Navigation Links
Imaging and Analysis of DIGE Product Information Sheet, Rev B


Difference gel electrophoresis (DIGE) is a relatively straightforward application of differential labeling of protein samples using fluorescent dyes. The technique, originally published by Unlu et al. (1997), uses cyanine (Cy) dyes to differentially label proteins separated by 2-D gel electrophoresis. Following labeling and electrophoresis, excellent results can be achieved by scanning the multiplex gels with the Molecular Imager FX multiimager system and analyzing them with PDQuest 2-D analysis software.

The DIGE method is designed to compare two complex protein samples derived from two different growth conditions. One sample is labeled with Cy3 and the other with Cy5. A third dye, Cy2, is used to label a 50:50 mixture of the two samples, which becomes a standard for registering the images obtained with the other two dyes. Equal amounts of the three labeled protein mixtures are combined and run on the same 2-D polyacrylamide gel, eliminating any gel-to-gel variation.


Imaging
Image Acquisition With the Molecular Imager FX System
The Molecular Imager FX multiimager systems are well suited for image acquisition from gels containing Cy-dyed proteins. The laser and filter combinations provided ensure minimal cross-talk between the different fluors.

Lasers
The lasers required for the Cy dyes are the 488 nm external laser for Cy2, the 532 nm internal laser for Cy3, and the 635 nm external laser for Cy5.

Emission Filters
The required emission filters are 530BP30 for Cy2, 605DF50 for Cy3, and 695DF55 for Cy5.

Loading the Gels Into the Molecula
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
3. Imaging Chemiluminescent Western Blots
4. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
5. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
6. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
7. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
10. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
11. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Imaging and Analysis DIGE Product Information Sheet Rev

(Date:7/11/2014)... -- Research and Markets  has announced the addition ... Industry Analysis, Size, Share, Growth, Trends and Forecast to ... http://photos.prnewswire.com/prnh/20130307/600769 Transfection is a ... RNA) into cells. This technology helps the cells to ... metabolism by affecting the nuclear genes. The transfection technology ...
(Date:7/11/2014)... AZ (PRWEB) July 11, 2014 ... device company specializing in the soft tissue reconstruction ... All-Suture Anchor System for surgeries involving the shoulder ... fixation through a novel, inserter-controlled deployment method. The ... to partial deployment, anchor pull-out, or anchor displacement ...
(Date:7/10/2014)... Mass. (PRWEB) July 10, 2014 Terascala, ... today announced that Alan Swahn, a former vice president ... executive team as vice president of marketing. Terascala’s software ... Dell and NetApp create the highest performance and most ... been appointed to guide Terascala’s channel expansion and broaden ...
(Date:7/10/2014)... CA (PRWEB) July 10, 2014 Senior ... institutions and professional associations, addressed the challenges of “Reducing ... Medical Device Industries” through strategic sourcing. Describing the partnership ... with the Supply Chain Management Institute ( SCMI) ... event on June 24 at the Kroc Institute of ...
Breaking Biology Technology:International Transfection Technology Market - Industry Analysis, Size, Share, Growth, Trends and Forecast to 2019 2Cayenne Medical Launches SureLock™ All-Suture Anchor System for Shoulder and Extremity Surgery 2Cayenne Medical Launches SureLock™ All-Suture Anchor System for Shoulder and Extremity Surgery 3Terascala Expands Executive Team 2Terascala Expands Executive Team 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 2Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 4
... (the,"Company") (OTC Bulletin Board: TBIO) today announced financial results ... results are,presented in the tables that follow. Second ... of $0.2 million or $0.00 per share,for the second ... of $0.4 million or,$0.01 per share for the second ...
... Mass., Aug. 14 Avitar, Inc. (OTC,Bulletin Board: AVTI) ... ended June 30, 2007. For the quarter ended ... operations compared to $1,202,000 from continuing,operations for the quarter ... amounted to $597,000 versus $696,000. The net income,was $301,000, ...
... -- China Medical,Technologies, Inc. (the "Company") (Nasdaq: ... develops, manufactures and markets advanced in-,vitro diagnostic products ... announced that it will participate in the Morgan,Stanley,s ... Kong Date: August 21, 2007 China ...
Cached Biology Technology:Transgenomic, Inc. Reports Second Quarter 2007 Results 2Transgenomic, Inc. Reports Second Quarter 2007 Results 3Transgenomic, Inc. Reports Second Quarter 2007 Results 4Transgenomic, Inc. Reports Second Quarter 2007 Results 5Transgenomic, Inc. Reports Second Quarter 2007 Results 6Transgenomic, Inc. Reports Second Quarter 2007 Results 7Transgenomic, Inc. Reports Second Quarter 2007 Results 8Avitar Reports Third Quarter Financial Results for Fiscal 2007 2Avitar Reports Third Quarter Financial Results for Fiscal 2007 3Avitar Reports Third Quarter Financial Results for Fiscal 2007 4
(Date:7/14/2014)... have shown how the main psychoactive ingredient in cannabis ... Research published today reveals the existence of previously unknown ... in shrinking tumours. , It is hoped that ... anti-cancer properties. , The research was co-led ... used samples of human breast cancer cells to induce ...
(Date:7/13/2014)... of more effective treatments for cachexia, a profound wasting ... all cancer patients, raising their risk of death, according ... have been tried to reverse the condition, which may ... treatments, but none have had great success. , Scientists ... Nature , led by Bruce Spiegelman, PhD, demonstrated ...
(Date:7/11/2014)... awarded Michael F. Holick, PhD, MD, of Boston University ... Avioli Award. Holick, a professor of medicine, physiology and ... understanding of vitamin D and its role in disease ... the American Society for Bone and Mineral Research (ASBMR) ... It is named for ASBMR,s first president and founding ...
Breaking Biology News(10 mins):UEA research reveals how cannabis compound could slow tumour growth 2Antibody halts cancer-related wasting condition 2Antibody halts cancer-related wasting condition 3BUSM's Michael F. Holick receives American Society for Bone and Mineral Research award 2
... a drink problem, there is a greater risk that you ... from the Sahlgrenska Academy, at the University of Gothenburg, Sweden. ... 50% more likely to have a drink problem in the ... new light on this link. Carried out by researcher Anna ...
... LAWRENCE University of Kansas scientists have found new evidence ... (LCPUFA) are good for developing brains and hearts. In ... of four formulas from birth to 12 months; three with ... formula with no LCPUFA, and tested at four, six and ...
... our understanding of genetics and biological processes in all animals, ... of animals, scientists are still finding the fruit fly to ... the University of Rochester. The latest revelation has to ... fruit flies. It was widely accepted that all X chromosomes ...
Cached Biology News:New thinking on regulation of sex chromosomes in fruit flies 2
... These handy dispensers save space and ... and other garb. They are the ... parts used in labs, processing and ... charges and the particles they attract. ...
... 2 NaO 7 S 2 Formula Weight: ... Identity: By IR epsilon max (615 nm, ... equal to 60 x 10 3 Loss ... lambda max (H 2 O solution): ...
...
... research-grade spectrofluorometer with a minimum 1 nm ... 200 or greater for the Raman band ... to keep in mind in order to ... titration, or anisotropic measurements. ,The FP-6500 is ...
Biology Products: