Navigation Links
Improving RNA Amplification

bation times and more reliable access to expression information from total RNA samples less than 1 g. The increase also translates through to two rounds of amplification, allowing more robust amplification of total RNA from 10 ng to as low as 100 pg.


Expectations for Yields
Factors such as tissue source, mRNA content (0.1-3%), starting amount of total RNA, and the quality of RNA used, will all affect the yield of cRNA. Collectively these factors can lead to up to 4-fold variation in yields. Figure 2 provides typical yields of cRNA amplified from six different sources using the MessageAmp II aRNA Amplification Kit. RNA inputs ranging from 50-3000 ng were tested from each source. From Figure 2 one can calculate the level of amplification obtained from different tissues at different inputs. The figure is also a useful benchmark for understanding the amount of cRNA produced both from different total RNA inputs and sources.

Figure 2. MessageAmp II aRNA Amplification Yields. Yields of cRNA (g) amplified using the MessageAmp II aRNA Amplification Kit from six different RNA sources using total RNA inputs of 50-3000 ng. Yields shown are the average of duplicate reactions. The in vitro transcription reaction incubation time was 4 hours for all samples. Purified cRNA concentrations were measured using a NanoDrop spectrophotometer.
*Universal Human Reference RNA (Stratagene)


One or Two Round Amplification?
Based on specific experimental constraints, researchers must decide whether one or two rounds of amplific
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Related biology technology :

1. Improving the Efficiency of Common Filtration Tasks in your Laboratory
2. Improving an Integral Part of the Lab: Vacuum
3. Improving the accuracy and speed of mammalian cell counting
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
6. Greater Amplification Specificity with New Hot Start PCR Enzyme
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
9. Improve Amplification Specificity with Hot Start PCR Enzyme
10. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
11. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Improving RNA Amplification

(Date:10/22/2014)... Massachusetts (PRWEB) October 22, 2014 ... announced the release of a new infographic, Understanding ... to create a beautifully designed, easy to follow informative ... transmission, areas of outbreak, symptoms and prevention. , "As ... discovered that the 2014 Ebola outbreak represents not only ...
(Date:10/22/2014)... According to new market research report "Cloud Professional ... & Support), by Service Model (SaaS, PaaS, IaaS), ... & Community)) - Global Forecast to 2019", published ... Services Market into various sub-segments with an in-depth ... the drivers and restraints for this market with ...
(Date:10/22/2014)... HONG KONG , Oct. 22, 2014 ... enterprise, announced today that rare disease expert John ... president, research. Dr. McKew brings more than two decades ... at the National Institutes of Health, Wyeth Research and ... McKew will lead aTyr,s efforts to expand and translate ...
(Date:10/20/2014)... ON , Oct. 20, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: ... $1.7 million which brings Q3 orders to $5.8 million and provides ... control projects in North America and one ... continue to bid new projects at record levels," said Peter ... were higher at the end of Q3 than they have been ...
Breaking Biology Technology:Involution Studios' Ebola Infographic Provides Key Disease Information and Statistics at a Glance 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 4Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 5aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3
... Corp. Ltd., a developer and marketer of innovative products ... today announced that Gary Levine has been appointed Chief ... has joined the company as Director of Reagents Development ... Martin, ZyGEM is developing an innovative Rapid DNA analysis ...
... have created a working cloaking device that not only ... but also boasts unique features; it has an ,on ... The researchers, from the University of Dallas, Texas, ... in a fascinating video shown here http://www.youtube.com/watch?v=3YO4TTpYg7g ...
... Agendia, an innovative molecular cancer diagnostics company, ... routine inspection of its Irvine, California-based laboratories by the ... 510(k) clearance for its MammaPrint® breast cancer recurrence test ... test of its kind to receive FDA clearance, which, ...
Cached Biology Technology:ZyGEM Strengthens Senior Management Team With New Appointments 2ZyGEM Strengthens Senior Management Team With New Appointments 3'Mirage-effect' helps researchers hide objects 2Agendia Successfully Completes Bi-Annual FDA Inspection 2
(Date:10/15/2014)... admitted Johannes Gutenberg University Mainz (JGU) as a ... continues to maintain a significant nationwide standing in ... acceptance of Johannes Gutenberg University Mainz as a ... in the national and international competition for the ... Rhineland-Palatinate can be proud that Mainz University has ...
(Date:10/15/2014)... Conn. , Oct. 15, 2014 NXT-ID, Inc. (NASDAQ: ... biometric authentication company focused on the growing mobile commerce market releases ... MarketSite in Times Square on Monday October 13 th . ... CTO David Tunnel and angel investor Mr. Chad ... Pereira , CEO of NXT-ID thanked his investors and employees "for ...
(Date:10/14/2014)... – Like discriminating thieves, prostate cancer tumors scavenge and ... body. But such avarice may be a fatal weakness. ... way to kill prostate cancer cells by delivering a ... destroys the diseased cells brimming with the mineral, leaving ... two drugs already commercially available for other uses, could ...
Breaking Biology News(10 mins):Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 2Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 3Photo Release: NXT-ID Inc. Rings Opening Bell of the NASDAQ Stock Market October 13th 2Photo Release: NXT-ID Inc. Rings Opening Bell of the NASDAQ Stock Market October 13th 3Prostate cancer's penchant for copper may be a fatal flaw 2
... platform, complete with biometric enrollment, authentication, data ... formatting, and background check connectivity, BEDFORD, ... (Nasdaq: AWRE ), a global provider of biometrics ... with Sun identity management solutions. The integration of,BioSP with ...
... Natural selection . . . By examining ... Oak Ridge National Laboratory hope to develop a strategy ... a process known as association mapping, scientists will attempt ... growing in natural populations with the goal being to ...
... research team in Antarctica led by David Marchant, ... University, has reported the discovery of exceptionally well-preserved ... as diatoms, small fresh water crustaceans, and insects ... the southernmost region of the continent before a ...
Cached Biology News:Sun Microsystems and Aware, Inc. Announce Integration of Aware's BioSP with Sun Identity Management Solutions 2Sun Microsystems and Aware, Inc. Announce Integration of Aware's BioSP with Sun Identity Management Solutions 3Sun Microsystems and Aware, Inc. Announce Integration of Aware's BioSP with Sun Identity Management Solutions 4Sun Microsystems and Aware, Inc. Announce Integration of Aware's BioSP with Sun Identity Management Solutions 5Story tips from the US Department of Energy's Oak Ridge National Laboratory, August 2008 2Story tips from the US Department of Energy's Oak Ridge National Laboratory, August 2008 3Rare Antarctic fossils reveal extinction of tundra before full polar climate arrived 2Rare Antarctic fossils reveal extinction of tundra before full polar climate arrived 3
Recombinant Viral CCI/Fc Chimera, CF...
... Pig serum is collected from fasted Guinea Pigs. ... Citrate N-04: Heparin (Na) N-06: ... N-10: EDTA (Na) N-03: Alsevers ... N-09: K3EDTA N-11: Heparin ...
GENOMIPHI V2 DNA AMP KIT 25RXN, 1 EA. Category: Genomics DNA Amplification....
EPH electrode, anode, 1. Category: Electrophoresis Systems & Standards & Reagents, IEF & SDS-PAGE & 2-D Electrophoresis, Multiphor II Kits & Components and Accessories....
Biology Products: