Navigation Links
Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal,,,Fibroblasts


Improve transfection of primary cells up to 20-fold with Primary ENHANCER reagent

David Staggs
Genetic Applications LLC

Peter Pingerelli
Stratagene

Stratagenes Primary ENHANCER transfection reagent promotes efficient transfection of human dermal fibroblasts. Twenty minutes before transfection, cells are incubated in media containing the Primary ENHANCER reagent. After incubation, lipid or calcium phosphate (CaPO4)-mediated transfection can be performed using standard protocols. In earlier results, Primary ENHANCER reagent increased transfection efficiencies of some primary cell strains as high as 15-fold.1 In this study, we compared its effects on lipid- and CaPO4-mediated transfection using Stratagenes Normal Human Dermal Fibroblasts.

Human dermal fibroblasts are used to investigate many disease processes, including cancer, skin ulcers, collagen diseases, and scleroderma. They are also valuable for studying wound healing, growth factors, artificial skin, and dermal toxicity.

To study gene expression, a common approach usually involves introducing exogenous DNA into cells using transfection techniques in order to investigate the effects of resulting proteins. Unfortunately, primary human fibroblasts are difficult to transfect. However, with Stratagenes Human Dermal Fibroblasts, the method with which to study molecular and biochemical mechanisms in this system is improved. Additionally, Stratagenes new Primary ENHANCER reagent improves the transfection efficiency of lipid- or CaPO4-mediated transfection of human dermal fibroblasts.

Easily Increased Transfection

We grew Stratagenes Human Dermal Fibroblasts, which are isolated from adult skin and cryopreserved in vials (500,00 cells/vial),2 for two passages in standard
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature
2. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
3. Improved Sensitivity for Staining RNA with SYBR Gold Stain
4. Improved Vectors for PathDetect Trans-Reporting Systems
5. Improve Amplification Specificity with Hot Start PCR Enzyme
6. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
7. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
8. Improve RNA Isolation for Array Analysis and More
9. Protect RNA and Improve Target Cell Identification
10. The Worlds Best DNase Improved TURBO DNA-free
11. Improve Recovery and Reproducibility with Pre-Treatment of Analytical TSK-GEL G3000SWXL Size Exclusion Columns
Post Your Comments:
(Date:9/22/2014)... TORONTO , Sept. 22, 2014 ... announced that it has entered into a collaboration ... UHN,s Centre for Molecular Design and Preformulations (CMDP) ... The goal of the project is to increase ... proprietary buccal insulin spray product, thereby reducing the ...
(Date:9/22/2014)... Sept. 22, 2014  WIRB-Copernicus Group (WCG), the ... services for clinical research, announced today that its ... been reaccredited by the Association for the Accreditation ... to improve safeguards for clinical research participants. ... Deieso , Ph.D., commented, "It is of utmost ...
(Date:9/22/2014)... Synageva BioPharma Corp. ("Synageva") ... developing therapeutic products for rare disorders announced today ... Chief Operating Officer.  Reporting directly to Sanj ... Robert will oversee the planned global commercial launch ... LAL Deficiency, and will have responsibility for the ...
(Date:9/21/2014)... how to produce ultra-thin "diamond nanothreads" that promise extraordinary ... today,s strongest nanotubes and polymers. A paper describing this ... Badding, a professor of chemistry at Penn State University, ... of the journal Nature Materials . , "From ... because the threads we formed have a structure that ...
Breaking Biology Technology:Generex Collaborates with University Health Network for Buccal Insulin Project 2Generex Collaborates with University Health Network for Buccal Insulin Project 3Generex Collaborates with University Health Network for Buccal Insulin Project 4Western IRB (WIRB) Awarded AAHRPP Reaccreditation 2Synageva BioPharma Appoints Robert Bazemore As Chief Operating Officer 2Synageva BioPharma Appoints Robert Bazemore As Chief Operating Officer 3Synageva BioPharma Appoints Robert Bazemore As Chief Operating Officer 4Smallest possible diamonds form ultra-thin nanothreads 2Smallest possible diamonds form ultra-thin nanothreads 3Smallest possible diamonds form ultra-thin nanothreads 4
... via Single ... Platform, MirixaPro(SM), RESTON, Va. and WASHINGTON, Oct. ... creator of,the Diabetes Ten City Challenge and a partner in the ... that will,deliver all of APhA Foundation,s HealthMapRx commercial programs via,Mirixa. MirixaPro(SM) ...
... Oct. 9 eResearchTechnology,Inc. (eRT), (Nasdaq: ERES ... technology, ePRO and other services to the pharmaceutical,biotechnology, ... will release its 2008 third quarter results for ... 30, 2008 after the market closes.,After the release, ...
... CEL-SCI Corporation,(NYSE: CVM ) announced today ... facility for its lead drug Multikine(R). This dedicated,facility, ... Multikine that,will be used for CEL-SCI,s pivotal Phase ... head and neck cancer patients, and,subsequently for sale ...
Cached Biology Technology:Mirixa Corporation and the APhA Foundation Form Partnership 2Mirixa Corporation and the APhA Foundation Form Partnership 3eResearchTechnology, Inc. to Announce 2008 Third Quarter Results on October 30, 2008 2CEL-SCI Takes Delivery of New Manufacturing Facility 2
(Date:9/22/2014)... 2011 the Society of Environmental Toxicology and Chemistry (SETAC) ... and prioritize the scientific research needed to understand the ... the environment. The effort was extended, and results ... Society,s international journal, Integrated Environmental Assessment and Management ... a podcast interviewing the lead author of the study, ...
(Date:9/22/2014)... Additive manufacturing (AM), or 3D printing, has rapidly ... metal components strong enough for structural applications. However, ... as well as quality standards to test the ... technology. Engineers at the University of Pittsburgh,s Swanson ... modeling and simulation (M&S) technology and new qualification ...
(Date:9/22/2014)... and larger areas of sea ice melt in the summer ... it is thinner and more reduced. As the Arctic summers ... warming, because reduced sea ice in the Arctic will remove ... our results are representative, then sea ice plays a greater ... in future global CO2 budgets", says Dorte Haubjerg Sgaard, PhD ...
Breaking Biology News(10 mins):Priorities for research on pharmaceutical and personal care products in the environment 2Pitt engineers receive grants to enhance additive manufacturing 2Pitt engineers receive grants to enhance additive manufacturing 3Arctic sea ice helps remove CO2 from the atmosphere 2
... Nov. 21, 2011 Already partnering with 43 of ... Top 100 Innovators, NineSigma today announced a record year ... is critical to their long-term growth. "The ... never hesitate in their quest to innovate," said Andy ...
... data from electronic tags, ear bone microchemistry and fisheries catch ... of the Atlantic influences the other side. Bluefin populations ... since 1950, according to the study published today in the ... estimates the number of Atlantic bluefin tuna remaining in the ...
... FLAGSTAFF, Ariz. More peer-reviewed scientific studies ... developments and operations are needed to adequately assess their ... scientific literature review conducted by the U.S. Geological Survey ... In their literature review, the authors of the paper, ...
Cached Biology News:Surging Demand for Innovation Services Leads to Record Expansion for NineSigma 2Surging Demand for Innovation Services Leads to Record Expansion for NineSigma 3New study provides comprehensive view of the status of Atlantic bluefin tuna 2New study provides comprehensive view of the status of Atlantic bluefin tuna 3Literature review on solar energy and wildlife impacts research 2
... MULTI-FUN Incubator is a compact benchtop ... a microprocessor controller with a large ... and agitation speed. Air circulation inside ... incubator. Temperature can be easily calibrated ...
Anti Autotaxin /ENPP2 , - (Rabbit)...
Mouse polyclonal antibody to TNPO2 - transportin 2 (importin 3, karyopherin beta 2b)...
...
Biology Products: