Navigation Links
Improved Quantitative Selectivity of Clenbuterol in Human Urine using ,,, High Resolution on the TSQ Quantum Mass Spectrometer

ion Analysis, in SRM mode, of the spiked urine samples at a resolution setting of 0.7 Da FWHM resulted in a Clenbuterol peak eluting from the column upon a broad chemical noise background signal containing interferent peaks from the urine.

The same urine samples analysed at a peak resolution setting of 0.1 Da FWHM resulted in elimination of the interfering isobaric mass peaks and the broad background chemical noise previously seen in the analysis at a peak width setting of 0.7 Da FWHM. The selected reaction monitoring analysis performed at a higher resolution setting of 0.1 Da FWHM resulted in increased selectivity of the assay and hence an increase in the precision could be achieved.

The increase in selectivity at a peak width setting of 0.1 Da FWHM is due to the fact that Clenbuterol is a chlorinated compound and thus the negative mass deficiency can be used to eliminate interferents from the urine matrix in SRM mode. This increased selectivity can be achieved without detrimental loss of transmission. Typically only a factor of two to three fold decrease in peak area is observed between analyses performed at 0.7 and 0.1 Da FWHM, however, greater selectivity could then be achieved.

The calibration curves for Clenbuterol concentrations of between 0.1 to 100 pg/L at resolution settings of 0.1 and 0.7 Da FWHM both demonstrate excellent linearity. The calibration line at 0.7 Da FWHM showed a high intercept due to chemical background in the urine blank. This was significantly reduced by the use of high resolution.

The use of higher resolution to increase selectivity and precision could enable the limit of quantitation of an assay to be lowered and achieves a higher degree of confidence in identification of analytes in biological matrices.
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature
2. Improved Sensitivity for Staining RNA with SYBR Gold Stain
3. Improved Vectors for PathDetect Trans-Reporting Systems
4. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
5. The Worlds Best DNase Improved TURBO DNA-free
6. Mx4000 Multiplex Quantitative PCR System*,PPP
7. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
8. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
9. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
10. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
11. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
Post Your Comments:
(Date:12/24/2014)... Texas (PRWEB) December 23, 2014 ... methyl mercaptan such as its definition, classification, ... presents product specification, manufacturing process, and product ... technology and applications. The analysis also covers ... marketing channels, industry development trend and proposals. ...
(Date:12/24/2014)... AUSTIN, Texas , Dec. 23, 2014 /PRNewswire/ ... solutions company focused on gynecologic disease, announced today ... W. Schuler , Birchview Fund LLC and several ... million of unregistered shares of Vermillion,s common stock ... common stock in a private placement.  ...
(Date:12/22/2014)... The Center for Professional Innovation ... training across the life sciences industry, has partnered with ... to provide the organization's members with discounted classroom courses, ... the more than 350 sessions across 80 course titles. ... when registering for a public course at ...
(Date:12/22/2014)... MISSION VIEJO, Calif. , Dec. 22, 2014 /PRNewswire/ ... announce that CoPatient, an online medical bill review and ... newest offering in the FREE WellCard Savings discount health ... a medical bill that was more than they expected ... help members reduce health care costs, WellCard Savings is ...
Breaking Biology Technology:Worldwide Methyl Mercaptan Market 2015-2020 Forecasts on Development & Trends Now Available at ReportsnReports.com 2Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 2Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 3Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 4Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 5CfPIE Announces Partnership with the Society for Clinical Data Management 2CfPIE Announces Partnership with the Society for Clinical Data Management 3WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2
... Truman Medical Centers (TMC),became one of the first hospitals ... AS 128 slice CT scanner this week. The 128-slice,CT ... with minimal radiation exposure to the patient., "To ... country to have this,truly, first of its kind, state-of-the-art ...
... - Enerkem Inc., a leading second,generation biofuels ... new Vice-Presidents to its executive team: Mr. ... Marie-Helene Labrie,Vice-President, Government Affairs and Communications., ... executives to our management,team," said Mr. Vincent ...
... Aug. 28 deCODE genetics,(Nasdaq: DCGN ) today ... deCODE,CSO Jeffrey Gulcher,s presentation at Thomas Weisel Partners Healthcare,Conference ... PM ET / 5:30 PM GMT / 6:30,PM British ... Seasons Hotel,Boston, MA. The webcast can be accessed through ...
Cached Biology Technology:Truman Medical Centers Becomes One of the First Hospitals in the U.S. To Install the World's First Adaptive 128-slice CT Scanner 2Enerkem Expands Executive Management Team 2deCODE genetics to Webcast Presentation at Thomas Weisel Partners Healthcare Conference 2008 2
(Date:11/21/2014)... , Nov. 19, 2014  Earlier this year ... New York College, and one of the most prolific ... signals that are transmitted from Smartphones to third party ... Smartphones and has one of the earliest known patents ... its usage in the military, child care, elder care ...
(Date:11/21/2014)... , Nov. 20, 2014   Atmel® Corporation ... microcontroller (MCU) and touch technology solutions, today launched the ... with the widest V cc range from ... accuracy and faster I 2 C bus communication speeds, ... EEPROM memory making them ideal for consumer, industrial, computer, ...
(Date:11/18/2014)... The Parenteral Drug Association (PDA) today confirmed that ... and at least seven more will participate in the upcoming ... Hotel in Washington D.C. , Dec. 2-4. ... support from the regulatory agencies in the United ... our effort to help advance the use of metrics in ...
Breaking Biology News(10 mins):Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 2Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 3Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 4FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2
... As 17 countries and 30 organizations launch ... major environmental groups World Wildlife Fund, The ... governments, businesses, scientists, non-governmental organizations and individuals around ... coral reefs. The International Year of the Reef ...
... Stowers Institutes Proteomics Center has published a novel method ... of affinity purifications analyzed by mass spectrometry (APMS) to ... to associate with one another. The ... not only to the interactions within well-defined protein assemblies, ...
... as many scientists had given up the search, researchers ... cells with the capacity to generate new insulin-producing beta ... for humans, the newfound progenitor cells will represent an ... diabetes, the researchers report in the Jan. 25 issue ...
Cached Biology News:Environmental groups call for increased protection of coral reefs 2Stowers Proteomics Center devises method for assigning probabilities to human protein interactions 2Elusive pancreatic stem cells found in adult mice 2