relation coefficient of r2=0.9990 with an equation of Y = 8496.82+266143*X was obtained for the curve. Figure 6: Clenbuterol curve at 0.7 Da FWHM The peak area, back-calculated values and precision of all calibration standards are shown in Figure 7. Figure 7: Calculated standards at 0.7 Da FWHM Experiment 2: Quantitative Analysis Performed at 0.1 Da FWHM The data below shows the quantitative analysis of Clenbuterol in Human urine at peak width settings of 0.1 Da FWHM on Q1 and 0.7 Da FWHM on Q3. Chromatograms are shown for blank urine (Figure 8) and urine containing Clenbuterol at 0.1 pg/L (Figure 9). Figure 8: Urine blank, 0.1 Da FWHM

Figure 9: Clenbuterol, 0.1 pg/L in urine, 0.1 Da FWHM Experiment 2: Quantitative Analysis Performed at 0.1 Da FWHM A calibration curve of Clenbuterol analysed at 0.1 Da FWHM was constructed using linear fit of peak area plotted against concentration, weighted 1/X (Figure 10). A correlation coefficient of r2=0.9994 with an equation of Y = 2661.76+85951.1*X was obtained for the curve. Figure 10: Clenbuterol curve at 0.1 Da FWHM The peak area, back-calculated values and precision of all calibration standards are shown in Figure 11. Figure 11: Calculated standards at 0.1 Da FWHM Discuss
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