Navigation Links
Improve RNA Isolation for Array Analysis and More

choice for preparing RNA for use in microarray analysis or other procedures that require RNA of exceptional purity. Each of the RiboPure Kits described below are based on this dual method technology and also include Ambion's DNA-free genomic DNA removal system, making them ideal for preparation of RNA for RT-PCR based applications.


Fast Results and High Yields with Small Samples
The RiboPure Kit employs a rapid, 15 minute RNA purification procedure that combines the best features of one step organic extraction protocols and solid phase extraction techniques. From 1 to 100 mg of tissue or 100 to 107 cells can be processed per purification. Briefly, the protocol consists of disrupting samples in Ambion's RNAwiz, which contains phenol and RNase inhibitors, then adding chloroform and mixing. The sample is then centrifuged to separate organic and aqueous phases. The aqueous phase, which contains the RNA, is recovered and mixed with ethanol. This preparation is then passed over an RNA-binding silica filter. The filter is washed to remove contaminants, and the RNA is eluted in a small volume. A subsequent DNA removal step adds ~30 minutes of additional time (mostly incubation time) to the procedure. Total RNA yield is typically 80-400 g per 100 mg tissue, depending on the type of tissue processed. The RiboPure Kit contains reagents for up to 50 RNA isolations.


New! Isolate High Quality Total RNA from Yeast
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature
2. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
3. Improved Sensitivity for Staining RNA with SYBR Gold Stain
4. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
5. Improved Vectors for PathDetect Trans-Reporting Systems
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
8. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
9. Protect RNA and Improve Target Cell Identification
10. The Worlds Best DNase Improved TURBO DNA-free
11. Improve Recovery and Reproducibility with Pre-Treatment of Analytical TSK-GEL G3000SWXL Size Exclusion Columns
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Improve RNA Isolation for Array Analysis and More

(Date:1/15/2014)... 2014 TaiGen Biotechnology Company, Limited ("TaiGen") today announced ... a leading Russian pharmaceutical company, to develop and commercialize ... Federation , Turkey and ... a novel antibiotic for the treatment of bacterial infections ...
(Date:1/14/2014)... Histogen, Inc., a regenerative medicine company developing ... simulated embryonic conditions, today announced that they have entered ... for physician-dispensed aesthetic products containing Histogen’s proprietary multipotent cell ... an amendment to the existing license between Histogen and ...
(Date:1/14/2014)... York, NY (PRWEB) January 14, 2014 ... of Alliqua, Inc. (OTCQB: ALQA). Alliqua is an emerging ... to serve the wound care market. , Free report ... was restructured with a seasoned management team and Board, ...
(Date:1/14/2014)... 2014 iLab Solutions, the leader in core ... Director of Product Strategy. In this role, Michelle will work ... to guide in the development of iLab products. Her main ... possible benefit to the scientific community by offering the most ...
Breaking Biology Technology:TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 2Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 3EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2
... retains ownership of Pain Management business, NATICK, ... BSX ) today announced that it has completed ... business and drug pump,development program to former principals ... Bionics in 2004. The sale,coincides with the closing ...
... International,Associates, Inc., administrator for the HealthShares(TM) Indexes, a ... Funds,today announced that effective at the opening of ... MAXY ) will replace Aspreva Pharmaceutical,Inc. (Nasdaq: ... Inc. (NYSE: ALO ) will also replace ...
... with modest growth in the,traditional life science ... in agricultural, biodefense and molecular diagnostics. To,help ... and,advisory firm BioInformatics, LLC today released its ... of scientists working in,these dynamic markets. The ...
Cached Biology Technology:Boston Scientific Completes Sale of Auditory Business and Drug Pump Development Program 2Boston Scientific Completes Sale of Auditory Business and Drug Pump Development Program 3QED International Associates Announces Changes to the HealthShares(TM) Autoimmune-Inflammation and Neuroscience Indexes 2Study Examines Media Preferences of Life Scientists in Applied Markets 2Study Examines Media Preferences of Life Scientists in Applied Markets 3
(Date:4/24/2014)... It,s broadly understood that the world,s oceans play ... exchange of carbon between Earth,s ecosystems and atmosphere. ... UC San Diego have taken a leap forward ... , When phytoplankton use carbon dioxide to make ... material is released into the sea as a ...
(Date:4/24/2014)... COLUMBIA, Mo. When bacteria attack plants, they ... cells to weaken and suppress natural defenses. However, ... the plant cells as a potential host. Now, ... and replicated the process that allows the bacteriaknown ... discovery could lead to natural anti-infective treatments that ...
(Date:4/24/2014)... April 24, 2014GEN Publishing recently introduced ... focusing on the application of OMICs technologies in ... sequencing, are beginning to transform medical care just ... the past decade-and-a-half. , "GEN,s editors and reporters ... genomics, metabolomics, transcriptomics, etc. etc. for years," said ...
Breaking Biology News(10 mins):Microscopic organism plays a big role in ocean carbon cycling, Scripps scientists discover 2Microscopic organism plays a big role in ocean carbon cycling, Scripps scientists discover 3Plants send out signals attracting harmful bacteria, MU study finds 2
... at Rice University bolster the case for costly and ... in small populations of endangered species. , Worldwide, zoos ... thousands of miles in order to breed them with ... need for such programs, which can stress rare animals, ...
... challenges , Chemists have discovered details about how the tadpole-shaped ... into the top-most surface of water, an insight expected ... environmental hazards. The findings of a team led by ... on the cover of the Sept. 8 issue of the ...
... at the University of Pennsylvania have demonstrated that morphine ... enhancing virus replication. The paper by Wang et al., ... appears in the November issue of The American Journal ... , Hepatitis C virus (HCV) is common among ...
Cached Biology News:Costly breeding programs for endangered species pay off 2Costly breeding programs for endangered species pay off 3Scientists discover new way to look at how molecules twist and turn on water 2Hepatitis C complicated by morphine withdrawal 2
Application: for RNase- decontamination , Registered Trademark of Ambion. ,Special grade: for molecular biology Density: 1.00 g/mL (20 C) Suitab tst: RNase decontamination in accordance...
With L-glutamine and potassium bicarbonate. Sterile, Insect cell culture tested...
... meet the needs of the ... medium supports fast cell growth ... while maintaining high cell viability ... using the Baculovirus Expression Vector ...
Mol wt: average mol wt12,360.96 Da by calculation...
Biology Products: