Navigation Links
Immunoassay of Theophylline by fluorescence polarisation

ons.
The label should have at least one reactive functional group, such as isothiocyanate, acid chloride, or diazonium salt, to ease its conjugation to the ligand.
The label should not alter the specificity of the ligand for its antibody by shielding the reactive sites for antibody recognition.
The shelf-life and stability of the complex should be adequate for practical laboratory use.

Spectroscopic
The tag should have a strong absorption at the excitation wavelength of the experiment.
The quantum yield of the labeled species should be large.
The absorption and emission bands should be in the visible range to avoid interference from native proteins.
The separation between excitation and emission peaks should be large.

A further requirement of any immunoassay method is the possibility of distinguishing between free and bound labeled ligand, or the two species must be separated somehow. Here, FIA has a distinct advantage over RIA: by monitoring fluorescence polarization, the separation step is circumvented.

In fluorescence polarization, the excitation light is passed through a polarizing prism, and the emitted light is analyzed with another polarizer that is alternately oriented parallel and perpendicular to the excitation polarizer. The amount of polarization retained by the molecule depends upon its Brownian rotation between absorption and emission. A bound ligand will retain much of its polarization because the large molecular volume of the antibody gives the complex a long rotational relaxation time. The free ligand, on the other hand, has a much smaller volume, so the rotational relaxation time is shorter than its fluorescence lifetime. The emitted radiation, therefore, has a polari
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
2. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
3. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
4. Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System
5. Coated Plate Immunoassay on the MicroBeta - eg. TSH - IRMA
6. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Immunoassay Theophylline fluorescence polarisation

(Date:1/14/2014)... 14, 2014 The largest international professional organization ... and therapeutic derivatives thereof has endorsed an educational ... about the challenges of adulterated herb and botanical ... The Society for Medicinal Plant and ...
(Date:1/14/2014)... In recent years, growing suspicion about the ... development and promotion has led to unprecedented levels of ... concerns about the insidious impact of commercialization of research, ... to the world’s biggest pharmas for illegal marketing activities, ...
(Date:1/14/2014)... 14, 2014   Kinaxis ®, provider of RapidResponse ®, ... and operations planning ( S&OP ) service, is proud to ... which will be held at the Hilton San Diego Bayfront, ... customer Elisabeth Kaszas , Director of Supply Chain at ...
(Date:1/14/2014)... 14, 2014 During the 1600’s through the ... “The Doctor’s Plague.” In this time period, doctors did not ... at times, to the death of vulnerable patients. In the ... that they may be unwittingly transmitting herpes viruses to their ...
Breaking Biology Technology:World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 3Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 2Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 3
... More Effective Than rituximab ... in a Pre-Clinical ... announced today that ofatumumab (HuMax-CD20(R)) appeared more,effective at inducing complement ... a pre-clinical study. The CD20,antibodies were incubated with tumor cells ...
... YORK, Sept. 6 Globally, approximately 418 million,people ... danger, and costs of hepatitis in the 21st ... Hepatitis Strategies, a,new report by Kalorama Information. ... not,taking the necessary steps to contain hepatitis. Yet ...
... Globally, approximately 418 billion,people are currently infected with ... hepatitis in the 21st century cannot be,understated or ... by Kalorama Information. Paradoxically, despite the obvious ... to contain hepatitis. Yet the incidence and,prevalence of ...
Cached Biology Technology:Genmab Announces Encouraging Preclinical Data for ofatumumab 2Touching 418 Million Lives, Hepatitis Still Presents Significant Threat to Global Health 2Touching 418 Billion Lives, Hepatitis Still Presents Significant Threat to Global Health 2
(Date:4/18/2014)... For decades, scientists have used techniques like X-ray crystallography ... insight into the atomic structure of molecules, but such ... they demand large quantities of a specific molecule and ... making it all but impossible to peer into ... say those problems may soon be a thing of ...
(Date:4/18/2014)... twist to the story of how barnyard chickens came to ... the journal Proceedings of the National Academy of Sciences ... lived 200-2300 years ago in Europe, researchers report that just ... far different from the chickens we know today. , The ... modern domestic chickens -- such as their yellowish skin -- ...
(Date:4/18/2014)... A team of researchers led by a University ... to receive a $7.5 million Department of Defense grant ... basic scientific foundations for the predictable design of light-weight, ... diversity of structures from plants and animals, including the ... what biological systems have constructed over millions of years ...
Breaking Biology News(10 mins):MRI, on a molecular scale 2MRI, on a molecular scale 3Ancient DNA offers clues to how barnyard chickens came to be 2Mantis shrimp, toucan and trilobite, oh my 2Mantis shrimp, toucan and trilobite, oh my 3
... Holick, a professor of medicine, physiology and biophysics at ... the understanding of vitamin D and its role in ... Prize for Health Research. The prize was presented ... by the Linus Pauling Institute at Oregon State University. ...
... this summer,s lineup of lectures called Distinctive Voices@The ... Hole, Massachusetts, feature diverse subjects for those curious ... the program will explore science and technology,s role ... and creativity. Award-winning scientists and engineers -- ...
... made new inroads into understanding the regulatory circuitry of ... of daily activities, according to two studies published May ... clocks and temperature, reported in Cell , offers ... the 24-hour circadian rhythm does not change with temperature ...
Cached Biology News:Vitamin D expert receives Linus Pauling Prize for Health Research 2Woods Hole lecture series explores range of scientific topics 2Two new studies on circadian rhythms 2
... trinor Prostaglandin F2α isopropyl ester (15(R)-17-phenyl ... latanoprost-related isomer containing both a double ... (β) hydroxyl group at C-15. Similar ... ester is a potential impurity in ...
... new product number, created to easily match ... yet, please order under the old Sigma-Aldrich ... assistance. Feature: tissue-culture treated yes ... cm 2 D H 60 ...
... bind specifically to Fc regions of many ... conjugates are commonly used as affinity adsorbents ... from serum, hybridoma ascites fluids, tissue culture ... are also commonly used to capture immune ...
... min) assay can detect H2O2 or peroxidase ... H2O2 oxidizes ADHP in a 1:1 stoichiometry ... at 530-571 nm, emission at 590-600 nm). ... a one-step, homogeneous, no wash assay system. ...
Biology Products: