( binding domain of the yeast GAL4. When ...)Improved Vectors for PathDetect Trans-Reporting Systems

binding domain of the yeast GAL4. When a fusion trans-activator plasmid, reporter plasmid and gene of interest are cotransfected into mammalian cells, either direct or indirect phosphorylation of the fusion trans-activator protein by the uncharacterized gene product will cause transcription of the luciferase gene from the reporter plasmid. Luciferase activity, which can be conveniently assayed using Stratagenes Luciferase Assay Kit, is an indication that the gene of interest is involved in the pathway being evaluated.

Recently, Stratagene expanded the PathDetect reporting systems by introducing the pFA-ATF2 and pFA-cFos fusion trans-activator plasmids⁸ for studying signal transduction pathways that converge at the transcription factors ATF2 and c-Fos, respectively. At the same time, Stratagene introduced the pFA-CMV vector.⁹ This special vector can be used for easy cloning of the activation domain of any transcription factor sequence and subsequent expression of GAL4 fusion proteins in a variety of mammalian cells. The pFA-CMV vector features a multiple cloning site with 10 unique, conveniently arranged restriction sites for insertion of any activation domain sequence; the CMV promoter for high-level expression of fusion trans-activator proteins in many mammalian cell lines and the neomycin-resistance gene, which facilitates selection of stable cell lines that express fusion trans-activator proteins.

Improved Fusion Trans-Activator Plasmids

To upgrade the PathDetect reporting systems, Stratagene is replacing the pFA-cJun, pFA-CREB and pFA-Elk1 plasmids with improved fusion trans-activator plasmids. The new pFA2-cJun, pFA2-CREB and pFA2-Elk1 plasmids were derived from the pFA-CMV vector and offer expression driven by the powerful CMV promoter and the capability for G418 selection of stable cell lines. A new negative control plasmid, the pFC2-dbd plasmid, completes this se
'"/>

Source:

Page: All 1 2 3 4 5
Related biology technology :

1. The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature
2. Improved Sensitivity for Staining RNA with SYBR Gold Stain
3. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
4. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
5. The Worlds Best DNase Improved TURBO DNA-free
6. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
7. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
8. Epitope-Tagging Vectors for Functional Analysis in Yeast
9. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
10. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
11. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors