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Improve RNA Isolation for Array Analysis and More

choice for preparing RNA for use in microarray analysis or other procedures that require RNA of exceptional purity. Each of the RiboPure Kits described below are based on this dual method technology and also include Ambion's DNA-free genomic DNA removal system, making them ideal for preparation of RNA for RT-PCR based applications.


Fast Results and High Yields with Small Samples
The RiboPure Kit employs a rapid, 15 minute RNA purification procedure that combines the best features of one step organic extraction protocols and solid phase extraction techniques. From 1 to 100 mg of tissue or 100 to 107 cells can be processed per purification. Briefly, the protocol consists of disrupting samples in Ambion's RNAwiz, which contains phenol and RNase inhibitors, then adding chloroform and mixing. The sample is then centrifuged to separate organic and aqueous phases. The aqueous phase, which contains the RNA, is recovered and mixed with ethanol. This preparation is then passed over an RNA-binding silica filter. The filter is washed to remove contaminants, and the RNA is eluted in a small volume. A subsequent DNA removal step adds ~30 minutes of additional time (mostly incubation time) to the procedure. Total RNA yield is typically 80-400 g per 100 mg tissue, depending on the type of tissue processed. The RiboPure Kit contains reagents for up to 50 RNA isolations.


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