Navigation Links
Imaging Chemiluminescent Western Blots

Use the Eagle Eye II still video system to directly acquire images from chemiluminescent blots

Michelle Cayouette Jane Moores
Stratagene

The Eagle Eye II still video system is well suited for direct imaging of chemiluminescent Western blots. The light-tight cabinet is ideal for the longer exposure times necessary to capture chemiluminescent signals. Also, the systems charge-coupled device (CCD) camera efficiently captures those wavelengths of light emitted by common chemiluminescent substrates. A comparison of chemiluminescent substrates from two manufacturers showed greater than a 10-fold difference in signal intensity. This difference indicates that by carefully choosing the appropriate chemiluminescent substrate for their system, researchers using the Eagle Eye II still video system to image chemiluminescent Western blots may be able to enhance their results.

The Eagle Eye II still video system is a powerful digital documentation and analysis tool for scientific researchers. The system combines quick and easy image acquisition with software processing functions, such as molecular weight determinations, densitometry calculations, and colony or plaque counting.1 Because of its light-tight cabinet and optimized camera sensitivity, the system also efficiently captures images directly from chemiluminescent experiments.2 Although the Eagle Eye II still video system can capture light emitted from the wide variety of substrates typically used in chemiluminescent Southern, Northern and Western blotting techniques, we have found that the substrate choice can have an impact on the quality of the resulting images.

Chemiluminescent Western Blots: Substrate Comparison

Chemiluminescent substrates were compared using the Eagle Eye II still video system to capture an image following Western blot analysis. Dilutions o
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
3. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
4. Imaging and Analysis of DIGE Product Information Sheet, Rev B
5. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
6. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
7. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
10. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
11. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Imaging Chemiluminescent Western Blots

(Date:9/18/2014)... Sept. 18, 2014   Sigma-Aldrich Corporation ... has entered into a new gene editing partnership ... Vector Core. Under the partnership, Sigma-Aldrich will provide ... design consultation, and dedicated gene editing bioinformaticians. ... editing-based research at the U-M Medical School. One ...
(Date:9/18/2014)... 2014 SeqLL Inc., the owner ... announced today the closing of a $1M Series-A ... Genomic Diagnostic Technologies, will increase the power of ... Founded in March 2013 by Daniel Jones, a ... Helicos BioSciences, SeqLL has continued to offer Helicos’ ...
(Date:9/18/2014)... lab can be pretty expensive. Now a team led ... an open-source library of designs that will let scientists ... equipment: the syringe pump. , Syringe pumps are used ... delivery or mixing chemicals in a reaction. They can ... Joshua Pearce and his team of Michigan Tech students ...
(Date:9/18/2014)... a new microscopy technology that allows them to ... resolution. , Dubbed "Complementation Activated Light Microscopy" (CALM), ... an order of magnitude finer than conventional optical ... biomolecules at the nanometer scale. , In a ... Communications , the researchers behind CALM used it ...
Breaking Biology Technology:Sigma-Aldrich, University of Michigan Medical School Establish CRISPR Core Lab Partnership 2Sigma-Aldrich, University of Michigan Medical School Establish CRISPR Core Lab Partnership 3Sigma-Aldrich, University of Michigan Medical School Establish CRISPR Core Lab Partnership 4SeqLL Closes Series-A Funding Round for Expansion of Single Molecule Sequencing 2Doing science just got cheaper -- and faster 2Scientists pioneer microscopy technique that yields fresh data on muscular dystrophy 2
... to Screen Donated Blood for Hepatitis B, as well ... and Semi-Automated Instrument Systems -, SAN DIEGO, Aug. ... that the US Food and Drug Administration,(FDA) has approved ... to screen donated blood for the hepatitis B,virus (HBV). ...
... Stock Exchange Symbol: MS, - Milestone triggers ... - EDMONTON, Aug. 13 /PRNewswire-FirstCall/ - BioMS ... treatment of multiple sclerosis (MS), today,announced that the ... trial has conducted the scheduled interim analysis of ...
... Mentice, Inc. and MATC have,forged a relationship that ... multiple,and mutually beneficial projects to further advance the ... throughout North America., Mentice,s Procedicus VIST Simulator ... physician to gain exposure to routine,and sophisticated procedures. ...
Cached Biology Technology:FDA Grants Full Approval of PROCLEIX(R) ULTRIO(R) Blood Screening Assay 2FDA Grants Full Approval of PROCLEIX(R) ULTRIO(R) Blood Screening Assay 3FDA Grants Full Approval of PROCLEIX(R) ULTRIO(R) Blood Screening Assay 4BioMS Medical announces positive interim analysis on phase III trial of dirucotide (MBP8298) for multiple sclerosis 2BioMS Medical announces positive interim analysis on phase III trial of dirucotide (MBP8298) for multiple sclerosis 3BioMS Medical announces positive interim analysis on phase III trial of dirucotide (MBP8298) for multiple sclerosis 4Mentice, Inc. and the Milwaukee Area Technical College (MATC) Announce Their Partnership in the Area of Procedural Medical Simulation 2
(Date:9/17/2014)... Calif. (Sept. 17, 2014) A rare genetic disorder known ... to a recent joint investigation by researchers at San ... Diego. In addition to suggesting better treatment options for ... clues into the genetic underpinnings of autism., Jacobsen syndrome ... National Institutes of Health. It occurs in a person ...
(Date:9/17/2014)... and their environmental impact recently scored a major win. ... on the bags, and Governor Jerry Brown is expected ... not yielding without a fight, according to an article ... newsmagazine of the American Chemical Society. , Alexander Tullo, ... campaign logged its first small victory in 1990 when ...
(Date:9/17/2014)... in Biomedical Informatics at IMIM (Hospital del Mar Medical ... (UPC) have recently published a study in eLife ... important role in the evolution of new proteins, some ... be discovered., Ribosomes produce proteins from the instructions found ... human genome is RNA containing information for the synthesis ...
Breaking Biology News(10 mins):A link between Jacobsen syndrome and autism 2Parts of genome without a known function may play a key role in the birth of new proteins 2
... African sanctuaries carry drug-resistant, human-associated strains of the bacteria ... chimpanzees could spread to endangered wild ape populations if ... study shows. The study by veterinarians, microbiologists and ... sequencing technology of bacterial genomes used in hospitals to ...
... forget-me-nots have been added to Flora of New Zealand . ... Island during an expedition led by Dr. Carlos A. Lehnebach. These ... the open access journal PhytoKeys . The expedition ... at the Museum of New Zealand Te Papa Tongarew a ...
... faces far greater change and risk of large-scale extinctions than ... the world,s leading marine scientists has warned. The researchers ... UK have compared events which drove massive extinctions of sea ... taking place in the seas and oceans globally today. ...
Cached Biology News:Sanctuary chimps show high rates of drug-resistant staph 2No ordinary forget-me-nots 2Sea life 'facing major shock' 2Sea life 'facing major shock' 3
... an inhibitor of caspase-3/7 ... of inhibitor required to ... be determined empirically for ... inhibitor is sufficient to ...
...
SNARE associated protein snapin, clone MGC:2717...
Human OSCAR MAb (Clone 259501)...
Biology Products: