Navigation Links
Improving RNA Amplification

alize that all methods will have some inherent bias [8]. Some of this bias can be minimized with a robust protocol and optimized reagents. The MessageAmp II aRNA Amplification Kit provides such a protocol and the novel reverse transcriptase (ArrayScript) for this purpose. We attempted to monitor correlation and standard deviation between two important factors, amplification protocol and RNA input. The data indicate that the MessageAmp II protocol is highly reproducible and can be meaningfully compared to the Affymetrix standard protocol and the original MessageAmp protocol (see sidebar, MessageAmp II Concordance with Original MessageAmp Kit). We hope to have provided some guidelines for amplification expectations and confidence that a shortened IVT incubation time that produces enough cRNA for a microarray will not significantly effect expression data (compared to longer IVT times). The above examples have been an important part of our research, production, and quality control development for procedures that we use to maintain the highest quality reagents for microarray analysis. We plan on continuing such studies for both new product development and improving our line of RNA amplification products.


Affymetrix and GeneChip are registered trademarks owned or used by Affymetrix, Inc.


back to top


Ordering Information
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Related biology technology :

1. Improving the Efficiency of Common Filtration Tasks in your Laboratory
2. Improving an Integral Part of the Lab: Vacuum
3. Improving the accuracy and speed of mammalian cell counting
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
6. Greater Amplification Specificity with New Hot Start PCR Enzyme
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
9. Improve Amplification Specificity with Hot Start PCR Enzyme
10. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
11. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Improving RNA Amplification

(Date:7/29/2014)... 29, 2014 Human Longevity, Inc. (HLI), a ... on extending the healthy, high performance human life span, ... Ph.D., an expert in machine learning and machine translation ... HLI from Google where he was Distinguished Research Scientist ... directly to HLI Co-Founder and CEO, J. Craig ...
(Date:7/29/2014)... , July 29, 2014  Pomerantz LLP is investigating ... ("Impax" or the "Company") (NASDAQ: IPXL).  Such investors are ... rswilloughby@pomlaw.com or 888-476-6529, ext. 237. ... officers and/or directors have violated Sections 10(b) and 20(a) ... On July 29, 2014, Impax Laboratories, Inc. (the ...
(Date:7/29/2014)... Oregon , July 29, 2014 ... "Global surgical equipment market (types, applications and geography) - ... Research, Report, Opportunities, Segmentation and Forecast, 2013 - 2020", ... to reach $12.1 billion by 2020 registering a CAGR ... to the breakthrough achieved by the electro surgical devices ...
(Date:7/29/2014)... July 29, 2014 Philadelphia’s newest entrepreneurial incubation ... get their start. The Innovation Center @3401 , a ... University City Science Center to help attract and nurture ... Monday, July 28. , The Innovation Center ... and Market Streets, is the new home of DreamIt ...
Breaking Biology Technology:Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 2Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 3SHAREHOLDER ALERT: Pomerantz Law Firm Investigates Claims On Behalf of Investors of Impax Laboratories, Inc. - IPXL 2Global Surgical Equipment Market is expected to reach $12.1 billion by 2020 - Allied Market Research 2Global Surgical Equipment Market is expected to reach $12.1 billion by 2020 - Allied Market Research 3Innovation Center @3401 Opens for Business; DreamIt Ventures and Point.io First Residents 2Innovation Center @3401 Opens for Business; DreamIt Ventures and Point.io First Residents 3
... Global consultant Tom Koulopoulos said at the Fusion2006 CEO-CIO ... work they do that does not support their core competency ... expect, a discussion of outsourcing versus doing work in house ... the Delphi Group founder to tap his experience in IT ...
... Robert Paulos, associate director for the IceCube project, holds ... IceCube neutrino telescope. Photo courtesy of the Ice Cube ... of scientists and engineers has taken a major step ... cosmic neutrino observatory, harnessing a sophisticated hot-water drill to ...
... have been infected with the H5N1 avian influenza virus, mostly ... virus does not spread easily from its pioneering human hosts ... a study of cells in the human respiratory tract reveals ... that makes it difficult for the virus to jump from ...
Cached Biology Technology:IT outsourcing according to Tom Koulopoulos, part 1 2IT outsourcing according to Tom Koulopoulos, part 1 3Polar neutrino observatory construction leaps ahead 2Polar neutrino observatory construction leaps ahead 3Polar neutrino observatory construction leaps ahead 4UW shows why avian flu hasn't spread among humans yet 2UW shows why avian flu hasn't spread among humans yet 3
(Date:7/28/2014)... of a new study suggest two ways to effectively address ... well in obese women, compared to women of a normal ... method, researchers say, but as the population has increased in ... for obese women. Studies have consistently found that obesity has ... may in turn affect how well the pill prevents pregnancy. ...
(Date:7/28/2014)... himself the bug hunter, but the target of his ... and identified with special methods and instruments. Benjamin Hause, ... Diagnostic Laboratory at Kansas State University, recently published an ... which is an important find in the United States. ... didn,t know what it was," Hause said. "We used ...
(Date:7/28/2014)... HOUSTON (July 28, 2014) Rice University researchers ... chains of varying flexibility that can be used as ... visual proof that "bead-spring" polymers, introduced as theory in ... flexible as required and should be of interest to ... , The work led by Rice chemical and biomolecular ...
Breaking Biology News(10 mins):Strategies identified to improve oral contraceptive success with obese women 2Researcher using next-generation sequencing to rapidly identify pathogens 2Researcher using next-generation sequencing to rapidly identify pathogens 3Seeing is bead-lieving 2
... new gene could lead to better bug-resistant plants. ... the cover of this week,s Proceedings of the ... could re-learn a thing or two from their wild ... losing beneficial traits common to wild tomatoes. Anthony Schilmiller, ...
... Sept. 17, 2012Whether your neighbourhood is conducive to ... according to a new study by researchers at ... Evaluative Sciences. Researchers found this risk was ... neighbourhoods. A new immigrant living in a less ...
... study suggests there are two root causes of a type ... already have sparked a clinical trial, may guide development of ... CF. Almost half of patients with CF will ... develop it in their teens. In addition to the health ...
Cached Biology News:New gene could lead to better bug-resistant plants 2Risk of developing diabetes higher in neighborhoods that aren't walk-friendly: Study 2Cystic fibrosis disrupts pancreas two ways in CF-related diabetes 2Cystic fibrosis disrupts pancreas two ways in CF-related diabetes 3
... Agilents G1979A multisignal output accessory ... multiple data systems in laboratories with ... unprocessed digital MS signals from Agilent ... into analog format and transmits the ...
... DSQ II (Dual-Stage Quadrupole) Thermo Scientific ... leader in analytical instrumentation, introduces the latest ... DSQ II. Based on the proven reliability ... PolarisQ GC/MS systems, the DSQ II incorporates ...
... the macro- and micronutrients as ... (1962) and the vitamins as ... (1968). Preparation Quantity Equivalency: Formulated ... powder per liter of medium. ...
... Contents include: Microarray hybridization chamber ... chamber top, clamp, thumbscrew) Microarray chamber user ... and are intended to be cleaned and ... with Agilents Catalog Oligo Microarray kits and ...
Biology Products: