biometrics, ICT, Manned & Unmanned Vehicles, Physical ... & security companies in the border security market and ... and Europe has led ... your companies improved success. --> defence & ...
(Date:3/14/2016)... http://www.apimages.com ) - ... - Renvoi : image disponible via AP Images ( ... --> DERMALOG, le leader de l,innovation ... d,empreintes digitales pour l,enregistrement des réfugiés en Allemagne. ... produire des cartes d,identité aux réfugiés. DERMALOG dévoilera ...
Breaking Biology News(10 mins):