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Improve RNA Isolation for Array Analysis and More

all RNA samples were treated with Ambion's DNA-free reagents to remove contaminating DNA.


Isolate High Quality Total RNA from Bacteria
As with yeast, bacterial gene expression patterns can also be affected during enzymatic or chemical pretreatments used to disrupt cell walls prior to RNA isolation. The RiboPure-Bacteria RNA Isolation Kit uses no enzymatic pretreatments, allowing for faster processing, which preserves RNA expression profiles. The kit utilizes the same type of protocol combining an efficient glass bead/one step phenol mediated disruption with the Vortex Adapter followed by a glass filter based RNA purification step. The kit has been used at Ambion to isolate high quality RNA from Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Campylobacter fetus, and Rhodobacter sphaeroides and is compatible with most bacterial RNA species (Figure 3). The RiboPure-Bacteria RNA Isolation Kit contains sufficient reagents for 50 RNA isolations, each from 107 up to 109 bacteria.

Figure 3. High Quality Bacterial RNA Obtained with RiboPure-Bacteria Kit. Total RNA (2 g) was separated on a 1% agarose gel in formaldehyde/MOPS buffer. Samples were heated at 80C for 10 min in denaturing agarose gel loading buffer with 100 g/l EtBr before electrophoresis at 5 V/cm.


RiboPure-WBC: Scalable Protocol for Isolating High Quality RNA from Blood
Isolating intact RNA from blood is challenging because: 1) only white blood cells (WBCs) contain appreciable amounts of RNA an
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