Navigation Links
Improved Vectors for PathDetect Trans-Reporting Systems

In vivo readout of signal transduction pathway activation

Li Xu Tim Sanchez Mary Buchanan Chao-Feng Zheng
Stratagene

Stratagenes PathDetect in vivo signal transduction pathway reporting systems can be used to assess the in vivo activation of signal transduction pathways. To expand the utility of these systems, Stratagene is replacing the pFA-cJun, pFA-CREB and pFA-Elk1 plasmids with new, improved fusion trans-activator plasmids. The pFA2-cJun, pFA2-CREB and pFA2-Elk1 plasmids feature the cytomegalovirus (CMV) promoter for expression of fusion proteins in a wide variety of cell lines and the neomycin-resistance gene for facilitating selection of stable cell lines.

Stratagene recently introduced the PathDetect in vivo signal transduction pathway trans-reporting systems, which are designed for specific, rapid and convenient assessment of the in vivo activation of signal transduction pathways.1 Researchers are successfully using these reporting systems for studying the in vivo effects of new genes, growth factors, drug candidates and extracellular stimuli on the activation of c-Jun N-terminal kinase2,3 (JNK), mitogen-activated protein kinase4,5 (MAPK), cyclic AMP-dependent protein kinase6,7 (PKA) and other signaling molecules leading to the activation of these kinases. Each PathDetect reporting system includes (1) a pathway-specific fusion trans-activator plasmid, which is an in-frame fusion of an activation domain and the DNA binding domain of the yeast trans-activator GAL4,6,8 (2) the pFR-Luc reporter plasmid for expression of the Photinus pyralis (firefly) luciferase gene controlled by a synthetic promoter that contains the yeast GAL4 upstream activating sequences (UAS) (3) a positive control plasmid that is known to activate the trans-activator protein and (4) a negative control plasmid that contains only the DNA
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature
2. Improved Sensitivity for Staining RNA with SYBR Gold Stain
3. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
4. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
5. The Worlds Best DNase Improved TURBO DNA-free
6. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
7. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
8. Epitope-Tagging Vectors for Functional Analysis in Yeast
9. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
10. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
11. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Improved Vectors for PathDetect Trans Reporting Systems

(Date:12/19/2014)... MA (PRWEB) December 18, 2014 LayerBio ... for ophthalmology and wound care. The National Eye Institute ... awarded LayerBio a Phase I SBIR grant to develop ... Cataracts are the most common cause of vision loss ... of blindness worldwide. According to Dr. Ken Mandell, LayerBio’s ...
(Date:12/19/2014)... (PRWEB) December 18, 2014 iLab Solutions, ... the appointment of Siri Bryant as the new Director ... Director of Core Implementations. These two new leadership positions ... customer service by ensuring that iLab continues to meet ... the last four years, iLab has been deployed at ...
(Date:12/19/2014)... December 18, 2014 With only 2 ... special on Sartorius Biohit products . The product ... pipettes, Picus Electronic Pipette Trade-in Program, and free pipette ... Safety officers around the world. They are ideal for ... features include:, ,     Full volume range of ...
(Date:12/19/2014)... Dec. 18, 2014   ISN has released ... Performance Indicators publication series. With data aggregated ... reported in 2013 within its online contractor management platform, ... key performance indicator (KPI) statistics. The ... publication is available in two industry specific ...
Breaking Biology Technology:LayerBio Awarded NIH Grant to Develop a Drug Delivery Device for Cataract Surgery 2iLab Solutions Announces Two New Leadership Positions to Meet the Needs of Its Growing Customer Base 2Major Pipette Distributor Pipette.com Announces Sartorius Year End Specials 2ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 2ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 3
... 16 Scientists at Biomodels have successfully ... treatment-relat e d fatigue (CTRF). The ... independent of anemia or weight loss and persists long after cancer ... will be presented today at the American Association for Cancer Research-NCI-EORTC ...
... Nov. 16 Neogen Corporation (Nasdaq: NEOG ) today announced ... With the split, shareholders of record on Nov. 30, 2009, will ... , The stock split will be effected in the form ... common stock on Dec. 15, 2009. As of today, Neogen has ...
... 16 Cornerstone Therapeutics Inc. (Nasdaq CM: CRTX), a ... significant products primarily for the respiratory and related markets, ... Chief Financial Officer, will be presenting a corporate overview ... at 9:00AM EST on Wednesday, November 18, 2009 ...
Cached Biology Technology:Biomodels Scientists Report Development of a Novel Model for Cancer Treatment-Related Fatigue 2Neogen Announces 3 for-2 Stock Split 2Cornerstone Therapeutics to Present at the 6th Annual Lazard Capital Markets Healthcare Conference 2
(Date:12/11/2014)... Dec. 09, 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has announced the ... 2015-2019" report to their offering. ... is the adoption of multimodal biometric systems. Multimodal ... an individual for verification and identification purposes. This ...
(Date:12/10/2014)... , Dec. 9, 2014  Wake Forest Baptist Medical ... building for its School of Medicine. Funding for this ... capital campaign that will be publicly launched next summer. ... located in the former 60 series R.J. Reynolds Tobacco ... Quarter. Construction will begin immediately with plans to be ...
(Date:12/5/2014)... Utah , Dec. 4, 2014  Tute Genomics, ... raised $2.3 million in Series A1 funding led by ... investors also participated in the investment round. ... the healthcare community adopts next-generation sequencing and seeks new ... said Reid Robison , MD MBA, and CEO ...
Breaking Biology News(10 mins):Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3
... Mere months after porcine epidemic diarrhea virus (PEDV) ... University of Minnesota researchers have developed a PEDV rapid ... available now, provides a way to quickly and cost-effectively ... Characterized by acute diarrhea and vomiting, a PEDV outbreak ...
... (NASDAQ: AWRE ), a leading supplier of biometrics ... reported financial results for its second quarter ended June 30, ... 2013 was $4.9 million, an increase of 19% compared to ... for the second quarter of 2013 was $307,000, or $0.01 ...
... weight should eat high fiber, low-fat food balanced with ... still burning fat, according to a report by an ... has a record number of overweight athletes, a population ... Nationally, more than 45 percent of high school linebackers ...
Cached Biology News:U of M researchers unveil nation's first porcine virus rapid detection test 2Aware, Inc. Reports Second Quarter 2013 Financial Results 2Aware, Inc. Reports Second Quarter 2013 Financial Results 3Aware, Inc. Reports Second Quarter 2013 Financial Results 4Aware, Inc. Reports Second Quarter 2013 Financial Results 5Athletes need to be careful to monitor diet, weight to maintain muscle mass 2Athletes need to be careful to monitor diet, weight to maintain muscle mass 3
... unit with motorized heated lid and skirted inner ... for microarrays and chips. The four screw-down points ... force of the motorized lid is set with ... results. This interchangeable Moto Alpha unit functions on ...
... These skirted micro test tubes are ... of polypropylene, these tubes have uniform walls ... nonsterile, with separate O-ring screw caps. The ... and have an operating range from -55 ...
... carriers to suit the widest possible range ... science laboratory. Labware carriers can be placed ... is simple for the user, thanks to ... are correctly guided into position. This allows ...
... For affinity chromatography, Affi-Prep protein ... (IgG), selectively remove IgG prior to ... adsorb immune complexes for antigen purification. ... region of immunoglobulins, especially IgG from ...
Biology Products: