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Immunoassay of Theophylline by fluorescence polarisation

inidine, salicylate, and acetaminophen. In this experiment, however, the antibodys specificity for a particular drug, combined with polarization measurements, eliminates interference from other fluorophores as well as unbound tags.

This example demonstrates that fluorescence immunoassay has, like radioimmunoassay, the specificity and sensitivity to perform at biologically relevant concentrations. The advantage of FIA, however, is that no separation step is required.


1 Visor, G.C.; Schulman, S.G. J. Pharm. Sci. 1981, 70, 469.
2 Smith, D.S.; Hassan, M.; Nargessi, R.D. In Modern Fluorescence Spectroscopy; Wehry, E.L., Ed.; vol. 3, 93, Plenum Press: New York, 1981.


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