Navigation Links
Improve RNA Isolation for Array Analysis and More

d WBCs make up only about 0.2% of blood cells and 2) whole blood also contains large amounts of proteins, heme, and genomic DNA, as well as intracellular and extracellular RNases. Thus, WBCs typically need to be isolated away from the rest of the blood constituents prior to RNA isolation.

The RiboPure-WBC Kit protocol consists of three steps: 1) fractionation of whole blood by centrifugation, recovery of the WBCs and RNA stabilization in Ambion's RNAlater (provided), 2) lysis of WBCs in guanidinium and initial RNA purification by phenol/chloroform extraction, and 3) final RNA purification on a glass fiber filter.

The kit provides sufficient reagents for the purification and stabilization of WBCs from 40 whole blood samples of up to 10 ml each (2.5-10 ml of whole blood can be used per isolation). The RNA purified with the RiboPure-WBC Kit can be used in the most demanding downstream applications, such as real-time RT-PCR or array analysis. Average yields of RNA range between 4-20 g from 5 ml of whole blood; the variation is due to differences in WBC numbers between samples.


back to top


Ordering Information
Cat# Product Name Size

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature
2. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
3. Improved Sensitivity for Staining RNA with SYBR Gold Stain
4. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
5. Improved Vectors for PathDetect Trans-Reporting Systems
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
8. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
9. Protect RNA and Improve Target Cell Identification
10. The Worlds Best DNase Improved TURBO DNA-free
11. Improve Recovery and Reproducibility with Pre-Treatment of Analytical TSK-GEL G3000SWXL Size Exclusion Columns
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Improve RNA Isolation for Array Analysis and More

(Date:7/29/2014)... July 29, 2014  United Therapeutics Corporation (NASDAQ: ... financial results for the second quarter ended ... shows that our medicines are reaching increasing ... hypertension (PAH)," said Martine Rothblatt, Ph.D., United ... commercial launch this quarter of our extended-release ...
(Date:7/29/2014)... Boston, MA (PRWEB) July 29, 2014 ... the Library of Congress was recently awarded to Juliana ... in Stoneham, MA, for their project, “From an Egg, ... from among 3,000 final entries for the National History ... more than 300 historians and educators in Washington, D.C. ...
(Date:7/29/2014)... CAMBRIDGE, England , July 29, 2014 ... das Liquid Biopsy System (LBS) zur Erfassung ... an den Herzkranzgefäßen entwickelt und patentiert. Das ... Stellen innerhalb der Koronararterie des Patienten und ... Das LBS hat das Potenzial, die Entdeckung ...
(Date:7/28/2014)... Texas (PRWEB) July 28, 2014 ... and China Lubricant Industry” is a professional ... Global Lubricant market. The report introduces Lubricant ... and industry chain structure and industry overview. ... including China’s domestic market as well as ...
Breaking Biology Technology:United Therapeutics Corporation Reports Second Quarter 2014 Financial Results 2United Therapeutics Corporation Reports Second Quarter 2014 Financial Results 3United Therapeutics Corporation Reports Second Quarter 2014 Financial Results 4United Therapeutics Corporation Reports Second Quarter 2014 Financial Results 5United Therapeutics Corporation Reports Second Quarter 2014 Financial Results 6United Therapeutics Corporation Reports Second Quarter 2014 Financial Results 7United Therapeutics Corporation Reports Second Quarter 2014 Financial Results 8United Therapeutics Corporation Reports Second Quarter 2014 Financial Results 9United Therapeutics Corporation Reports Second Quarter 2014 Financial Results 10United Therapeutics Corporation Reports Second Quarter 2014 Financial Results 11United Therapeutics Corporation Reports Second Quarter 2014 Financial Results 12United Therapeutics Corporation Reports Second Quarter 2014 Financial Results 13In Vitro Fertilization (IVF) was the Subject of This Year’s Winning Entry for the Prestigious Library of Congress “Discovery or Exploration in History Award” 2In Vitro Fertilization (IVF) was the Subject of This Year’s Winning Entry for the Prestigious Library of Congress “Discovery or Exploration in History Award” 3PlaqueTec sichert sich CE-Kennzeichnung für Liquid Biopsy System - bahnbrechende Innovation zur Erfassung neuer biologischer Informationen aus erkranktem Herzkranzgefäß 2PlaqueTec sichert sich CE-Kennzeichnung für Liquid Biopsy System - bahnbrechende Innovation zur Erfassung neuer biologischer Informationen aus erkranktem Herzkranzgefäß 3PlaqueTec sichert sich CE-Kennzeichnung für Liquid Biopsy System - bahnbrechende Innovation zur Erfassung neuer biologischer Informationen aus erkranktem Herzkranzgefäß 4Lubricant Market & Adhesion Promoter Industry (Globa, China) Analysis Now Available at DeepResearchReports.com 2Lubricant Market & Adhesion Promoter Industry (Globa, China) Analysis Now Available at DeepResearchReports.com 3Lubricant Market & Adhesion Promoter Industry (Globa, China) Analysis Now Available at DeepResearchReports.com 4
... for Presentation at ASCO, SOUTH SAN FRANCISCO, Calif., May ... it will hold an investor and,analyst briefing in conjunction with ... (ASCO) from 6:00 p.m. to 8:00 p.m. CT,on Monday, June ... PhD, President and Chief Executive Officer -- Michael M. ...
... Mass., May 21 Interleukin Genetics,Inc. (Amex: ... into law of the,Genetic Information Nondiscrimination Act (GINA). ... April 25, 2008, and signed into law by,President ... intended to ensure that individuals are protected,from genetic ...
... Germany, Mai 21, 2008 / b3c newswire ... both leading companies in biopharmaceutical development and ... The cooperation will enable Rentschler Biotechnologie’s clients ... mammalian biopharmaceutical production facilities in Biberach, Germany ...
Cached Biology Technology:Exelixis Announces June 2 Webcast of Its ASCO Investor and Analyst Briefing 2Exelixis Announces June 2 Webcast of Its ASCO Investor and Analyst Briefing 3Exelixis Announces June 2 Webcast of Its ASCO Investor and Analyst Briefing 4Interleukin Genetics Welcomes the Signing of the Genetic Information Nondiscrimination Act (GINA) 2Interleukin Genetics Welcomes the Signing of the Genetic Information Nondiscrimination Act (GINA) 3Rentschler Biotechnologie Announces Cooperation with Boehringer Ingelheim 2Rentschler Biotechnologie Announces Cooperation with Boehringer Ingelheim 3
(Date:7/27/2014)... has revealed that nicotine and cotinine, a metabolite of ... certain carcinogen in smoke. , The carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone or ... and ultimately ends up in the tobacco smoke. Once ... where it is activated by a variety of enzymes ... revealed that nicotine can partially interfere with the activation ...
(Date:7/27/2014)... can evade treatment by acquiring mutations in the genes ... were once thought to be the only way for ... shown that microorganisms can use a temporary silencing of ... the benefits of drug resistance without the commitment. , ... called Mucor circinelloides, it is likely to be employed ...
(Date:7/27/2014)... patients, scientists have identified more than two dozen ... six that had not been previously reported. The ... partially funded by the National Institutes of Health ... laboratories. , "Unraveling the genetic underpinnings of Parkinson,s ... in this complex disease, and hopefully, may one ...
Breaking Biology News(10 mins):Nicotine found to inhibit DNA-strand break caused by a certain carcinogen in smoke 2Scientists discover new, noncommittal mechanism of drug resistance 2Scientists discover new, noncommittal mechanism of drug resistance 3NIH scientists find 6 new genetic risk factors for Parkinson's 2
... ice-free summers with intermittent winter sea ice in the Arctic ... conditions - gives a glimpse of how the Arctic is ... past environmental change in the Arctic should help predict its ... 65 million years ago leading up to the extinction of ...
... PHILADELPHIA Researchers from the Centers for Disease Control ... ovarian cancer is influenced by age of menarche and ... suggests that hormonal activity over the course of a ... cancer diagnosis. Results of this study are published in ...
... Environmental Health Perspectives (EHP) co-authored by ... Project on Emerging Nanotechnologies (PEN), focuses on the ... provides an overview of current practices; research findings; ... and possible future directions for nanoremediation. The authors ...
Cached Biology News:Arctic climate under greenhouse conditions in the Late Cretaceous 2Arctic climate under greenhouse conditions in the Late Cretaceous 32 reproductive factors are important predictors of death from ovarian cancer 22 reproductive factors are important predictors of death from ovarian cancer 3Contaminated site remediation: Are nanomaterials the answer? 2
... unit ideal for use in laboratories with low ... set at 12 rpm , Temperature control ... capacity of four 35 x 150mm bottles, eight ... , Consistent, repeatable results achieved with microprocessor ...
... particle based purification systems provide high speed ... sample. They offer increased throughput and reproducible ... KingFisher, KingFisher mL and KingFisher 96, covering ... Each system consists of an instrument, specially ...
... DNA120OP is specifically engineered for efficient drying ... incorporates a glass cover and a post-trap ... unattended, odor-free processing of samples in ammonium ... Synthetic Oligos , ...
... is a no-frills, basic SpeedVac concentrator designed ... priced, this unit still features a standard ... display of time and heat. It is ... accepts a wide variety of different rotors ...
Biology Products: