Navigation Links
Imaging Chemiluminescent Western Blots

Use the Eagle Eye II still video system to directly acquire images from chemiluminescent blots

Michelle Cayouette Jane Moores
Stratagene

The Eagle Eye II still video system is well suited for direct imaging of chemiluminescent Western blots. The light-tight cabinet is ideal for the longer exposure times necessary to capture chemiluminescent signals. Also, the systems charge-coupled device (CCD) camera efficiently captures those wavelengths of light emitted by common chemiluminescent substrates. A comparison of chemiluminescent substrates from two manufacturers showed greater than a 10-fold difference in signal intensity. This difference indicates that by carefully choosing the appropriate chemiluminescent substrate for their system, researchers using the Eagle Eye II still video system to image chemiluminescent Western blots may be able to enhance their results.

The Eagle Eye II still video system is a powerful digital documentation and analysis tool for scientific researchers. The system combines quick and easy image acquisition with software processing functions, such as molecular weight determinations, densitometry calculations, and colony or plaque counting.1 Because of its light-tight cabinet and optimized camera sensitivity, the system also efficiently captures images directly from chemiluminescent experiments.2 Although the Eagle Eye II still video system can capture light emitted from the wide variety of substrates typically used in chemiluminescent Southern, Northern and Western blotting techniques, we have found that the substrate choice can have an impact on the quality of the resulting images.

Chemiluminescent Western Blots: Substrate Comparison

Chemiluminescent substrates were compared using the Eagle Eye II still video system to capture an image following Western blot analysis. Dilutions o
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
3. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
4. Imaging and Analysis of DIGE Product Information Sheet, Rev B
5. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
6. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
7. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
10. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
11. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Imaging Chemiluminescent Western Blots

(Date:9/2/2015)... September 2, 2015 BioLight ... or the "Company"), a firm that invests in, ... cancer diagnostics, announced today that presentations highlighting its ... be delivered at the Ophthalmology Futures European Forum ... of Cataract & Refractive Surgeons ("ESCRS"), both taking ...
(Date:9/2/2015)... Biovista Inc. freut sich, ... 2015 bekanntgeben zu können. Hierbei handelt es sich ... Biovista unterstützt mit seiner Möglichkeit der Analyse ... Krankheiten als denen, für die ein Medikament ursprünglich ... Team bei der Wylder Nation Foundation bei der ...
(Date:9/2/2015)... ... 2015 , ... Whitehouse Laboratories is excited to announce that ... upcoming educational seminar and training session on Extractable & Leachable testing. Featuring a ... Updates & Case Studies on the Latest Compliance Implications of USP and ICH ...
(Date:9/2/2015)... ... September 02, 2015 , ... The ... creating professional commercial video content, to analysis of athletic performance. Producing high-resolution ... go by too quickly to process with the naked eye. , Slow ...
Breaking Biology Technology:Presentations to Highlight BioLight's IOPtiMate[TM] System at Upcoming European Ophthalmology Conferences 2Presentations to Highlight BioLight's IOPtiMate[TM] System at Upcoming European Ophthalmology Conferences 3Biovista sponsert die Forschung von Wylder Nation Foundation zum Thema Mangel an saurer Sphingomyelinase 2Whitehouse Laboratories to Participate in Pharm Ed Resources Extractables, Leachables, & Elemental Impurities Educational Seminar 2Whitehouse Laboratories to Participate in Pharm Ed Resources Extractables, Leachables, & Elemental Impurities Educational Seminar 3Fastec Introduces the Quad-HD TS5-Q High-Speed Camera 2Fastec Introduces the Quad-HD TS5-Q High-Speed Camera 3
... part of a new strategic plan featuring enhancements to ... has announced the launch of nonstop Midwest Connect ... serves Research Triangle Park , beginning June 1. ... hostile takeover bid by AirTran Holdings, noted that Raleigh/Durham ...
... NimbleGen Systems announced on Friday afternoon that it has ... and number of shares are yet to be determined. J.P. ... Partners, Leerink-Swann & Company and Robert W. Baird & Co. ... $12.5 million venture capital investment at the close of 2006 ...
... comes to health information technology, Greg Smith doesn't have to ... is thinking. , ,Smith, senior vice president and CIO ... the business side of the Wheaton shop. Armed with a ... resource business, and now sits on Hewlett Packard's ...
Cached Biology Technology:CIO Leadership Series: Greg Smith, Wheaton Franciscan Healthcare 2CIO Leadership Series: Greg Smith, Wheaton Franciscan Healthcare 3CIO Leadership Series: Greg Smith, Wheaton Franciscan Healthcare 4
(Date:8/28/2015)... 2015 According to a new ... Assessment Type (Pen & Paper Based, Hosted, Biometrics), Service, ... Academic Research), Vertical and Region - Global Forecast to ... to grow from USD 2.4 Billion in 2015 to ... Growth Rate (CAGR) of 25.6% from 2015 to 2020. ...
(Date:8/20/2015)... 20, 2015 The wearable technology market ... active and healthy. However, wearable technology has the ... to help improve diagnostic capabilities and therapeutic outcomes. ... Wearable Technologies Conference 2015 , where ... wearables in healthcare.    "Over the ...
(Date:8/18/2015)... , Aug. 17, 2015 Research ... addition of the "Global Biometric Authentication & ... - Estimation & Forecast, 2015-2020" report to ... authentication and identification systems market is expected to ... 2015 to 2020 and generate over $25 billion ...
Breaking Biology News(10 mins):Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 2Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 3Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 4Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 2Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 3Global Biometric Authentication & Identification Market 2015: Focus On Modality, Motility & Application - Estimation & Forecasts to 2020 for the $25 Billion Industry 2
... presumed that the last glacial period denuded the Scandinavian ... weather began and melted away the ice cover some ... research headed by Professor Eske Willerslev from the Centre ... the University of Uppsala, Sweden, and Inger Greve Alsos ...
... potential genetic regulators in planarian stem cells, Whitehead Institute ... two main functions of stem cells. Three of the ... similar to those known to regulate mammalian embryonic stem ... attractive model for studying stem cell biology in vivo. ...
... Cut marks found on Ice Age bones indicate that humans ... known. Dr. Brian Redmond, curator of archaeology at The Cleveland ... in the Feb. 22, 2012 online issue of the journal ... animal bones found in 1998 in the collections of the ...
Cached Biology News:Sturdy Scandinavian conifers survived Ice Age 2Sturdy Scandinavian conifers survived Ice Age 3Planarian genes that control stem cell biology identified 2Planarian genes that control stem cell biology identified 3Research reveals first evidence of hunting by prehistoric Ohioans 2
GOAT ANTI HUMAN PARAINFLUENZA VIRUS 2+3...
... Cary 50 Microplate Reader accessory ... per analysis by extending the ... award-winning Cary 50 instruments. Use ... 384 samples in microlitre volumes, ...
IMAGEQUANT 300 WKSTN, 1 EA. Category: ImageQuant CCD Imagers....
PAb to Hu-IFN-Alpha/Beta R2 [(IFNAR2) CD118], Rabbit Serum, neutralizing Binds to but does not neutralize human interferon alpha/beta receptor...
Biology Products: