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Improving RNA Amplification

Performance of New MessageAmp II on High Density Oligonucleotide Microarrays


New ArrayScript, an M-MLV RT engineered to maximize yields of full-length cDNA

Amplify as little as 100 ng of input RNA in a single round for GeneChip analysis

cRNA synthesis powered by MEGAscript, the best technology for in vitro transcription

High labeling efficiency yielding consistently high percent Present calls, and low 3'/5' ratios in GeneChip analysis


RNA amplification is often required for producing sufficient amounts of labeled target nucleic acid for detection by array hybridization. The original T7 RNA polymerase linear amplification method described and patented by Van Gelder and Eberwine [1-3] is the basis for sample preparation for most commercial microarray platforms, including the high quality Affymetrix GeneChip. At Ambion we recognize that there is more to improving RNA amplification than simply increasing yields of labeled cRNA. We therefore, set out to ensure that any observed improvements in amplification coincide with accepted standards for high quality expression profiling.


RNA Amplification Enhancements
Ambion is recognized as one of the key research companies for development of comprehensive solutions for cRNA (aRNA) amplification. Ambions MessageAmp aRNA Amplification Kit was the first commercially available Eberwine-
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