Navigation Links
Imaging Chemiluminescent Western Blots

l ULTRA substrate had an integrated density measurement equivalent to a 216-ng band using ECL substrate, based on the equation of a standard curve generated when all four of the visible ECL bands were used as standards. This equates to an 11.5-fold increase in sensitivity observed when using SuperSignal ULTRA substrate compared to ECL reagent. These results suggest that researchers using the Eagle Eye II still video system to directly image filters from chemiluminescent Western blots may be able to increase sensitivity or decrease exposure time by using SuperSignal ULTRA substrate.

Conclusions

The Eagle Eye II still video system features a light-tight cabinet and a CCD camera that is sensitive to wavelengths of light emitted by common chemiluminescent substrates. These features make this system well suited for directly acquiring images from chemiluminescent Western blots. Western blot analyses performed using two different chemiluminescent substrates indicated that some substrates may perform better than others when used with the Eagle Eye II still video system. Consequently, researchers using the Eagle Eye II system to acquire images from chemiluminescent Westerns blots may be able to improve results by carefully choosing the chemiluminescent substrate.

Acknowledgments

The authors wish to thank Kerstien Padgett for helpful discussions concerning Western blot analysis.

REFERENCES
  1. Cayouette, M., Steege, M., and Moores, J. (1997) Strategies 10: 21-23.

  2. Gackstetter, T., et al. (1994) Strategies 7: 57-59.

  3. Mattson, D.L. (1996) Previews 1: 2-3.


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
3. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
4. Imaging and Analysis of DIGE Product Information Sheet, Rev B
5. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
6. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
7. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
10. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
11. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Imaging Chemiluminescent Western Blots

(Date:7/10/2014)... (PRWEB) July 10, 2014 Unraveling life’s ... together. The Archer Family purchased a DNA ... DNA, but ended up with a lifetime of memories, ... within the family members’ DNA genuinely brought the family ... to search deeper into genetic history and prior to ...
(Date:7/10/2014)... 10, 2014 Senior supply chain management ... associations, addressed the challenges of “Reducing Cost, Lead Time, ... through strategic sourcing. Describing the partnership of the Bio ... Chain Management Institute ( SCMI) of the University ... 24 at the Kroc Institute of Peace and Justice ...
(Date:7/10/2014)... 2014 /PRNewswire-iReach/ -- Tute Genomics, the leader ... an agreement with Lineagen, Inc., to provide ... Dx PLUS.  Lineagen, focused on the ... currently offers FirstStep Dx PLUS, a ... by healthcare providers as a first-line genetic ...
(Date:7/10/2014)... University of Adelaide research may help in the fight ... can detect tiny quantities of explosives with the use ... the journal Sensors and Actuators B: Chemical , ... can detect explosives in concentrations as low as 6.3 ... of only a few minutes. , "Traditionally explosives detection ...
Breaking Biology Technology:Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 2Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 3Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 4Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 5Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 2Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 4Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 3Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 4Detecting trace amounts of explosives with light 2
... Doping may be a no-no for athletes, but researchers ... was key in getting unprecedented power conversion efficiency from ... Graphene solar cells are one of industry,s great ... future. But previous attempts to use graphene, a single-atom-thick ...
... 25, 2012  Oncobiologics, Inc. announced today that it ... biopharmaceutical industry experts. Stephen J. McAndrew, Ph.D. is ... Benn, Ph.D. has joined the company as Vice ... announced its new Scientific Advisory Board, which will ...
... 24, 2012  For years, doctors and microbiologists have sent ... body for examination. These tests take days to get ... Donald Spector, a well-known, serial entrepreneur-inventor and Chairman of ... few institutionally accredited schools of Acupuncture and Massage Therapy ...
Cached Biology Technology:University of Florida physicists set new record for graphene solar cell efficiency 2Oncobiologics Fills Key Leadership Positions and Names Scientific Advisory Board 2Oncobiologics Fills Key Leadership Positions and Names Scientific Advisory Board 3College Chairman And Prolific Inventor Develops Life-Saving, Real-Time Diagnostic Technologies 2College Chairman And Prolific Inventor Develops Life-Saving, Real-Time Diagnostic Technologies 3
(Date:7/10/2014)... , July 3, 2014 Research ... addition of the "Global Gesture Recognition & ... - Forecasts to 2020" report to their ... Global Gesture Recognition & Touch-Less Sensing Market ... around for a while, but the companies were ...
(Date:7/10/2014)... 2014 According to a new ... Control Systems Market Global Forecast, Market Share, Size, Growth ... Access Control systems market was valued at USD 15,406.1 ... a CAGR of 12.6% from 2014 to 2019, to ... 2019. Browse the full Electronic Access Control ...
(Date:7/10/2014)... 2014  Acuity Market Intelligence today released forecasts from "The ... that the global market for National Electronic ID (eID) programs ... During this time, the number of National eID cards in ... Asia , with its vast population, will dominate ... issued, while Europe trails a distant ...
Breaking Biology News(10 mins):Global Gesture Recognition & Touch-Less Sensing (2D, 3D, Ultrasonic, IR, Capacitive) Market - Forecasts to 2020 2Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 2Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 3Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 4Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 5National Electronic ID Programs Generate $54 Billion Between 2013 and 2018 with 3.5 Billion National eID Card Holders Worldwide 2
... at Oregon State University has used a new method ... in Oregon with high numbers of methamphetamine-related problems per ... illegal drug. The study, presented today at a ... then identified five counties with the most meth-linked incidents ...
... PHILADELPHIA (September 15, 2008) More than 30 years ... of cancer genetics by showing that a person must ... particular class of cancer-inhibiting genes, called tumor-suppressor genes, in ... hypothesis, guided scientists around the globe in their quest ...
... (MGH) and Harvard Medical School investigator Gary Ruvkun, PhD, is ... Basic Medical Research. Presented by the Albert and Mary ... version of the Nobel Prize, and many Lasker recipients have ... be presented in New York on Friday, Sept. 26. ...
Cached Biology News:New method identifies meth hot spots 2New method identifies meth hot spots 3'1-hit' event provides new opportunity for colon cancer prevention, say Fox Chase researchers 2MGH researcher Gary Ruvkun named a co-recipient of the Lasker Award 2MGH researcher Gary Ruvkun named a co-recipient of the Lasker Award 3
Request Info...
... include: ,ICC and IHC Protocol Development ... ,Antibody Screenings in Animal or Human ... of Cells and Tissues ,Peroxidase, Alkaline ... Fluorescent, or Electron Microscopy ,Protein Localization ...
Request Info...
Request Info...
Biology Products: