Navigation Links
Improving RNA Amplification

based aRNA amplification kit. The MessageAmp II cRNA Amplification Kit incorporates extensive improvements to the original MessageAmp procedure. In developing MessageAmp II, Ambions scientists focused on the reverse transcription and second strand cDNA synthesis steps, both of which are critical for generating high yields of full-length labeled cRNA. This led to the development of ArrayScript, an engineered M-MLV reverse transcriptase, and to the optimization of the second strand cDNA synthesis reaction to synergize with the improvements gained with ArrayScript. One of the principal benefits conferred by ArrayScript and the optimized second-strand synthesis step is that MessageAmp II will generate sufficient labeled cRNA in a single round of amplification for array analysis from as little as 100 ng of total RNA (Figure 1). Other commercially available products for cRNA amplification usually demand two rounds of amplification at this level of input.

Figure 1. High Density Oligonucleotide Microarray Hybridized with MessageAmp II cRNA. Shown is a portion of a GeneChip array image used in this report. All RNA samples were amplified and labeled with biotin-16-UTP/-11-CTP (PerkinElmer Life Sciences), with the MessageAmp II Kit. Biotin labeled cRNA was fragmented, then hybridized and stained using GeneChip Human Genome Focus Arrays following the GeneChip Expression Analysis technical manual.


Together, the RT and second strand cDNA reactions result in an increase in the conversion of mRNA into longer double-stranded cDNA templates. This increase allows both shortened in vitro transcription (IVT) reaction incu
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Related biology technology :

1. Improving the Efficiency of Common Filtration Tasks in your Laboratory
2. Improving an Integral Part of the Lab: Vacuum
3. Improving the accuracy and speed of mammalian cell counting
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
6. Greater Amplification Specificity with New Hot Start PCR Enzyme
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
9. Improve Amplification Specificity with Hot Start PCR Enzyme
10. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
11. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Improving RNA Amplification

(Date:4/27/2015)... (PRWEB) April 27, 2015 tru Shrimp™ ... agreement with Texas A&M University for the exclusive worldwide ... as super-intensive raceway systems. The license agreement for the ... Lawrence, extends to countries such as China, Taiwan, France, ... , “We have had inquires from all over ...
(Date:4/24/2015)... April 24, 2015 Top student social ... San Diego area universities, including UCSD and SDSU, will ... from prominent local, national and nonprofit companies for an ... 5th Annual Social Innovation Challenge 2015 . , ... social venture ideas addressing a diverse range of issues ...
(Date:4/24/2015)... 24, 2015  Pfenex Inc. (NYSE MKT: PFNX) a ... therapeutics including high value and difficult to manufacture proteins, ... of 6,000,000 shares of its common stock at a ... the shares being offered, 2,610,000 are being offered by ... by existing stockholders. Pfenex will not receive any proceeds ...
(Date:4/23/2015)... 2015 Ralco announced today that it ... (ABN) for the exclusive worldwide rights to sell and ... is a new in-line milk system that increases the ... to move supplementation milk cups within a farrowing facility ... technology lets producers get Birthright™ milk to the right ...
Breaking Biology Technology:tru Shrimp™ Systems Acquires Global Patent for Tidal Basin™ Shrimp Production 25th Annual Social Innovation Challenge at University of San Diego - Students Pitch to Live Judging Panel to Take Home $75K 25th Annual Social Innovation Challenge at University of San Diego - Students Pitch to Live Judging Panel to Take Home $75K 3Pfenex Inc. Announces Pricing Of Follow-on Offering 2Ralco Acquires License to Birthright™ Moveable Milk Cup 2
... and Funding Barriers and Paves Way ... for Affordable and Efficient Approach, KAKAMEGA, Kenya, ... and HIV was launched today in the Western,Kenyan district of ... a basic care package consisting of a,PermaNet(R) long-lasting insecticide-treated bed ...
... Institutional Investors to Attend Conference, MONTREAL, Sept. ... today announced that Mr. Pierre Laurin, President,and Chief ... 5th,annual Investor Summit on September 16, 2008 at ... Mark Hopkins InterContinental Hotel in San Francisco., ...
... Calif., Sept. 12 Acologix, Inc., a privately,held ... studies demonstrating that AC-100, its therapeutic product,candidate derived ... and,dental cells, promotes cartilage regeneration in large animal ... A. Middleton-Hardie on September,13, 2008 at the 30th ...
Cached Biology Technology:Integrated Prevention Demonstration Campaign Launched in Western Kenya to Fight HIV, Malaria and Diarrhoeal Disease 2Integrated Prevention Demonstration Campaign Launched in Western Kenya to Fight HIV, Malaria and Diarrhoeal Disease 3ProMetic to present at Merriman Curhan Ford's Investor Summit 2008 on September 16th 2Acologix Presents Preclinical Data on Cartilage Regeneration by AC-100 at the 30th ASBMR Annual Meeting 2Acologix Presents Preclinical Data on Cartilage Regeneration by AC-100 at the 30th ASBMR Annual Meeting 3
(Date:3/24/2015)... ( http://www.researchandmarkets.com/research/kvdtcw/face_and_voice ) has announced the addition of the ... report to their offering. This report analyzes the worldwide ... following Segments: Face Biometrics, and Voice Biometrics. The report provides separate ... Japan , Europe , ... Africa , and Latin America . ...
(Date:3/23/2015)... YORK , March 23, 2015   HOYOS ... property management company, today announced that the Company will ... for both enterprise and consumers at Connect:ID on March ... HOYOS Labs will highlight the IEEE Biometric ... TM ; and enterprise access control system. BOPS ...
(Date:3/20/2015)... --Research and Markets ( http://www.researchandmarkets.com/research/rswjb4/hand_geometry ) has announced ... Business Report" report to their offering. This ... Thousands. The report provides separate comprehensive analytics for the US, ... Europe , Asia-Pacific , ... Annual estimates and forecasts are provided for the ...
Breaking Biology News(10 mins):Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 3Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 4Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 5Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 6HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 2Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 4
... presented Jim Gartland of the Virginia Institute of Marine ... for his outstanding contributions to the field of fisheries ... Atlantic coastal states in 1942 for the conservation and ... covers near-shore waters from Florida to Maine. ASMFC ...
... NYNovember 6, 2013: Decades worth of research shows that teathe ... illnesses, including heart disease, certain types of cancer and type ... help promote weight loss and maintain a healthy weight, improve ... attention, problem solving and mood. The December 2013 issue ...
... suggests that the brain plays a key role in glucose regulation ... Nov. 7 issue of the journal Nature . If the ... ways to prevent and treat this disease, which is projected to ... In the paper, lead author Dr. Michael W. Schwartz, director ...
Cached Biology News:ASMFC honors Gartland with Annual Award of Excellence 2ASMFC honors Gartland with Annual Award of Excellence 3New research shows tea may help promote weight loss, improve heart health and slow progression of prostate cancer 2New research shows tea may help promote weight loss, improve heart health and slow progression of prostate cancer 3New research shows tea may help promote weight loss, improve heart health and slow progression of prostate cancer 4New research shows tea may help promote weight loss, improve heart health and slow progression of prostate cancer 5Brain may play key role in blood sugar metabolism and development of diabetes 2
One-step, microplate or cuvet, colorimetric, detection range 0.1 mg/dL to 3 mg/dL. Procedure: 10 min....
Request Info...
Recombinant Mouse Wnt-3a...
... at least 20 structurally and functionally related ... superfamily. BMPs were originally identified as protein ... are also involved in embryogenesis and morphogenesis ... the growth, differentiation, chemotaxis and apoptosis of ...
Biology Products: